Modulating the production of xylanase by Bacillus pumilus BS131 through optimization using waste fiber sludge

Abstract

In recent days, cheapest alternative carbon source for fermentation purpose is desirable to minimize production cost. Xylanases have become attractive enzymes as their potential in bio-bleaching of pulp and paper industry. The objective of the present study was to identify the potential ability on the xylanase production by locally isolated Bacillus pumilus BS131 by using waste fiber sludge and wheat bran media under submerged fermentation. Culture growth conditions were optimized to obtain significant amount of xylanase. Maximum xylanase production was recorded after 72 hours of incubation at 30 °C and 7 pH with 4.0% substrate concentration. In the nutshell, the production of xylanase using inexpensive waste fiber sludge and wheat-bran as an alternative in place of expensive xylan substrate was more cost effective and environment friendly.

A linear and circular dual-conformation noncoding RNA involves in oxidative stress tolerance of Bacillus altitudinis

“…Bacillus strains are important industrial enzyme producers 16 , and many commercial enzymes including proteases, amylases, xylanases and lipases have been characterized and purified from different Bacillus species [17][18][19][20] . To explore the post-transcriptional regulation in Bacillus strains, we previously identified a set of sRNAs in Bacillus altitudinis SCU11, a productive strain of extracellular alkaline protease, through an RNA-seq-based approach and subsequent validation by northern blot 21 .…”