Morphobiological, morphometric and ultrastructural characterization of sylvatic Trypanosoma cruzi isolates from Rio de Janeiro state, Brazil

Silva, C. Santos da; Carbajal-de-la-Fuente, Ana Laura; Almeida, Carlos Eduardo; Gonçalves, Teresa Cristina Monte; Santos-Mallet, Jacenir Reis dos

doi:10.1590/1519-6984.181719

Cited by 4 publications

(2 citation statements)

References 31 publications

(35 reference statements)

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“…It has also been determined that the loss of glucose in culture is the trigger for flagellar elongation (Tyler & Engman, ). Lengthening of cells and flagella is hypothesized to be “preadaptive” for metacyclogenesis (Hernandez et al, ; Tyler & Engman, ), although the connection remains obscure and the natural isolates contain a mix of insect life stages that are extremely morphologically varied within and between isolates (da Silva, Carbajal‐de‐la‐Fuente, Almeida, Gonçalves, & dos Santos‐Mallet, ; Kollien and Schaub, ).…”

Section: Resultsmentioning

confidence: 99%

A link between mitochondrial gene expression and life stage morphologies in Trypanosoma cruzi

Ramirez-Barrios

Susa

Smoniewski

et al. 2020

Molecular Microbiology

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The protozoan Trypanosoma cruzi has a complicated dual-host life cycle, and starvation can trigger transition from the replicating insect stage to the mammalianinfectious nonreplicating insect stage (epimastigote to trypomastigote differentiation). Abundance of some mature RNAs derived from its mitochondrial genome increase during culture starvation of T. cruzi for unknown reasons. Here, we examine T. cruzi mitochondrial gene expression in the mammalian intracellular replicating life stage (amastigote), and uncover implications of starvation-induced changes in gene expression. Mitochondrial RNA levels in general were found to be lowest in actively replicating amastigotes. We discovered that mitochondrial respiration decreases during starvation in insect stage cells, despite the previously observed increases in mitochondrial mRNAs encoding electron transport chain (ETC) components. Surprisingly, T. cruzi epimastigotes in replete medium grow at normal rates when we genetically compromised their ability to perform insertion/deletion editing and thereby generate mature forms of some mitochondrial mRNAs. However, these cells, when starved, were impeded in the epimastigote to trypomastigote transition. Further, they experience a short-flagella phenotype that may also be linked to differentiation. We hypothesize a scenario where levels of mature RNA species or editing in the single T. cruzi mitochondrion are linked to differentiation by a yet-unknown signaling mechanism. K E Y W O R D S gene expression, life cycle stages, organelles, ribonuclease, RNA editing, trypanosomiasis S U PP O RTI N G I N FO R M ATI O N Additional supporting information may be found online in the Supporting Information section. How to cite this article: Ramirez-Barrios R, Susa EK, Smoniewski CM, Faacks SP, Liggett CK, Zimmer SL. A link between mitochondrial gene expression and life stage morphologies in Trypanosoma cruzi. Mol Microbiol.

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Section: Resultsmentioning

confidence: 99%

A link between mitochondrial gene expression and life stage morphologies in Trypanosoma cruzi

Ramirez-Barrios

Susa

Smoniewski

et al. 2020

Molecular Microbiology

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“…This disease has a marked medical and socioeconomic impact, since there are about 8 million people who are infected by T. cruzi and around 10,000 deaths annually worldwide, is responsible for high rates of morbidity, affecting around six to eight million people in Latin America and with 90-120 million people at risk. (Garcia et al, 2016;Silva et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Validation of a documentary on Chagas disease by a population living in an endemic area

et al. 2021

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Educational interventions may trigger actions that contribute to prevent parasitic diseases, such as Chagas disease (CD). This study aimed at investigating the impact of an instructional video named “Documentary on Chagas Disease” on knowledge about CD and its vectors displayed by a population that lives in an endemic area in Brazil, so as to validate it as an educational tool. The video was shown to 226 subjects, divided into two groups. Group 1 was composed of users of Basic Health Units (BHU) in Pelotas and Pinheiro Machado, cities located in Rio Grande do Sul (RS) state, Brazil, where CD is endemic. Group 2 consisted of students who attend three public schools located in the rural area in Pinheiro Machado, RS. Two questionnaires with questions about their knowledge about triatomines and CD were applied, before and after the documentary was shown. After the video was shown, there was significant increase in individuals’ knowledge (in both groups) about “kissing bugs”, their notification, the disease and its prevention. Besides, watchers considered that the quality of the material was satisfactory. Since the “Documentary on CD” can be easily accessed on the internet and was effective in teaching the population that lives in endemic areas, its use should be encouraged in places and meetings connected to health that aim at fighting against triatominae and at exposing an updated view of CD.

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A link between mitochondrial gene expression and life stage morphologies inTrypanosoma cruzi

Ramirez-Barrios

Susa

Faacks

et al. 2019

Preprint

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SummaryThe protozoan Trypanosoma cruzi has a complicated dual-host life cycle, and starvation can trigger transition from the replicating insect stage to the mammalian-infectious nonreplicating insect stage (epimastigote to trypomastigote differentiation). Abundance of some mature RNAs derived from its mitochondrial genome increase during culture starvation of T. cruzi for unknown reasons. Here we examine T. cruzi mitochondrial gene expression in the mammalian intracellular replicating life stage (amastigote), and uncover implications of starvation-induced changes in gene expression in insect-stage cells. Mitochondrial RNA levels in general were found to be lowest in actively replicating amastigotes. We discovered that mitochondrial respiration decreases during starvation, despite the previously-observed increases in mitochondrial mRNAs encoding electron transport chain components. Surprisingly, T. cruzi epimastigotes in replete medium grow at normal rates when we genetically compromised their ability to perform insertion/deletion editing and thereby generate mature forms of some mitochondrial mRNAs. However, these cells, when starved, were impeded in the epimastigote to trypomastigote transition. Further, they experience a short-flagella phenotype that may also be linked to differentiation. We hypothesize a scenario where levels of mature RNA species or editing in the single T. cruzi mitochondrion are linked to differentiation by a yet-unknown signaling mechanism.

show abstract

Morphobiological, morphometric and ultrastructural characterization of sylvatic Trypanosoma cruzi isolates from Rio de Janeiro state, Brazil

Cited by 4 publications

References 31 publications

A link between mitochondrial gene expression and life stage morphologies in Trypanosoma cruzi

A link between mitochondrial gene expression and life stage morphologies in Trypanosoma cruzi

Validation of a documentary on Chagas disease by a population living in an endemic area

A link between mitochondrial gene expression and life stage morphologies inTrypanosoma cruzi

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