Angiogenic activity of latex from Euphorbia tirucalliLinnaeus 1753 (Plantae, Euphorbiaceae)

Abstract

To assess the pro-angiogenic activity of Euphorbia tirucalli, commonly known as "avelós" plant, we performed a series of tests by applying an aqueous E. tirucalli latex solution (10 mg/mL) to the chorioallantoic membranes (CAMs) of 80 fertilized chicken eggs incubated in a temperature-and humidity-controlled automatic incubator. The results indicated that the aqueous latex solution increased vascular network formation compared to that with the negative control (p < 0.05) and the inhibitor control (p < 0.05). This suggests that under the experimental conditions tested, the aqueous latex solution induced an inflammatory response leading to neoangiogenesis.Keywords: angiogenesis, chorioallantoic membrane, Euphorbia tirucalli. Atividade angiogênica do látex da Euphorbia tirucalliLinnaeus 1753 (Plantae, Euphorbiaceae) ResumoCom o objetivo de analisar a atividade angiogênica apresentada pela Euphorbia tirucalli, popularmente conhecida comumente como "avelós", foram realizados ensaios utilizando solução aquosa de látex na concentração de 10 mg/ml, aplicada em membrana corioalantóide (MCA) de 80 ovos férteis de galinha, incubado em estufa automática com temperatura e umidade controladas. Os resultados apontaram que a ação da solução aquosa provocou aumento da percentagem da rede vascular formada em relação aos controles negativo (p<0,05) e inibidor (p<0,05), indicando que nas condições deste experimento, foi responsável pela ativação da resposta inflamatória e crescimento de novos vasos sanguíneos.Palavras-chave: angiogênese, membrana corioalantóide, Euphorbia tirucalli.

Euphorbia species latex: A comprehensive review on phytochemistry and biological activities

“…These data suggest that these cancer cells were strongly affected by the phenolic compounds detected in the latex extract (Abdel-Aty et al, 2019). In another study of the same species; the crude latex extract of E. tirucalli reduced the viability of gastric adenocarcinoma cancer cells at concentrations of 100 (Bessa et al, 2015).…”

“…The positive control and E. tirucalli latex groups showed an increase in the number of blood vessels and an inflammatory response, whereas few blood vessels were found in the control group treated with 1% dexamethasone. Thus, the latex of E. tirucalli led to the activation of the inflammatory response ( Bessa et al, 2015 ). On the other hand, the anti-angiogenic activity of E. helioscopia latex (100 μg/mL) has been studied in fertilized white leghorn hen eggs.…”

“…The inducer control (Biocure Biomembrane of Hevea brasiliensis latex) treatment induced a large number of blood vessels and inflammatory foci (C,D) . Treatment with the test solution of Euphorbia tirucalli latex resulted in a large number of well-organized blood vessels and inflammatory foci (E,F) ( Bessa et al, 2015 ).…”

“…FIGURE 17 Photomicrography of Chorioallantoic membrane (CAM) vascular network formation. (A) Inhibitor dexamethasone; (B) the negative control (water); (C) the test solution (E. tirucalli); and (D) the inducer control (Biocure Biomembrane)(Bessa et al, 2015).…”

Effect of crude latex from Euphorbia tirucalli on DMBA-induced carcinogenesis

“…The latex from this species is commonly used in traditional medicine [3] for the treatment of cancer [2,[4][5][6][7]. This substance is also reported to have immunomodulatory [7,8], analgesic [9], angiogenic [10], antimicrobial [11], antimutagenic and antirheumatic [12] properties.…”