Sperm quality of the Amazon catfish Leiarius marmoratus (Gill, 1870) after cold storage

Galo, JM; Dp, Streit; Povh, Jayme Aparecido; Fornari, Darci Carlos; Resende, E. K. de; Oliveira, Diogo Losh de; Rp, Ribeiro

doi:10.1590/1519-6984.00313

Cited by 5 publications

(11 citation statements)

References 16 publications

(11 reference statements)

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“…A concentração espermática ficou bem próxima dos valores observados por Araújo et al (2014), 3,13 x 10 9 mL -1 , e Navarro e Pivato (2016), 1,79 x 10 9 mL -1 , e dentro da faixa mais ampla, 1,87-6,30 x 10 9 mL -1 , registrada por Mira-López et al (2010). A motilidade espermática observada no sêmen fresco de 84% foi semelhante à maioria dos relatos (Mira Lopez et al, 2010;Galo et al, 2014;Navarro e Pivato, 2016). Quanto à duração da motilidade, Mira-Lopez et al (2010), Araújo et al (2014) e Galo et al (2014) observaram valores menores, entre 52 e 110 segundos, enquanto Navarro e Pivato (2016) registraram valores médios de 318 segundos.…”

Section: Resultsunclassified

“…A criopreservação de sêmen de peixes é uma importante ferramenta tanto para o desenvolvimento de programas de melhoramento genético como para a conservação de recursos genéticos em bancos de germoplasma (Carosfeld et al, 2003;Martínez-Páramo et al, 2017). Existem poucos estudos sobre as características morfológicas do sêmen e sua conservação na espécie (Mira-Lopez et al, 2010;Araújo et al, 2014;Galo et al, 2014;Navarro e Pivato, 2016). No jundiá amazônico, esse é o primeiro relato da criopreservação de sêmen e das características ultraestruturais do espermatozoide, aspectos relevantes que podem contribuir para a definição de protocolos e de padronização das etapas essenciais para que a técnica seja acessível em escala nas fazendas de criação de peixes.…”

Section: Introductionunclassified

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Ultraestrutura e criopreservação de sêmen de jundiá amazônico (Leiarius marmoratus) em cativeiro

Borges

Araújo

Pivato

et al. 2020

Arq. Bras. Med. Vet. Zootec.

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RESUMO Os objetivos do presente estudo foram analisar a ultraestrutura do espermatozoide do jundiá amazônico e avaliar a sua criopreservação com três agentes crioprotetores (metanol 10%, DMSO 10% e etilenoglicol 10%) e duas soluções ativadoras (NaCl 0,29% e NaHCO3 1%). Como diluente, foi utilizada uma solução de glicose a 5%, sendo o sêmen envasado em palhetas de 0,25mL e congelado em vapor de nitrogênio (botijão dry shipper). No sêmen fresco, o espermatozoide apresentou comprimento de 25,46±2,54μm, cabeça esférica (1,51±0,18μm), ausência de acrossoma, peça intermediária com formato cônico (0,93±0,17μm), ligeiramente assimétrica, com presença de vesículas, e flagelo único (21,48±2,45μm). O sêmen descongelado apresentou valores mais altos (P<0,05) para duração, vigor e taxa de motilidade espermática com os crioprotetores metanol 10% e DMSO 10%. A duração da motilidade espermática foi maior (P<0,05) com o ativador NaHCO3 1% (21-96 s). O sêmen de Leiarius marmoratus criopreservado com DMSO e metanol apresentou, respectivamente, 7,32±4,21% e 8,94±6,69% de taxa de motilidade. No entanto, os resultados não foram satisfatórios para estabelecer um protocolo para a espécie.

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Section: Resultsunclassified

Section: Introductionunclassified

Ultraestrutura e criopreservação de sêmen de jundiá amazônico (Leiarius marmoratus) em cativeiro

Borges

Araújo

Pivato

et al. 2020

Arq. Bras. Med. Vet. Zootec.

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“…There are no studies on cooling of C. macropomum semen. However, the retention of sperm MOT by cooling has been reported for the tropical species L. friderici and L. elongatus (30% at 8 hr), P. mesopotamicus and P. lineatus (40% at 20 hr) (Marques & Godinho, ) and L. marmoratus (40% at 7 hr) (Galo et al, ). Compared to the results reported in these studies, MOT retention was higher in the present study (33.72% at 25 hr).…”

Section: Discussionmentioning

confidence: 96%

“…Hypothermic storage of semen is an efficient technique that can be used in the reproductive management of fish due to its lower costs compared with freezing techniques (He & Woods, 2003). Recent studies on tropical species such as Leporinus friderici, Leporinus elongatus, Piaractus mesopotamicus, Prochilodus lineatus (Marques & Godinho, 2004) and Leiarius marmoratus (Galo et al, 2014) described positive results of using semen cooling techniques.…”

