Antiparasitic drugs: in vitro tests against nematophagous fungi

Abstract

The use of biological agents has been intensified in recent years against eggs and larvae of gastrointestinal nematodes as an alternative control method in pasture plant health management, with the concomitant use with antiparasitic drugs still occurring. The aim of this study was to test the in vitro activity of the following antiparasitic drugs: Ivermectin and albendazole against the following nematophagous fungi: Paecilomyces fumosoroseus, Paecilomyces lilacinus and Paecilomyces variotii. The agar diffusion test was performed using an initial concentration of 0.0016g/mL of each drug, after solidification of the culture medium containing the drug concentration each nematophagous fungi was inoculated. The results showed that in a concentration of 80μg/mL, the fungal growth decreased, however, with the concentration of 160μg/mL, there was no fungal growth in both drugs, compared to the control, which indicates an inhibition in the development of the nematophagous fungi studied when they come in contact with ivermectin and albendazole.Keywords: ivermectin, albendazole, biological control. Antiparasitários: teste in vitro contra fungos nematófagosResumo O uso de agentes biológicos que atuam em ovos e larvas de nematódeos gastrintestinais como uma alternativa para o manejo de pastagens de saúde tem se intensificado nos últimos anos, bem como o uso concomitante com outros medicamentos antiparasitários. O objetivo deste estudo foi testar o efeito in vitro dos fármacos Ivermectina e Albendazol em fungos nematófagos Paecilomyces fumosoroseus, Paecilomyces lilacinus e Paecilomyces variotii. Foi utilizada a técnica de difusão em agar, sendo preparado a partir de uma concentração inicial de 0,0016g/mL de cada uma das drogas e diluídas em meio de cultura, com posterior semeadura dos fungos nematófagos. Os resultados mostraram que na concentração de 80μg/mL, o crescimento diminuiu, no entanto, com a concentração de 160μg/mL de ambas as drogas, não houve crescimento de fungos durante o período de estudo, em comparação com o controle, indicando a inibição do desenvolvimento dos fungos nematófagos estudados quando em contato com a Ivermectina e Albendazol.Palavras-chave: ivermectina, albendazol, controle biológico.

Nematophagous fungi and their influences on parasitic control in sheep

“…The supply of isolates of predatory fungi is often not as simplified as it is related, acting time is varied, called the "grace period" because, at this time, specific conditions are needed for the activation and performance of nematophagous adequately (Carneiro, 1992). Studies have demonstrated the intervention of some variables in the maturation of fungi, which may become considerable problems over time (Vieira, 2017, Ferreira et al, 2016, Serra et al, 2017 Research, Society and Development, v. 12, n. 4, e21512441126, 2023 (CC BY 4.0) | ISSN 2525-3409 | DOI: http://dx.doi.org/10.33448/rsd-v12i4.41126 9…”

Asociación in vitro de Duddingtonia flagrans con ivermectina en el control de nematodos gastrointestinales de búfalos

“…Como se mencionó anteriormente, algunos estudios recientes han demostrado que la asociación de estos hongos con fármacos antihelmínticos ha tenido éxito en la reducción de las formas larvarias de los nematodos parásitos y, desde un punto de vista sostenible, puede convertirse en una estrategia futura (11,23,24). Sin embargo, se entiende que el uso de algún fármaco antihelmíntico puede inhibir el desarrollo de los hongos, comprometiendo su eficacia en el control biológico (25,26). Por lo tanto, los experimentos in vitro son necesarios para probar o no la acción sinérgica de la asociación química y biológica, como se destaca aquí.…”

10. Public-private partnership enabled use of anti-tick vaccine for integrated cattle fever tick eradication in the USA

“…There have been many studies on this issue and this chapter cites only the most recent ones in order to demonstrate that the environmental impact of acaricides is a persisting, if not increasing problem. While acaricides can act in synergy with entomopathogens, as shown by several studies (Bahiense and Bittencourt 2004;Farenhorst et al 2010), they may also negatively affect the very entomopathogens that are desirable to control the cattle tick (Ferreira et al 2016;Singh et al 2010;Vieira et al 2016). In addition, acaricides have to the potential to induce dysbioses in hosts (Nasuti et al 2016) and possibly modify the microbial composition of soil (Qi and Wei 2017), with other negative consequences for many aspects of cattle and/or environmental health besides the latter's entomofauna.…”