Assessment of zinc solubilization potential of zinc-resistant Pseudomonas oleovorans strain ZSB13 isolated from contaminated soil

Rehman, H.; Ashraf, Ayesha; Muzammil, Saima; Siddique, Muhammad Hussnain; Ali, Tayyaba

doi:10.1590/1519-6984.240015

Cited by 2 publications

References 26 publications

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Antibiotic and heavy metal resistant endophytes inhabit Armeria maritima hyperaccumulator

Goryluk-Salmonowicz

Myczka²,

Popowska³

2023

Preprint

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Background and Aims The occurrence of heavy metal-resistant bacteria in plants and their role in phytoremediation intensification has been quite well recognized in the last few years. Nevertheless, there is a dearth of information on antibiotic resistance profile of those bacteria. In this study, for the first time endophytic bacteria has been isolated from green parts of Armeria maritima sp. halleri plant growing on mine-tailing soil in the southern Poland. The resistance profile of bacteria was researched. Methods Bacteria were isolated from internal tissues of Armeria maritima plant and characterized: MIC was determined by the plate dilution method using (CH3COO)2Pb and ZnSO4 supplemented medium; antibiotic susceptibility test was determined by disk diffusion method according to the EUCAST version 11.0; the whole genome sequencing was performed on MiSeq platform (Illumina). Physicochemical properties of soil were evaluated according to European Standards. Results Five Pseudomonas sp. strains exhibiting high tolerance to heavy metals were resistant to antibiotics, such as aminoglycosides, ß-lactam antibiotics, fosfomycines fluorochinolones, macrolides and glycopeptides. Draft genome sequences analysis estimated genome sizes in a range from 6,182,403bp to 7,401,235bp and a G + C content from 60.0–61.0%. 12 and 11 genes conferring resistance heavy metals and antibiotics were identified, respectively. Conclusion Armeria maritima subsp. halleri is inhabited by resistant to heavy metals and antibiotic endophytic bacteria identified as Pseudomonas species. Under the One Health concept the contamination of soil and plants with ARB and ARGs should be monitored and limited and a regulatory framework for safety use of bacterial bioinoculants should be established.

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Antibiotic and heavy metal resistant endophytes inhabit Armeria maritima hyperaccumulator

Goryluk-Salmonowicz

Myczka²,

Popowska³

2023

Preprint

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Finger Millet (Eleusine coracana) Plant–Endophyte Dynamics: Plant Growth, Nutrient Uptake, and Zinc Biofortification

et al. 2023

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Endophytic fungi and bacteria were isolated from finger millet and their effects on finger millet growth parameters and zinc and NPK contents in grains were studied. Out of 70 fungal and 112 bacterial endophytes, the two best fungal and bacterial isolates were selected on the basis of zinc solubilization and plant-growth-promoting attributes. The fungal isolates identified were Aspergillus terreus and Lecanicillium sp., and the bacterial isolates were Pseudomonas bijieensis and Priestia megaterium. The endophytic zinc, NPK mobilization, and plant-growth-promoting efficacy were determined in a pot experiment with zinc carbonate as the zinc source. Endophytic-primed plants showed enhanced shoot and root lengths compared to the unprimed control. Endophytes increased the zinc content in grains by between 12.12% and 18.80% compared to control plants. Endophytes also augmented the NPK concentrations in seeds compared to control plants and exhibited stability in a diverse range of pHs, temperatures, and NaCl concentrations, and exhibited growth on various carbohydrate and nitrogen sources. This is the first study reporting the interaction of Aspergillus terreus, Lecanicillium sp., Pseudomonas bijieensis, and Priestia megaterium with finger millet for grain Zn biofortification and NPK concentration enhancement. This study indicated that zinc-dissolving endophytes possess the potential for enhancing the zinc and NPK content in grains in addition to the plant-growth-promoting attributes.

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Assessment of zinc solubilization potential of zinc-resistant Pseudomonas oleovorans strain ZSB13 isolated from contaminated soil

Cited by 2 publications

References 26 publications

Antibiotic and heavy metal resistant endophytes inhabit Armeria maritima hyperaccumulator

Antibiotic and heavy metal resistant endophytes inhabit Armeria maritima hyperaccumulator

Finger Millet (Eleusine coracana) Plant–Endophyte Dynamics: Plant Growth, Nutrient Uptake, and Zinc Biofortification

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