Campylobacter Jejuni Increases Transcribed Il-1 B and Causes Morphometric Changes in the Ileal Enterocytes of Chickens

Fonseca, Belchiolina Beatriz; Júnior, Álvaro Ferreira; Santos, JP dos; Coelho, LR; Rossi, D. A.; Melo, RT; Mendonça, EP; Araújo, TG; Alves, Rosiane Nascimento; Beletti, Marcelo Emı́lio

doi:10.1590/1516-635x1801063-068

Cited by 3 publications

(2 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was described that children and pets with CJ infections were resistant to ceftiofur, sulphazotrim, norfloxacin, tetracycline, amoxicillin, ciprofloxacin, cefazolin, and erythromycin antibiotics ( Rodrigues et al., 2015 ). Furthermore, another work showed that CJ isolated from chicken carcasses were highly resistant to norfloxacin, erythromycin, and amoxicillin, the most used drugs in traditional antibiotic therapy ( Fonseca et al., 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Nanocarriers From Natural Lipids With In Vitro Activity Against Campylobacter jejuni

Ribeiro

Paula

Rossi

et al. 2021

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Campylobacter jejuni (CJ) is the most prevalent zoonotic pathogen of chicken meat and related products, which may lead to gastroenteritis and autoimmune diseases in humans. Although controlling this bacterium is important, CJ strains resistance against traditional antibiotic therapy has been increased. Vegetable oils and fats are natural biomaterials explored since the Ancient times, due to their therapeutic properties. Nanotechnology has promoted the miniaturization of materials, improving bioavailability and efficacy, while reducing the toxicity of loaded active molecules. In this work, a screening of 28 vegetable oils was firstly performed, in order to select anti-CJ candidates by the disc diffusion test. Thus, the selected liquid lipids were used as active molecules in nanostructured lipid carriers (NLC) formulations. The three resultant systems were characterized in terms of particle size (~200 nm), polydispersity index (~0.15), and zeta potential (~-35mV), and its physicochemical stability was confirmed for a year, at 25°C. The structural properties of NLC were assessed by infrared (FTIR-ATR) and differential scanning calorimetry (DSC) analyses. The spherical nanoparticle morphology and narrow size distribution was observed by transmission electron microscopy (TEM) and field emission scanning electron (FE-SEM) analyses, respectively. Then, the in vitro antimicrobial activity test determined the minimum inhibitory concentration (MIC) of each formulation against CJ strains, in both free (1–3 mg/ml−1) and sessile (0.78 mg/ml−1) forms. Finally, the in vitro biocompatibility of NLC was demonstrated through cell viability using VERO cell line, in which F6 was found twice less cytotoxic than pure olibanum oil. Considering the abovementioned achieved, F6 formulation is able to be evaluated in the in vivo anti-CJ efficacy assays.

show abstract

Section: Introductionmentioning

confidence: 99%

Nanocarriers From Natural Lipids With In Vitro Activity Against Campylobacter jejuni

Ribeiro

Paula

Rossi

et al. 2021

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

show abstract

“…It is involved in various cellular activities such as cell proliferation, differentiation and apoptosis (Palomo et al, 2015). C. jejuni increases IL-1β gene transcription in chickens, with elevated levels of IL-1β, -6 and -8 transcription in chicken cells infected with C. jejuni having the ability to activate innate immunity signalling pathways in chicken ileum explants (Fonseca et al, 2016;Li et al, 2008;Smith et al, 2005).…”

Section: Immune Biomarkers In Chickensmentioning

confidence: 99%

Host Pathogen responses of chicken cells in vitro and in vivo to a diverse population of Campylobacter strains

John¹

View full text Add to dashboard Cite

Campylobacter is the leading cause of bacterial food-borne diarrheal disease worldwide, with the bacteria being pathogens in humans and chickens. There are differences in the mechanistic understanding between Campylobacter strains, due, in part, to genomic and phenotypic strain diversity, which leads to inconsistent findings. This study aimed to understand underlying infection biology in host-pathogen interactions between populations of Campylobacter and the chicken host. A collection of Campylobacter jejuni strains were used to investigate the genotypic and phenotypic differences which yielded a diverse pan-geome of 2715 genes with genome wide association studies uncovering genes related to flagella assocaited with strains isolated from both humans and chickens. The C. jejuni strains were then used with a new commercially available chicken cell line to investigate the chicken systems with host responses measured in comparison to human systems. The pathogenic diversity of C. jejuni was measured through their inflammatory, cytotoxicity, adhesion, invasion and signalling responses in a high-throughput model using avian and human intestinal epithelial cells. C. jejuni induced IL-8 and CXCLi1/2 in human and avian epithelial cells, respectively, in a MAP kinase-dependent manner. In contrast, IL-10 responses in both cell types were PI 3kinase/Akt-dependent. C. jejuni strains showed diverse levels of invasion with high invasion dependent on MAP kinase signaling in both cell lines. C. jejuni induced diverse cytotoxic responses in both cell lines with cdt-positive isolates showing significantly higher toxicity. Blockade of endocytic pathways suggested that invasion by C. jejuni was clathrin-and dynamin-dependent but caveolae-independent in both cells. In contrast, IL-8 (and CXCLi1/2) production was dependent on clathrin, dynamin, and caveolae. Strong correlations were found between IL-8 with invasion and toxicity responses with GWAS being able to associated genes to expression of IL-8,-10 and level of invasion. Campylobacter strains were isolated from the caeca, ileum and liver and a pan-genome created with GWAS analysis discovering genes relating to iron transport and heat shock proteins in strains from the liver. Subsequently, caecal tonsils were extracted from chickens uninfected and chickens infected with a cocktail of six Campylobacter strains with a Campylobacter effect found where infected chickens produced significantly higher T h 1, T h 2 and T h 17 cytokines. The work in this thesis contributes invaluable knowledge on the chicken innate immune system in response to Campylobacter and how similar host defence pathways to humans are utlised. The diversity found strongly indicates that a "one strain fits all" approach to in vitro and in vivo experimental infections may not give accurate results on Campylobacter pathogenesis. Campylobacter isolates can use host immune responses to its advantage and to move from the gut of chickens to infect edible tissues such as the liver.

show abstract

Colonization of Campylobacter jejuni in Poultry

Batistão

Fonseca

Júnior

et al. 2016

Campylobacter Spp. And Related Organisms in Poultry

View full text Add to dashboard Cite

Campylobacter Jejuni Increases Transcribed Il-1 B and Causes Morphometric Changes in the Ileal Enterocytes of Chickens

Cited by 3 publications

References 27 publications

Nanocarriers From Natural Lipids With In Vitro Activity Against Campylobacter jejuni

Nanocarriers From Natural Lipids With In Vitro Activity Against Campylobacter jejuni

Host Pathogen responses of chicken cells in vitro and in vivo to a diverse population of Campylobacter strains

Colonization of Campylobacter jejuni in Poultry

Contact Info

Product

Resources

About