Section: Introductionmentioning

confidence: 99%

Sperm quality of Colossoma macropomum after room‐temperature and cold storage

Pires

Sanches

Romagosa³

et al. 2019

J Appl Ichthyol

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Summary The objective of this study was to evaluate the effects of cold and room‐temperature storage on the quality of Colossoma macropomum sperm. The experiment was carried out in December (end of Spring), in Nova Mutum‐MT, Brazil, involving nine C. macropomum males (4 years old; 6.4 ± 1.5 kg average weight). The fish were selected and transferred to masonry tanks (4 m3) in a laboratory (water renewal rate: 10 L/s; average water temperature: 28°C). Subsequently, reproduction was induced using 2.5 mg of crude carp pituitary extract/kg and the semen was harvested 240 degree hours after hormonal induction. The following sperm characteristics were analyzed every 5 hr using ImageJ/casa software: total motility (MOT), curvilinear velocity (VCL), average path velocity (VAP), straight‐line velocity (VSL), straightness of sperm path (STR), wobble (WOB), progressive motility (PROG), beat cross frequency (BCF) and total number of spermatozoa (NSPZ). A fresh sample of semen from each animal was kept at room temperature (25.3 ± 1.2°C). For analysis of cooled semen, syringes were kept in cooling boxes at an average temperature of 16.9 ± 2.1°C. The reduction (p < 0.05) of MOT in semen kept at room temperature occurred at 10 hr (13.95%); in cooled semen, however, MOT declined at 15 hr (76.87%). At 15 hr, there was practically no MOT in the semen kept at room temperature (0.20%), whereas in the cooled semen this situation was observed only at 35 hr (2.91%) The MOT of cooled sperm was higher (p < 0.05) at all times (except zero time), compared with the semen maintained at room temperature. At 15 hr, the cooled spermatozoa showed higher (p < 0.05) VCL (142.18 μm/s) and BCF (29.72 Hz) than those maintained at room temperature (VCL: 51.18 μm/s; BCF: 19.57 Hz). After 15 hr, only the cooled sperm showed quality. In conclusion, semen cooling allows for extending the viability of C. macropomum spermatozoa from 5 to 10 hr without compromising their quality in most characteristics. At 15 and 25 hr of cooling, sperm viability is still observed, though with decreased quality.

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“…Semen quality and other factors such as the water used for gamete activation are important to optimize in vitro fertilization. Studies evaluating the semen characteristics of South American fishes, such as Steindachneridion scripta (Luz et al, 2001), Leporinus elongatus (Streit Junior et al, 2008), Colossoma macropomum (Maria et al, 2012), Brycon orbignyanus (Galo et al, 2011), Pseudoplatystoma reticulatum (Streit Junior et al, 2012), Leiarius marmoratus (Galo et al, 2014) and S. melanodermatum (Marcos et al, 2015) have been conducted in recent years to determinate the actual potential of each species in fish farming activities.…”

Section: Introductionmentioning

confidence: 99%

Quantitative and qualitative variables of semen from surubim do Iguaçu, Steindachneridion melanodermatum Garavello, 2005 (Siluriformes: Pimelodidae)

et al. 2019

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The objective of the present work was to assess the qualitative and quantitative characteristics of semen from the surubim do Iguaçu (Steindachneridion melanodermatum). Induced spermiation was achieved in eleven males with mean weight of 1.76 ± 0.48 kg and average age of two years and semen was collected by stripping. The average volume was 1.34 ± 0.73 mL. The duration of sperm motility was 154.4 ± 72.6 and 149.0 ± 77.5 seconds after activation with hatchery water and distilled water, respectively. The sperm concentration estimated by hemocytometer was 5.423 ± 2.155 x 1010 spermatozoa/mL. The results indicate that S. melanodermatum semen is easily obtained during the spawning season and the seminal characteristics are adequate insemination and subsequent in vitro fertilization.

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Sperm quality of the Amazon catfish Leiarius marmoratus (Gill, 1870) after cold storage

Cited by 5 publications

References 16 publications

Ultraestrutura e criopreservação de sêmen de jundiá amazônico (Leiarius marmoratus) em cativeiro

Ultraestrutura e criopreservação de sêmen de jundiá amazônico (Leiarius marmoratus) em cativeiro

Sperm quality of Colossoma macropomum after room‐temperature and cold storage

Quantitative and qualitative variables of semen from surubim do Iguaçu, Steindachneridion melanodermatum Garavello, 2005 (Siluriformes: Pimelodidae)

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