Antifungal activity of linalool in cases of Candida spp. isolated from individuals with oral candidiasis

Abstract

This study analyzed the antifungal activity of phytoconstituents from linalool on Candida spp. strains, in vitro, isolated from patients with clinical diagnoses of oral candidiasis associated with the use of a dental prosthesis. Biological samples were collected from 12 patients using complete dentures or removable partial dentures and who presented mucous with diffuse erythematous or stippled features, indicating a clinical diagnosis of candidiasis. To identify fungal colonies of the genus Candida, samples were plated onto CHROMagar Candida  . The antifungal activity of linalool, a monoterpene unsaturated constituent of basil oil, was performed using the broth microdilution technique. Then, the minimum inhibitory concentration (MIC), the two subsequent stronger concentrations and the positive controls were subcultured on Sabouraud Dextrose Agar plates to determine the minimum fungicidal concentration (MFC). The experiments were performed in triplicate and nystatin was used as a positive control in all tests. Diagnoses of oral candidiasis were verified in eight patients (66.6%) and the most prevalent fungal species was Candida albicans (37.5%), followed by Candida krusei (25.0%); and Candida tropicalis (4.2%). The best antifungal activity of linalool was observed on Candida tropicalis (MIC = 500 mg/mL), followed by Candida albicans (MIC = 1.000 mg/mL), and Candida krusei (MIC = 2.000 mg/mL).Under the study conditions and based on the results obtained, it can be concluded that the Candida strains tested were susceptible to linalool.Keywords: candidiasis, linalool, phytotherapy. Atividade antifúngica do linalol em casos de Candida spp. isolados de indivíduos com candidíase oral ResumoEste estudo analisou a atividade antifúngica do fitoconstituinte linalol em cepas de Candida ssp, in vitro, isolados de pacientes com o diagnóstico clínico de candidíase oral associado ao uso de prótese dentária. As amostras biológicas foram coletadas de 12 pacientes portadores de próteses totais ou próteses parciais removíveis e que apresentavam características de mucosa eritematosa difusa ou pontilhadas, indicando um diagnóstico clínico de candidíase. Para identificar colônias de fungos do gênero Candida, as amostras foram semeadas em CHROMagar Candida  . A atividade antifúngica do linalol, um componente insaturado monoterpene de óleo de manjericão, foi realizada através da técnica de microdiluição em caldo. Em seguida, a concentração inibitória mínima (MIC), as duas concentrações consecutivas mais fortes e os controles positivos foram subcultivados em placas de Agar Sabouraud Dextrose para determinar a concentração fungicida mínima (MFC). Os experimentos foram realizados em triplicata e a nistatina foi usada como controle positivo em todos os testes. O diagnóstico de candidíase oral foi comprovado em oito pacientes (66,6%) e as espécies de fungos mais prevalentes foram Candida albicans (37,5%), seguido por Candida krusei (25,0%); e Candida tropicalis (4,2%). A melhor atividade antifúngica do linalol foi observada em Candi...

Analysis of the distribution and antifungal susceptibility of Candida albicans and non-albicans Candida isolated from human blood culture

“…18 Several other studies reported that they were commonly detected among patients aged between 50 and 79 years. [19][20][21] In our study, the mean age was found to increase with year, but it was not considered significant (p=0.1294).…”

Anticandidal Properties of Lavandin Essential Oils

“…In vitro study found that oil of L. angustifolia inhibited growth of C. albicans, as did it is component linalool (D' Auria et al, 2005). The efficacy of clinical use of linalool has been confirmed in several Candida strains (C. albicans, C. krusei, and C. tropicalis) that contaminated the oral cavity in patients with oral candidiasis (Dias et al, 2018). Its' constituents linalool and linalyl acetate are detectable in the blood five minutes after topical application and largely disappear from the blood within 90 minutes.…”

Chemical Variation and Pharmacological Properties of Dyssodia decipiens Essential Oil

“…Published reports on the effectiveness of linalool on clinical isolates of C. albicans supported the weak inhibitory effects found in the present investigation. [13] For both cases (linalool and verbenone), high amounts were required to inhibit the in vitro growth of assayed C. albicans strains.…”