Alcanivorax dieselolei, an alkane-degrading bacterium associated with the mucus of the zoanthid Palythoa caribaeorum (Cnidaria, Anthozoa)

Campos, Felipe Ferreira; García, José Eduardo; Luna-Finkler, Christine Lamenha; Davolos, Camila Chiaradia; Lemos, Manuel Victor Franco; Pérez, Carlos Daniel

doi:10.1590/1519-6984.16113

Cited by 5 publications

(4 citation statements)

References 26 publications

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“…The Vibrionales OTU identified were assigned to the genus Pseudoalteromonas or to the family Pseudoalteromonadaceae , and were detected only in the Apapa sediment. Other known PAH/hydrocarbon degraders were Alcanivorox ( Luan et al, 2014 ; Campos et al, 2015 ) and Halomonas ( Gasperotti et al, 2015 ; Gutierrez et al, 2015 ). In the Eledu sediment, there was only one Oceanospirillales OTU ( Oleibacter ) and in the Ofin site there were no OTU identified as Oceanospirillales .…”

Section: Resultsmentioning

confidence: 99%

Microbial Communities in Sediments of Lagos Lagoon, Nigeria: Elucidation of Community Structure and Potential Impacts of Contamination by Municipal and Industrial Wastes

et al. 2016

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Estuarine sediments are significant repositories of anthropogenic contaminants, and thus knowledge of the impacts of pollution upon microbial communities in these environments is important to understand potential effects on estuaries as a whole. The Lagos lagoon (Nigeria) is one of Africa’s largest estuarine ecosystems, and is impacted by hydrocarbon pollutants and other industrial and municipal wastes. The goal of this study was to elucidate microbial community structure in Lagos lagoon sediments to identify groups that may be adversely affected by pollution, and those that may serve as degraders of environmental contaminants, especially polycyclic aromatic hydrocarbons (PAHs). Sediment samples were collected from sites that ranged in types and levels of anthropogenic impacts. The sediments were characterized for a range of physicochemical properties, and microbial community structure was determined by Illumina sequencing of the 16S rRNA genes. Microbial diversity (species richness and evenness) in the Apapa and Eledu sediments was reduced compared to that of the Ofin site, and communities of both of the former two were dominated by a single operational taxonomic unit (OTU) assigned to the family Helicobacteraceae (Epsilonproteobacteria). In the Ofin community, Epsilonproteobacteria were minor constituents, while the major groups were Cyanobacteria, Bacteroidetes, and Firmicutes, which were all minor in the Apapa and Eledu sediments. Sediment oxygen demand (SOD), a broad indicator of contamination, was identified by multivariate analyses as strongly correlated with variation in alpha diversity. Environmental variables that explained beta diversity patterns included SOD, as well as levels of naphthalene, acenaphthylene, cobalt, cadmium, total organic matter, or nitrate. Of 582 OTU identified, abundance of 167 was significantly correlated (false discovery rate q≤ 0.05) to environmental variables. The largest group of OTU correlated with PAH levels were PAH/hydrocarbon-degrading genera of the Oceanospirillales order (Gammaproteobacteria), which were most abundant in the hydrocarbon-contaminated Apapa sediment. Similar Oceanospirillales taxa are responsive to marine oil spills and thus may present a unifying theme in marine microbiology as bacteria adapted for degradation of high hydrocarbon loads, and may represent a potential means for intrinsic remediation in the case of the Lagos lagoon sediments.

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Section: Resultsmentioning

confidence: 99%

Microbial Communities in Sediments of Lagos Lagoon, Nigeria: Elucidation of Community Structure and Potential Impacts of Contamination by Municipal and Industrial Wastes

et al. 2016

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“…The Alcanivorax genus presently comprises 11 species, of which A. dieselolei (Liu and Shao, 2005) represents one of the most environmentally spread. A. dieselolei was firstly isolated in 2001 from Bohai Sea surface waters and deep-sea sediments of the Pacific Ocean (Liu and Shao, 2005), and it has been detected in a wide range of marine costal and pelagic environments (Wang et al, 2010; Kostka et al, 2011), tropical and temperate areas (Wang et al, 2010; Kostka et al, 2011; Hassanshahian et al, 2012), in association with other marine organisms (Campos et al, 2015), as well as in hydrocarbon-contaminated saline soils (Dastgheib et al, 2011). The distribution of this specie in the environment mirrors its physiological traits, which allow its representatives to adapt to a wide range of different environmental conditions.…”

