Inhibition of miR-383 suppresses oxidative stress and improves endothelial function by increasing sirtuin 1

Hu, Baoxiang; Gong, Zushun; Bi, Zhaohui

doi:10.1590/1414-431x20198616

Cited by 10 publications

(6 citation statements)

References 35 publications

(34 reference statements)

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“…MiR-92a inhibition reduces oxidative stress and improves endothelial function by upregulating the expression of HO-1 [ 95 ]. Inhibition of miR-383 reduced ROS by upregulating SIRT1, increasing SOD1/CAT levels, and enhancing endothelial cell activity [ 96 ].…”

Section: Mechanisms Of Ros-induced Endothelial Dysfunction In Diabeti...mentioning

confidence: 99%

The role of oxidative stress in diabetes mellitus-induced vascular endothelial dysfunction

An,

Xu,

Wan

et al. 2023

Cardiovasc Diabetol

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Diabetes mellitus is a metabolic disease characterized by long-term hyperglycaemia, which leads to microangiopathy and macroangiopathy and ultimately increases the mortality of diabetic patients. Endothelial dysfunction, which has been recognized as a key factor in the pathogenesis of diabetic microangiopathy and macroangiopathy, is characterized by a reduction in NO bioavailability. Oxidative stress, which is the main pathogenic factor in diabetes, is one of the major triggers of endothelial dysfunction through the reduction in NO. In this review, we summarize the four sources of ROS in the diabetic vasculature and the underlying molecular mechanisms by which the pathogenic factors hyperglycaemia, hyperlipidaemia, adipokines and insulin resistance induce oxidative stress in endothelial cells in the context of diabetes. In addition, we discuss oxidative stress-targeted interventions, including hypoglycaemic drugs, antioxidants and lifestyle interventions, and their effects on diabetes-induced endothelial dysfunction. In summary, our review provides comprehensive insight into the roles of oxidative stress in diabetes-induced endothelial dysfunction.

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Section: Mechanisms Of Ros-induced Endothelial Dysfunction In Diabeti...mentioning

confidence: 99%

The role of oxidative stress in diabetes mellitus-induced vascular endothelial dysfunction

An,

Xu,

Wan

et al. 2023

Cardiovasc Diabetol

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“…Hu found that miR-383 was capable of inducing ROS generation and promote cell apoptosis [23]. Relevant studies have shown that miR-383 is associated with reproduction-related miRNA.…”

Section: Discussionmentioning

confidence: 99%

Analysis of the Resistance of Small Peptides from Periplaneta americana to Hydrogen Peroxide-induced Apoptosis in KGN Cells based on High-throughput miRNA Sequencing

Jiang,

Sun,

Tang

et al. 2023

Preprint

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Background: Apoptosis of ovarian granulosa cells can promote abnormal atresia folliculin and decrease women's reproductive function. MiRNA plays an essential molecular signal-regulating function in ovarian functional development, animal reproductive performances and follicular development. The purpose of this study is to investigate the miRNA molecules and action pathways during the resistance of small peptides from Periplaneta americana (SPPA) resistance to peroxide-induced ovarian granulosa cells (KGN cells) apoptosis. Results: The study found that 162 differentially expressed miRNAs were identified in the blank control group, H2O2 group and H2O2+SPPA group. The intersection of miRNAs between the three groups was determined, through which 13 differentially expressed miRNAs were identified. Target gene prediction of differentially expressed miRNAs yielded 4828 target genes. Finally, GO and KEGG analysis were performed on 4828 target genes. The target genes primarily engaged in transcription, DNA template, negative transcriptional regulation of RNA polymerase II promoter, negative regulation of cell proliferation, protein binding, metabolic pathways, and mitogen-activated protein kinase signalling pathways. After qRT-PCR verification, expression trends of miR-103a-3p and miR-214 were consistent with sequencing results. Conclusions: Critical miRNAs have been identified as miR-103a-3p and miR-214 in SPPA resistance to peroxide-induced KGN apoptosis.

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“…In recent years, the role of miRNA in biological physiological, and pathological processes has gradually become clear, especially in the field of chronic diseases such as diabetes. Studies have found that miRNA-34a, miRNA-24, miRNA-181c, miRNA-29b, miRNA-200a, miRNA-383, and other miRNAs play a role in inflammation ( Li, Kim et al, 2016 ; Lo, Yang et al, 2018 ; Shen, Li et al, 2018 ; Cheng, Chen et al, 2019 ; Zhang, Cai et al, 2019 ; Hu, Gong et al, 2020 ), thus participating in the occurrence of endothelial dysfunction in diabetes. To study the exact mechanism of vaccarin in regulating HDAC1, databases were used to screen the possible miRNA targets ( Figure 6A ).…”

Section: Discussionmentioning

confidence: 99%

Vaccarin alleviates endothelial inflammatory injury in diabetes by mediating miR-570-3p/HDAC1 pathway

Zhu

et al. 2022

Front. Pharmacol.

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Vaccarin is a flavonoid glycoside, which has a variety of pharmacological properties and plays a protective role in diabetes and its complications, but its mechanism is unclear. In this study, we aim to investigate whether histone deacetylase 1(HDAC1), a gene that plays a pivotal role in regulating eukaryotic gene expression, is the target of miR-570-3p in diabetic vascular endothelium, and the potential molecular mechanism of vaccarin regulating endothelial inflammatory injury through miR-570-3p/HDAC1 pathway. The HFD and streptozotocin (STZ) induced diabetes mice model, a classical type 2 diabetic model, was established. The aorta of diabetic mice displayed a decrease of miR-570-3p, the elevation of HDAC1, and inflammatory injury, which were alleviated by vaccarin. Next, we employed the role of vaccarin in regulating endothelial cells miR-570-3p and HDAC1 under hyperglycemia conditions in vitro. We discovered that overexpression of HDAC1 counteracted the inhibitory effect of vaccarin on inflammatory injury in human umbilical vein endothelial cells (HUVECs). Manipulation of miRNA levels in HUVECs was achieved by transfecting cells with miR-570-3p mimic and inhibitor. Overexpression of miR-570-3p could decrease the expression of downstream components of HDAC1 including TNF-α, IL-1β, and malondialdehyde, while increasing GSH-Px activity in HUVECs under hyperglycemic conditions. Nevertheless, such phenomenon was completely reversed by miR-570-3p inhibitor, and administration of miR-570-3p inhibitor could block the inhibition of vaccarin on HDAC1 and inflammatory injury. Luciferase reporter assay confirmed the 3′- UTR of the HDAC1 gene was a direct target of miR-570-3p. In summary, our findings suggest that vaccarin alleviates endothelial inflammatory injury in diabetes by mediating miR-570-3p/HDAC1 pathway. Our study provides a new pathogenic link between deregulation of miRNA expression in the vascular endothelium of diabetes and inflammatory injury and provides new ideas, insights, and choices for the scope of application and medicinal value of vaccarin and some potential biomarkers or targets in diabetic endothelial dysfunction and vascular complications.

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Inhibition of miR-383 suppresses oxidative stress and improves endothelial function by increasing sirtuin 1

Cited by 10 publications

References 35 publications

The role of oxidative stress in diabetes mellitus-induced vascular endothelial dysfunction

The role of oxidative stress in diabetes mellitus-induced vascular endothelial dysfunction

Analysis of the Resistance of Small Peptides from Periplaneta americana to Hydrogen Peroxide-induced Apoptosis in KGN Cells based on High-throughput miRNA Sequencing

Vaccarin alleviates endothelial inflammatory injury in diabetes by mediating miR-570-3p/HDAC1 pathway

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