Biochemical identification techniques and antibiotic susceptibility profile of lipolytic ambiental bacteria from effluents

Abstract

Different methodologies have been developed throughout the years to identify environmental microorganisms to improve bioremediation techniques, determine susceptibility profiles of bacteria in contaminated environments, and reduce the impact of microorganisms in ecosystems. Two methods of bacterial biochemical identification are compared and the susceptibility profile of bacteria, isolated from residential and industrial wastewater, is determined. Twenty-four bacteria were retrieved from the bacteria bank of the Environmental Microbiology Laboratory at the Institute of Biology (IB) of the Universidade Federal de Pelotas, Pelotas, Brazil. Bacteria were identified by conventional biochemical tests and by the VITEK ®2 automated system. Further, the susceptibility profile to antibiotics was also determined by the automated system. Six species of bacteria (Raoutella planticola, K. pneumoniae ssp. pneumoniae , Serratia marcescens, Raoutella sp., E. cloacae and Klebsiella oxytoca) were identified by conventional biochemical tests, while three species of bacteria (K. pneumoniae ssp. pneumoniae, S. marcescens and K. oxytoca ) were identified by VITEK®2 automated system. VITEK ®2 indicated agreement in 19 (79.17%) isolates and difference in five (20.83%) isolates when compared to results from conventional biochemical tests. Further, antibiotic susceptibility profile results showed that all isolates (100%) were resistant to at least one out of the 18 antibiotics tested by VITEK®2. Thus, no multi-resistant bacteria that may be used in effluent treatment systems or in bioremediation processes have been reported. Results indicate VITEK ® 2 automated system as a potential methodology in the determination of susceptibility profile and identification of environmental bacteria.

Sinergicity test of silver nanoparticles and clindamycin against Staphylococcus aureus

“…The Vitek is an automated microbiology system, and it can be used to identify microorganisms. The Vitek system is a useful tool to identify bacteria by miniaturized biochemical tests with special cards containing 64 luorescent biochemical tests (Rave et al, 2018). The carbohydrate fermentation test is one of the biochemical tests to identify a microorganism.…”

Ascorbic acid inhibitory activity on resistant strains of Enterobacter spp.: in vitro study

“…according to routine biochemical testing. 13 After confirmation of isolates, they were collected on glycerol broth and stored at-80 freezer until been used.…”

Isolation and Evaluation of Clinically Important Acinetobacter Baumannii From Intensive Care Unit Samples

“…Surprisingly, hospital furniture such as patients' mattresses, bed railings, curtains, stethoscopes, computers, and telephones were the main sources of A. baumannii infection, where this bacterium was isolated [4]. Therefore, there is an extreme need to prevent A. baumannii infections especially in ICU by creating new strategies and managements for isolation [5]. Acinetobacter species caused many diseases such as pneumonia, bacteremia, UTIs, skin and soft tissue infections leading to high escalation of morbidity and mortality [6].…”

“…Consequently, there is a necessary need to create a connection between biofilm formation and antibiotics resistance in isolates of A. baumannii [4]. Conventional detections were used for early diagnosis to distinguish the infections of this organism in the hospitals, which considered as a routine cultivation methods [5][6]. This bacterium can be transmitted through exposure to aerosol droplets, hands touching from person-to-person, sputum, urine, faces or via surfaces contaminated with fomite in the hospitals [7].…”