Effect of bisacodyl on rats with slow transit constipation

Abstract

The effect of bisacodyl on the treatment of rats with slow transit constipation (STC) was studied. Forty-five female Wister rats were divided into control group, STC group, and STC bisacodyl group. The immunohistochemical method was used to determine interstitial cells of Cajal (ICC) and the expression of c-Kit protein. Body mass and the number of defecations were significantly decreased in the STC group compared with the control group on the 100th day after diphenoxylate administration, while dry weight of feces was significantly increased and the intestinal transit time was prolonged. There were significant differences in the number of defecations, dry weight of feces, and intestinal transit time among the three groups. The number of defecations was higher, dry weight of feces was lower, and intestinal transit time was shorter in the STC bisacodyl group compared to the STC group. In addition, ICC basement membrane dissolution occurred in the colon wall of the STC group. The connection between ICC and surrounding cells was destroyed, and the nucleus shrunken to different degrees. Moreover, c-Kit expression in the STC group was significantly lower than the control group. The connection between ICC and surrounding cells in the STC bisacodyl group was significantly stronger than the STC group, and the number of ICC and the expression of c-Kit were increased. Bisacodyl could reduce the severity of STC in rats by increasing the number of ICC and the expression of c-Kit.

Exploring Molecular Mechanisms of Aloe barbadmsis Miller on Diphenoxylate-Induced Constipation in Mice

“…As was known that diphenoxylate often used as a modeling agent in constipation mice or rat model for its side effects that it could lead to constipation by inhibiting peristalsis, delaying the passage of intestinal contents, and increasing the absorption of water in the intestine [37]. In our experiment, constipation mice induced by diphenoxylate was used according to previous studies [38,39]. Aloe was usually taken orally to resolve heat and diminish inflammation to treat constipation [40].…”

METTL3 contributes to slow transit constipation by regulating miR‐30b‐5p/PIK3R2/Akt/mTOR signaling cascade through DGCR8

“…2,12 ICCs are pacemaker cells that play pivotal roles in gastrointestinal myoelectric activity and gastrointestinal motility, which were distributed between the nerve endings and smooth muscle cells in the gastrointestinal tract. 13,14 As a group, as we know, the gradually increasing incidence of STC is closely associated with the morphological abnormality and quantity reduction of ICCs. Autophagy is a conserved intracellular process for the degradation of dysfunctional organelles, misfolded proteins, and oxidatively damaged proteins by lysosomes in eukaryotic cells.…”

“…Emerging evidences have delineated the decreased number of interstitial cells of Cajal (ICCs) was closely related to the pathophysiology of STC 2,12 . ICCs are pacemaker cells that play pivotal roles in gastrointestinal myoelectric activity and gastrointestinal motility, which were distributed between the nerve endings and smooth muscle cells in the gastrointestinal tract 13,14 . As a group, as we know, the gradually increasing incidence of STC is closely associated with the morphological abnormality and quantity reduction of ICCs.…”

Establishment of high‐performance liquid chromatography‐photodiode array method for 43 weight loss agents and effect of basic environment on bisacodyl degradation

“…Bisacodyl (IUPAC name: [4‐[(4‐acetyloxyphenyl)‐pyridin‐2‐ylmethyl]phenyl] acetate), a diphenylmethane derivative, is an organic compound used as a stimulant laxative for short‐term treatment of constipation by directly stimulating the rectum and colon 9,10 . Recently, it has also been used to prepare the intestines before surgery or endoscopy, including colonoscopy and sigmoidoscopy 11 .…”