Proteomic profile of Piper tuberculatum (Piperaceae)

Abstract

Piper tuberculatum (Piperaceae) is a species that accumulates especially amides as secondary metabolites and several biological activities was previously reported. In this article, we report a proteomic study of P. tuberculatum. Bidimensional electrophoresis (2D SDS-PAGE) and mass spectrometry (ESI-Q-TOF) were used in this study. Over a hundred spots and various peptides were identified in this species and the putative functions of these peptides related to defense mechanism as biotic and abiotic stress were assigned. The information presented extend the range of molecular information of P. tuberculatum.Keywords: Piperaceae, amidas, Piper tuberculatum, proteomics, 2D-SDS-PAGE. Perfil proteômico e metabólico de Piper tuberculatum (Piperaceae) ResumoPiper tuberculatum (Piperaceae) é uma espécie que acumula especialmente amidas como metabólitos secundários e diversas atividades biológicas dessa espécie foram relatadas anteriormente. No presente artigo, relatamos um estudo proteômico dessa espécie. Eletroforese bidimensional (2D SDS-PAGE) e espectrometria de massas (ESI-Q-TOF) foram utilizadas nesse estudos. Mais de cem spots e vários peptídeos foram identificados nesta espécie e as funções putativas desses peptídeos relacionadas a mecanismo de defesa como estresse biótico e abiótico foram atribuídos. As informações apresentadas ampliam a gama de informações moleculares dessa espécie.Palavras-chave: Piperaceae, amides, Piper tuberculatum, proteômica, 2D SDS-PAGE.

An unprecedented chlorine-containing piperamide from Piper pseudoarboreum as potential leishmanicidal agent

“…Consequently, there has been a recent increase in the use of plant derived natural products as source of leishmanicidal agents [37]. Compounds isolated from P. pseudoarboreum are closely related to those reported from Piper tuberculatum, which exhibit interesting antileishmanial profiles [38]. Furthermore, it has been suggested that the structural characteristic of alkamides confer on them specific biological activities [17].…”