Clinico-pathological assessment of virulent Newcastle Disease Virus in ducks

Abstract

Newcastle disease (ND) is an infectious, highly contagious and lethal disease of avian species. It is considered that ducks are natural reservoir or carrier for Newcastle disease virus (NDV) and are resistant against different strains of NDV. Current study was designed to evaluate the pathogenesis of Newcastle disease in domestic ducks through histopathology, immunohistochemistry (IHC) and serum biochemical changes. For this purpose, eighty ducks were reared for 42 days and divided in two groups A and B. Ducks in group A were challenged with (NDV) at rate of 0.1 ml of ELD50 (virus titer 107.32/100µl) on second week of age, whereas Group B was control negative. Splenomegaly, atrophy of thymus and necrotic lesion in kidney were observed on 9th day of post infection. Hepatic degeneration and mononuclear cell infiltration were noticed in proventriculus and intestine in challenged ducks. Viral antigen detected in lungs, intestine, proventriculus and lymphoid organs of infected ducks through IHC. Albumin and total protein values were significantly low in infected groups A as compared to control group B. ALT, AST, and ALP values were significantly high in infected group A. On 5th and 7th day of post infection oropharyngeal swabs were negative for NDV and cloacal swabs were positive for NDV through Reverse transcriptase polymerase chain reaction. It is concluded that ducks are susceptible to NDV and virulent strain of NDV caused disease in ducks.

Gambaran Patologi Anatomi dan Histopatologi Organ Pertahanan Itik Bali Pascainfeksi Buatan Avian orthoavulavirus 1 Isolat Tabanan-1/ARP/2017

“…Pada penelitian sebelumnya perubahan patologi organ pertahanan ayam yang diinfeksi virus ND virulen menunjukkan derajat infeksi yang lebih parah. Ayam yang terinfeksi virus ND virulen menyebabkan splenomegali, hemorrhagi, nekrosis, dan deplesi sel limfoid pada organ limpa; serta kerusakan yang parah pada folikel limfoid timus dan bursa Fabricius (Adi et al, 2019a;Zahid et al, 2021). Namun perubahan yang terjadi pada itik cenderung bersifat ringan sampai sedang (Zahid et al, 2021).…”

“…Ayam yang terinfeksi virus ND virulen menyebabkan splenomegali, hemorrhagi, nekrosis, dan deplesi sel limfoid pada organ limpa; serta kerusakan yang parah pada folikel limfoid timus dan bursa Fabricius (Adi et al, 2019a;Zahid et al, 2021). Namun perubahan yang terjadi pada itik cenderung bersifat ringan sampai sedang (Zahid et al, 2021). Lebih rendahnya derajat keparahan yang terjadi terutama pada organ pertahanan itik dibandingkan Buletin Veteriner Udayana Volume 14 No.…”