Impact of phosphate solubilizing bacteria on wheat (Triticum aestivum ) in the presence of pesticides

Abstract

Three phosphate solubilizing bacteria were isolated and identified by 16S rRNA sequencing as Pseudomonas putida, Pseudomonas sp and Pseudomonas fulva . The strains were subjected to plant biochemical testing and all the PGPR attributes were checked in the presence of pesticides (chlorpyrifos and pyriproxyfen). The phosphate solubilizing index of strain Ros2 was highest in NBRIP medium i.e 2.23 mm. All the strains showed acidic pH (ranges from 2.5-5) on both medium i.e PVK and NBRIP. Strain Ros2 was highly positive for ammonia production as well as siderophore production while strain Rad2 was positive for HCN production. The results obtained by the strains Rad1, Rad2 and Ros2 for auxin production were 33.1, 30.67 and 15.38 µg ml-1, respectively. Strain Rad1 showed 16% increase in percentage germination in comparison to control in the presence of pesticide stress. Most promising results for chlorophyll content estimation were obtained in the presence of carotenoids upto 6 mgg-1 without stress by both strains Rad1 and Rad2. Study suggests that especially strain Ros2 can enhance plant growth parameters in the pesticide stress.

The Impact of Growth-Promoting Streptomycetes Isolated from Rhizosphere and Bulk Soil on Oilseed Rape (Brassica napus L.) Growth Parameters

“…Similar results have been reported in studies where bacterial strains tested on Pikovskaya agar showed a low solubility index [73][74][75]. The ability of the same bacteria to dissolve phosphates on solid agar and liquid medium differs; some studies have found that the bacteria show a considerably higher phosphate production in liquid medium [76,77]. The low phosphate solubility index may be related to several factors, such as organic acid production or nutrient composition [76,78], but according to the available source, it is not related to origin of the strains.…”

Production of Bacillibactin Siderophore from Soil Bacteria, Bacillus subtilis: A Bioinoculant Enhances Plant Growth in Arachis hypogaea L. Through Elevated Uptake of Nutrients

“…The siderophores producing bioinocultant treated to mung bean plants protect the plant by reducing the chlorotic lesions on the leaves and also increased the chlorophyll levels under iron-deficient stress [32]. Likewise, increase carotenoids level was observed in siderophores producing strain (Ros2) treated to wheat plants in the presence of pesticide stress [33]. [34] isolated a potential siderophores producing organism from rhizosphere, this bacterium treated to peanut plants showed drastic increase in the seed germination, shoot, root length and also recoded enhanced levels of chlorophyll.…”

Sorghum-Phosphate Solubilizers Interactions: Crop Nutrition, Biotic Stress Alleviation, and Yield Optimization

“…The PSM controls the phytopathogens attack by synthesizing pivotal molecules like siderophores ( Sarr et al, 2020 ), antibiotics ( Raaijmakers and Mazzola, 2012 ), lytic enzymes ( Herrera-Quiterio et al, 2020 ), cyanogenic compounds ( Munir et al, 2019 ), and through induction of systemic resistance (ISR; Audenaert et al, 2002 ). Due to this, the soil PSM improves the growth and grain yield of sorghum by suppressing the damaging impact of biotic stresses.…”