Antinociceptive and anti-inflammatory activities of Hymenaea martiana Hayne (Fabaceae) in mice

Abstract

Hymenaea martiana is a species popularly known in Northeastern Brazil as “jatobá” and used in folk medicine to treat pain and inflammation. The aim of this work was to evaluate the antinociceptive and anti-inflammatory activity of H. martiana. In the present study, we carried out an investigation about the effects of the crude ethanolic extract (Hm-EtOH) and the ethyl acetate fraction (Hm-AcOEt) in models of nociception and inflammation in mice. Chemical (acetic acid-induced writhing and formalin) and thermal stimuli (hot plate) were used for the evaluation of antinociceptive activity, while for the anti-inflammatory profile paw edema induced by carrageenan was used, along with leukocyte migration to the peritoneal cavity. The presence of the flavonoid astilbin in the samples was characterized through HPLC-DAD-MS analysis. Hm-EtOH and Hm-AcOEt (100, 200 and 400 mg.kg-1, i.p.) significantly reduced the number of abdominal contortions and decreased the paw licking time in the formalin test. In the hot plate, the extract increased the latency time of animals. Hm-EtOH and Hm-AcOEt inhibited significantly the increase in the edema after the administration of carrageenan. Hm-EtOH and Hm-AcOEt inhibited leukocyte migration in the peritonitis test. HPLC-DAD-MS analysis of Hm-EtOH and Hm-AcOEt revealed the presence of the flavonoid astilbin in the samples. According to the results of this study, both Hm-EtOH and Hm-AcOEt have antinociceptive and anti-inflammatory activities, which could be related with the presence of flavonoid in the extracts. The results reinforce the popular use of this plant.

Dual quinoline-hybrid compounds with antimalarial activity against Plasmodium falciparum parasites

“…All statistical analyses were carried out using Graph-Pad Prism s v.5.0 software (GraphPad Prism Software Inc., San Diego, CA, USA, https://www.graphpad.com/scientific-software/ prism/). 60,61 3. Results and discussion…”

“…All statistical analyses were carried out using GraphPad Prism® v.5.0 software (GraphPad Prism Software Inc., San Diego, CA, USA, ). 60,61…”

“…Furthermore, the kit sensitivity is 8 pg mL À1 (IL-1b). 60,61 2.6.6 Measurement of ROS production. The production of ROS was measured using a dichlorofluorescein diacetate (CM-H2DCFDA; Invitrogen) probe.…”

Composição química do extrato etanólico das folhas de Hymenaea martiana Hayne (FABACEAE) e seu potencial no combate a larvas e pupas de Aedes Aegypti

“…Essa espécie, conhecido popularmente como "jatobá" é comumente encontrada na região nordeste do Brasil e possui diversas atividades comprovadas como: antinociceptiva e antiinflamatória (PACHECO et al, 2021), antioxidante (OLIVEIRA et al, 2021), antibacteriana (PEIXOTO et al, 2016) e hepatoprotetora (CLOSA et al, 1997).…”