Antifungal susceptibility profile of diferent yeasts isolates from wild animals, cow’s milk with subclinical mastitis and hospital environment

Abstract

Yeast infections have acquired great importance due to increasing frequency in immunocompromised patients or patients undergoing invasive diagnostic and therapeutic techniques, and also because of its high morbidity and mortality. At the same time, it has been seen an increase in the emergence of new pathogenic species difficult to diagnose and treat. The aim of this study was to determine the in vitro susceptibility of 89 yeasts from different sources against the antifungals amphotericin B, voriconazole, fluconazole and flucytosine, using the VITEK  2 Compact system. The antifungal susceptibility was performed automatically by the Vitek  2 Compact system. The origin of the yeasts was: Group 1 -microbiota of wild animals (W) (26/89), 2 -cow's milk with subclinical mastitis (M) (27/89) and 3 -hospital enviorment (H) (36/89). Of the 89 yeasts submitted to the Vitek  2 test, 25 (20.9%) were resistant to fluconazole, 11 (12.36%) to amphotericin B, 3 (3.37%) to voriconazole, and no sample was resistant to flucytosine. Regarding the minimum inhibitory concentration (MIC), fluconazole showed an MIC between 1 and 64 mg/mL for the three groups, voriconazole had an MIC between 0.12 and 8 mg/mL, amphotericin B had an MIC between 0.25 and 4 mg/mL for group H and group W respectively, between 0.25 and 16 mg/mL for group M and flucytosine had an MIC equal to 1μg/mL for all groups. The yeasts isolated from the H group showed the highest resistance to fluconazole 12/89 (13.49%), followed by group W (7.87%) and group M (5.62%). The more resistant group to voriconazole was followed by the M and H groups, the W group showed no resistance to this antifungal. Group H was the least resistant (2.25%) to amphotericin.

Identification and antimicrobial suceptibility profile of bacteria causing bovine mastitis from dairy farms in Pelotas, Rio Grande do Sul

“…isolates were selected. The confirmation of the species was given trough the Vitek® 2 Compact system, as reported in the work of Mendes et al (2016), adapted for bacteria. For the microorganisms' susceptibility profile determination, the Kirb-Bauer disk diffusion technique (Brasil, 2003), was used.…”

Klonska raznolikost i antifungalna osjetljivost vrste Candida spp. izolirane iz mlijeka

“…In this study, antimicrobial resistance was de- The Vitek ® MS -Mass spectrometry (bioMérieux) is used for in vitro diagnosis and microbial identification a unique and first American approved and FDA certified mass spectrometer (Mendes et al, 2018). The Vitek ® 2 Compact system is commonly utilized by researchers to identify microorganisms and is a practical and efficient machine (Melhem et al, 2014;Zhang et al, 2014;Mendes et al, 2018). In the Rep-PCR fingerprinting stage of the study, one of 37 isolates could not be cultured.…”

Prevalence of Virulence Genes and Antifungal Resistance in Candida albicans Isolated from Raw Goat Milk

“…The existence of virulence genes and the elevation in the prevalence of resistance against antifungal agents have been incriminated in the pathogenesis of Candida albicans. The antifungal resistance is increasing due to the use of inadequate doses of the selective therapies as well as the frequent use of antifungal agents for fungal infection prophylaxis in both humans and animals (Mendes et al, 2018). However, the relationship between virulence genes and the resistance profiles of Candida albicans has not been sufficiently investigated, particularly in deep Candida albicans infections (Shrief et al, 2019).…”