4'-O-β-D-glucosyl-5-O-methylvisamminol ameliorates imiquimod-induced psoriasis-like dermatitis and inhibits inflammatory cytokines production by suppressing the NF-κB and MAPK signaling pathways

Abstract

Psoriasis is a chronic inflammatory skin disorder in humans, and the inflammatory reaction plays an important role in development and onset of psoriasis. 4'-O-b-D-glucosyl-5-O-methylvisamminol (4GMV) is one of the major active chromones isolated from Saposhnikoviae divaricata (Turcz.) Schischk, which has been reported to exhibit excellent anti-inflammatory activities. However, the possible therapeutic effect on psoriasis and underlying mechanism has not been reported. Thus, the aim of this study was to investigate the protective effect of 4GMV on the imiquimod (IMQ)-induced psoriasis-like lesions in BALB/c mice and the anti-inflammatory effect on the lipopolysaccharide (LPS)-induced inflammation in RAW264.7 macrophages. The results demonstrated that 4GMV decreased IMQ-induced keratinocyte proliferation and inflammatory cell infiltration. Moreover, 4GMV treatment significantly inhibited the production of NO, PEG 2, and cytokines such as interleukin (IL)-1b, IL-6, interferon (IFN)-g, and IL-22 in LPS-stimulated RAW264.7 macrophages. 4GMV also suppressed the LPSupregulated protein expressions of iNOS and COX-2 in a dose-dependent manner. Furthermore, qRT-PCR analysis showed that 4GMV down-regulated the mRNA level of IL-1b and IL-6 expression. Further studies by western blot indicated that 4GMV inhibited the activation of upstream mediator NF-kB by suppressing the expression of TLR4 and the phosphorylation of IkBa and p65. The phosphorylation of JNK, p38, and ERK were also markedly reversed by 4GMV in LPS-treated RAW264.7 macrophages. Taken together, these results demonstrated that 4GMV showed a protective effect in IMQ-induced psoriasis-like mice and inhibited inflammation through the NF-kB and MAPK signaling pathways, indicating that 4GMV might be a potential therapeutic drug for psoriasis.

Molecular Diversity and Biochemical Content in Two Invasive Alien Species: Looking for Chemical Similarities and Bioactivities

“…In A. armata ( Table 2 ), three molecules (two chromones and one trihydroxyoctadecenoic acid) with anti-inflammatory activity and two with analgesic properties were found in d H2O and d H 2 O:MeOH(1:4) [ 52 , 53 , 54 ]. Multiple other health benefits were observed in the formula C 22 H 28 O 11 in the form of chromones [ 55 , 56 , 57 , 58 ].…”

Possible Mechanisms of Oxidative Stress-Induced Skin Cellular Senescence, Inflammation, and Cancer and the Therapeutic Potential of Plant Polyphenols

“…Thus, it regulates immune responses, participates in intracellular signal transduction, and regulates tissue cell pathology and physiology [ 69 ]. Various cytokines and adhesion molecules, including interleukin (IL)-1, IL-2, IL-6, IL-8, IL-12, IL-18, TNF-α, and nerve growth factor, are abnormally expressed in the lesions of psoriasis patients, suggesting that psoriasis is associated with the release of NF-κB-regulated inflammatory cytokines [ 70 ]. NF-κB is present in the promoters or enhancers of genes encoding these factors.…”

Transcriptomics explores the potential of flavonoid in non-medicinal parts of Saposhnikovia divaricata (Turcz.) Schischk

“…Prim-Oglucosylcimifugin, 4'-O-b-D-glucosyl-5-O-methylvisamminol, cimifugin, and sec-O-glucosylhamaudol are widely considered as active compounds in S. divaricata due to their high content and strong pharmacological activities. Although their oral bioavailability is less than 30%, they are considered as bioactive compounds and can be used for further analysis (Jia et al, 2016;Kim et al, 2017;Zhou et al, 2017;Fu et al, 2020;Li et al, 2020). Thus, a total of 22 compounds were obtained, as shown in Table 3.…”

“…They are the primary active components of S. divaricata, and research on these compounds has been well studied than that on other components. Studies have shown that the active components in prim-O-glucosylcimifugin and 4'-O-b-D-glucosyl-5-O-methylvisamminol can not only effectively inhibit a variety of stimuli caused by pain in mice, but also can effectively reduce the xylene-induced ear swelling in mice (Zhou et al, 2017;Fu et al, 2020). Cimifugin has good antipyretic, analgesic and anti-inflammatory effects (Jia et al, 2016).…”