Induction of secondary metabolites on nanoparticles stress in callus culture of Artemisia annua L.

Abstract

Nanoparticles are known bio elicitors in plant biotechnology. Different concentrations of ZnO, CuO and CoO nanoparticles were used for the enhanced accumulation of secondary metabolites and antioxidant activities in the callus derived from root, shoot and leaf of Artemisia annua L. Biomass of callus was somehow affected on high concentrations of Nps. Phenolic content was observed maximum (60µg) in shoot callus at 0.1mg/l of CuONps. Total antioxidant activity was observed maximum (33µg) in root callus at 0.1mg/l of ZnOnps. Total reducing power maximum (33µg) was observed in root callus at concentration of 0.05 mg/l of CoONps. Maximum radical scavenging activity was observed in shoot callus at 0.05mg/l of ZnONps. Rutin gallic acid and caffic acid were also determined in most of the samples by HPLC. The study concludes that different Nps have positive effect on the induction of secondary metabolites in A.annua plant.

Dietary Supplementation of Enzymatically Treated Artemisia annua L. Improves Lactation Performance, Alleviates Inflammatory Response of Sows Reared Under Heat Stress, and Promotes Gut Development in Preweaning Offspring

“…Disrupted physiological balance and reduced milk production and feed conversion efficiency of sows under HS have also been observed (43). To date, many previous studies have shown that diets supplemented with A. annua extract or A. annua leaf powder increased ADG and ADFI (14,23,44) and alleviated HS-induced body weight reduction and intestinal morphology impairment in broilers (19). Beyond this, emerging evidence also demonstrated that a diet directly supplemented with EA increased ADG and nutrient digestibility in weaned pigs (45,46).…”

“…Approximately 600 secondary metabolites have been identified and separated in A. annua, including sesquiterpenes, monoterpenes, flavonoids, and phenolic compounds. Among these compounds, artemisinin characterizes the biological action of A. annua (19). In addition, many previous studies have shown that A. annua powder or extract could enhance growth performance (20), anti-inflammation (21)(22)(23), redox status, and innate immunity in broilers and rats (24,25).…”

Foliar Application of Selenium under Nano Silicon on Artemisia annua: Effects on Yield, Antioxidant Status, Essential Oil, Artemisinin Content and Mineral Composition

“…Among wormwood species, Artemisia annua L. attracts special attention due to its effectiveness against malaria, cancer [1], inflammation [2] and oxidative stresses [3,4]. This plant is rich with terpenoids; phenolic compounds, such as coumarins and flavonoids [5,6]; and lipopolysaccharides [2], and is considered to be the only plant source of artemisininthe main sesquiterpene with a powerful antimalarial effect [7,8]. This sesquiterpene lactone is very effective against chloroquine-resistant strains of P. falciparum malaria and also against various types of cancers and tumors; some viruses, including SARS-CoV-2; tuberculosis; and many other parasites, including Schistosoma sp., Leishmania sp., Borrelia burgdorferi and Trypanosoma sp.…”

Phytochemical Analysis and Anti-Inflammatory Activity of Different Ethanolic Phyto-Extracts of Artemisia annua L.

“…Glandular trichomes can be found both in leaves and the stem of AA and be rich in biologically active secondary metabolites. More than 600 secondary metabolites have been identified in AA, including sesquiterpenes, monoterpenes, and phenolic compounds, such as coumarins and flavonoids [3]. In particular, more than thirty sesquiterpenes have been identified in AA, which are mainly located in the aerial part [3,4].…”

“…More than 600 secondary metabolites have been identified in AA, including sesquiterpenes, monoterpenes, and phenolic compounds, such as coumarins and flavonoids [3]. In particular, more than thirty sesquiterpenes have been identified in AA, which are mainly located in the aerial part [3,4]. Artemisinin (ART), isolated for the first time in 1972 by the Nobel Prize winner Youyou and then structurally defined in 1979 [5], is the main sesquiterpene that, together with its derivatives, characterizes the biological action of AA.…”