Antibacterial and antiproliferative activities of the fresh leaf essential oil of Psidium guajava L. (Myrtaceae)

Abstract

This study evaluated the antibacterial and antiproliferative activities of the essential oil of Psidium guajava leaves (PG-EO), traditionally used in folk medicine. The essential oil was obtained from fresh leaves by hydrodistillation, using a modified Clevenger apparatus. The major PG-EO chemical constituents were identified by GC-MS and GC-FID as being β-caryophyllene (16.1%), α-humulene (11.9%), aromadendrene oxide (14.7%), δ-selinene (13.6%), and selin-11-en-4α-ol (12.5%). The antibacterial activity of the essential oil of P. guajava leaves was determined in terms of its minimum inhibitory concentrations (MIC) using the broth microdilution method in 96-well microplates. PG-EO had moderate activity against Streptococcus mutans (MIC = 200 µg/mL), S. mitis (MIC = 200 µg/mL), S. sanguinis (MIC = 400 µg/mL), S. sobrinus (MIC = 100 µg/mL), and S. salivarius (MIC = 200 µg/mL). The antiproliferative activity was evaluated against different tumor cell lines: breast adenocarcinoma (MCF-7), human cervical adenocarcinoma (HeLa), and human gliobastoma (M059J). A normal human cell line (GM07492A, lung fibroblasts) was included. The antiproliferative activity was evaluated using the XTT assay and the results were expressed as IC50. The essential oil showed significantly lower IC50 values against MCF-7 and M059J lines than that obtained for the normal line, showing selectivity. Our results suggest that the essential oil of Psidium guajava L. has promising biological activities and can be considered a new source of bioactive compounds.

Chemical characterization and antimicrobial activity of Campomanesia aurea against three strains of Listeria monocytogenes

“…Oxygenated monoterpenes are shown to be the principal constituents of essential oils, possessing antimicrobial activity against various microorganisms, implying L. monocytogenes, which can be specifically related to the chemical constituents 4-terpineol and 1,8-cineole; a result, which was also encountered in this study (Castro et al, 2016;Silva et al, 2019).…”

Pharmacognostical, biochemical activities and zootechnical applications of Psidium guajava (Myrtaceae), plant with high medicinal value in tropical and subtropical parts of the World: A review

“…Majoritary compounds depend on the sampling stations Benin [22] Leave β-bisabolene, ar-curcumene βbisabolol Sesquiterpenes Hydrocarbon compound Hydrocarbon sesquiterpenes Brazil [25] Leave β-caryophyllene α-humulene aromadendrene oxide δ-selinene selin-11-en-4α-ol Egypt [9] D-Limonen α-Pinene…”

Portrayal of Punica granatum L. peel extract through High Performance Liquid Chromatography and antimicrobial activity evaluation

“…Different plant extracts have been reported for their antimicrobial activities including some of the important appended here i.e., Aframomum melegueta (Doherty et al, 2011), aqueous and hydroalcoholic extracts of Acanthospermum australe (Loefl.) Kuntze against diarrhea-inducing bacteria (Mallmann et al, 2018), antibacterial and antiproliferative activities of the fresh leaf essential oil of Psidium guajava L. (Silva et al, 2019) and antimicrobial activity of Campomanesia aurea against three strains of Listeria monocytogenes (Pacheco et al, 2020).…”