Section: Introductionmentioning

confidence: 99%

Hydrocarbonoclastic Alcanivorax Isolates Exhibit Different Physiological and Expression Responses to n-dodecane

et al. 2016

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Autochthonous microorganisms inhabiting hydrocarbon polluted marine environments play a fundamental role in natural attenuation and constitute promising resources for bioremediation approaches. Alcanivorax spp. members are ubiquitous in contaminated surface waters and are the first to flourish on a wide range of alkanes after an oil-spill. Following oil contamination, a transient community of different Alcanivorax spp. develop, but whether they use a similar physiological, cellular and transcriptomic response to hydrocarbon substrates is unknown. In order to identify which cellular mechanisms are implicated in alkane degradation, we investigated the response of two isolates belonging to different Alcanivorax species, A. dieselolei KS 293 and A. borkumensis SK2 growing on n-dodecane (C12) or on pyruvate. Both strains were equally able to grow on C12 but they activated different strategies to exploit it as carbon and energy source. The membrane morphology and hydrophobicity of SK2 changed remarkably, from neat and hydrophilic on pyruvate to indented and hydrophobic on C12, while no changes were observed in KS 293. In addition, SK2 accumulated a massive amount of intracellular grains when growing on pyruvate, which might constitute a carbon reservoir. Furthermore, SK2 significantly decreased medium surface tension with respect to KS 293 when growing on C12, as a putative result of higher production of biosurfactants. The transcriptomic responses of the two isolates were also highly different. KS 293 changes were relatively balanced when growing on C12 with respect to pyruvate, giving almost the same amount of upregulated (28%), downregulated (37%) and equally regulated (36%) genes, while SK2 transcription was upregulated for most of the genes (81%) when growing on pyruvate when compared to C12. While both strains, having similar genomic background in genes related to hydrocarbon metabolism, retained the same capability to grow on C12, they nevertheless presented very different physiological, cellular and transcriptomic landscapes.

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“…In the present study, the bacterium was identified from coral isolated from Qeshm Island. 31 The data obtained in this study showed that high concentrations of crude oil can reduce the ability of bacteria to degrade. Furthermore, high concentrations of hydrocarbons limit the bioavailability of oxygen, nitrogen, and phosphorus, which reduces the growth of bacteria and, thus, reduces degradation.…”

Section: Wileyonlinelibrarycom/jctbmentioning

confidence: 67%

Biodegradation of crude oil using symbiont crude‐oil degrading bacteria isolated from corals collected at the Persian Gulf

Ansari

Rokhbakhsh‐Zamin

Hassanshahian

et al. 2021

J of Chemical Tech & Biotech

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BACKGROUND: About 25,000 oil tankers are transported annually through the Persian Gulf in Iran. Therefore, oil pollution in this region is very high. Research on the use of marine bacteria to degrade crude oil has yielded good results in recent years. The present study investigates bacteria that are symbiotic with marine corals and their ability to degrade crude oil. Corals were collected from five regions in the Persian Gulf and enriched with decomposing bacteria in ONR7a culture. After biochemical and molecular identification of bacterial isolates, the ability of the strains to degrade crude oil by spectrophotometric, gravimetric, and Gas Chromatography-Mass Spectroscopy (GC-MS) methods was investigated.RESULTS: Finally, 26 bacterial strains were identified from 5 coral samples. The efficient strains that had the highest crude-oil degradation belonged to genera Cobetia, Shewanella, Alcanivorax, and Cellulosimicrobium. Strains IAUK3568, IAUK3568, IAUK3502, IAUK3531, and IAUK3552 exhibited a maximum degradation of crude oil of 93.5%, 88.13%, 87.24%, 85.17%, and 77.3%, respectively. CONCLUSION:The results showed that all bacterial isolates degrade medium-chain alkanes better than long-and short-chain alkanes. The data obtained in this study confirm that the corals of the Persian Gulf have the necessary bacterial diversity to decompose crude oil and, consequently, reduce pollution in this region.

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Alcanivorax dieselolei, an alkane-degrading bacterium associated with the mucus of the zoanthid Palythoa caribaeorum (Cnidaria, Anthozoa)

Cited by 5 publications

References 26 publications

Microbial Communities in Sediments of Lagos Lagoon, Nigeria: Elucidation of Community Structure and Potential Impacts of Contamination by Municipal and Industrial Wastes

Microbial Communities in Sediments of Lagos Lagoon, Nigeria: Elucidation of Community Structure and Potential Impacts of Contamination by Municipal and Industrial Wastes

Hydrocarbonoclastic Alcanivorax Isolates Exhibit Different Physiological and Expression Responses to n-dodecane

Biodegradation of crude oil using symbiont crude‐oil degrading bacteria isolated from corals collected at the Persian Gulf

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