Single Nucleotide Polymorphisms in IGFBP-2 Gene and Their Associations with Body Weight Traits on Jinghai Yellow Chicken

Abstract

Insulin-like growth factor binding protein-2 (IGFBP-2) regulates a broad spectrum of biological activities involved in growth, development, and differentiation. This study aimed at comparing polymorphisms in intron2 of the IGFBP-2 gene among four chicken breeds and at analyzing the associations between its genotypes and body weight in Jinghai Yellow chicken by using PCR-SSCP technique. For primer P2, three genotypes (AA, AB and BB) were observed in the four chicken breeds. Gene sequencing revealed one insertion/deletion (the inserted/ deleted TC after position 552bp) in the intron 2 of IGFBP-2 gene. For primer P5, three genotypes were identified in Jinghai Yellow chickens, and named CC, CD and DD. Gene sequencing revealed two SNPs (C1107G, C1130T) and one inserted/deleted GCCAGGT after 1115bp in the intron 2 of IGFBP-2 gene. The results of the linear model analysis showed that Jinghai Yellow chickens with AA genotype had significantly heavier body weight, at hatch and 12 weeks of age, than those of the AB genotype (p<0.05). The A allele had a positive effect on body weight. We speculate that mutations in intron 2 could be used as genetic markers for body weight in Jinghai Yellow chicken. This study provides valuable information for the protection of genetic resources and for breeding of Jinghai Yellow chicken.

Genetic Analysis of SNPs in GH, GHR, IGF-I and IGFBPII Genes and their Association with Some Productive and Reproductive Traits in Native Breeder Hens

“…In another study carried out by these researchers, intron 2 region of IGFBPII gene was studied in this population and three genotypes of AA, AB and BB (equivalent to CC, CT and TT in the Mazandaran native fowls population) were detected and no significant correlation was found between genotypes and body weight at 4, 8, 12 and 16 weeks of age. Also, mean comparisons analysis indicated that AA genotypes had higher body weight at 12 weeks of age than other genotypes and it was concluded that the A allele had a positive effect on body weight [24]. In the present study, three genotypes of CC, CT and TT were found with the frequency of 0.14, 0.62 and 0.24, respectively, in Mazandaran native fowls population, and the genotypic frequency of CT was significantly higher than the other two genotypes.…”

“…The insulin-like growth factor binding protein II gene (IGFBPII) has the size of approximately 38 kbp that is located on chromosome 7, entailing four short exons and three long introns and also contains 275 amino acids [22]. In indigenous and commercial chicken populations, significant associations of this gene were reported with body weight at the time of hatch and days 7, 14, 21, and 28 of age [22], growth rate at 4-8 and 8-12 weeks of age in hens and 0-4 weeks of age in rosters [23], and body weight at the time of hatch and 12 weeks of age [24]. Moreover, some SNPs have been detected to have significant effect on growth traits in indigenous chickens such as a SNP (C/T) in intron 2 on body weight at 2-12 weeks of age, length and weight of metatartos, shank, femur regions and abdominal fat weight [25], and also a SNP (1196CA) in 3'-flanking region on abdominal fat mass and its percentage [26].…”

Exploring the Potential and Limitations of PCR-RFLP and PCR-SSCP for SNP Detection: A Review

“…57 However, PCR-SSCP was considered as a cornerstone in recent post-PCR genotyping studies on poultry, such as egg-production traits, body weight characteristics, and intramuscular fat content. [58][59][60] Because of its powerful ability to identify unknown SNP(s), Table 1. The Main Differences Between PCR-RFLP and PCR-SSCP Methods…”

“…[62][63][64] With respect to fish and amphibians, species identification and the detection of environmental samples and closely related organisms were recently conducted using PCR-RFLP. [56][57][58][59][60][61][62][63][64][65][66][67][68] The PCR-SSCP was used in the detection of parasite species. 69 The ease of the use of PCR-RFLP was demonstrated in its successful implementation in species identification and discrimination in algae, bacteria, and fungi.…”

A Polymorphism of Insulin-Like Growth Factor Binding Protein 2 Gene Associated with Growth and Body Composition Traits in Kampong Chickens

“…Previous studies have shown that some Single Nucleotide Polymorphisms (SNP) of IGFBP2 significantly affected growth traits (Lei et al, 2005;Sri-Sudaryati et al, 2013;Zhao et al, 2015). Leng et al (2009) reported that a polymorphism of IGFBP2 in the 3;-flanking region was associated with abdominal fat.…”