Phytochemical screening and in vitro antibacterial, antifungal, antioxidant and antitumor activities of the red propolis Alagoas

Abstract

The study aimed to evaluate the antimicrobial activity, antioxidant, toxicity and phytochemical screening of the Red Propolis Alagoas. Antimicrobial activity was evaluated by disk diffusion method. Determination of antioxidant activity was performed using the DPPH assay (1.1-diphenyl-2-picrylhydrazyl), FTC (ferric thiocyanate) and determination of phenolic compounds by Follin method. Toxicity was performed by the method of Artemia salina and cytotoxicity by MTT method. The phytochemical screening for the detection of allelochemicals was performed. The ethanol extract of propolis of Alagoas showed significant results for antimicrobial activity, and inhibitory activity for Staphylococcus aureus and Candida krusei. The antioxidant activity of the FTC method was 80% to 108.3% hydrogen peroxide kidnapping, the DPPH method showed an EC50 3.97 mg/mL, the content of total phenolic compounds was determined by calibration curve gallic acid, resulting from 0.0005 mg/100 g of gallic acid equivalent. The extract was non-toxic by A. salina method. The propolis extract showed high activity with a higher percentage than 75% inhibition of tumor cells OVCAR-8, SF-295 and HCT116. Chemical constituents were observed as flavonones, xanthones, flavonols, and Chalcones Auronas, Catechins and leucoanthocyanidins. It is concluded that the extract can be tested is considered a potential source of bioactive metabolites.

Antifungal and Antibacterial Effect of Propolis: A Comparative Hit for Food-Borne Pseudomonas, Enterobacteriaceae and Fungi

“…The antimicrobial activity of propolis has been extensively reviewed by different authors; it is well known that it is able to inhibit and/or control the growth of a wide range of microorganisms, either Gram positive (Listeria monocytogenes, Staphylococcus spp., Streptococcus spp., Bacillus spp.) or Gram negative bacteria (Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae, Pseudomonas aeruginosa), as well as yeasts and molds (Candida spp., Aspergillus spp., Penicillium digitatum, Saccharomyces cerevisiae, Cladosporium spp., Trychophyton spp., Alternaria alternata and Fusarium oxysporum) [2][3][4][5][6][7][8]. Propolis is composed of more than 300 different components, like polyphenol (flavonoids, phenolic acids and esters), phenolic aldehydes and ketones.…”

Brazilian Red Propolis: Extracts Production, Physicochemical Characterization, and Cytotoxicity Profile for Antitumor Activity

“…A red variety of Brazilian propolis has been described in the last years, with the ethanolic extracts being enriched in isoflavones, mainly formononetin, daidzein, and biochanin A [7]. Studies have demonstrated that such hydroalcoholic and ethanolic extracts have potential antitumoral activity both in vitro [8,9] and in vivo [10][11][12][13][14][15].…”

Phytochemical analysis and biological activities of ethanolic extract of Curcuma longa rhizome

“…Although many traditional medicinal plants are effective in decreasing blood sugar, most of these are not practically utilized in severe diabetes (Ranilla et al, 2010). Many plants have shown biological activities and utilized as standardized extracts (Dar et al, 2019;Silva et al, 2019;Pontes et al, 2019).…”