Long non-coding RNA LINC00261 sensitizes human colon cancer cells to cisplatin therapy

Abstract

Colon cancer is one of the most common digestive tumors. The present study aimed to explore the functional role, as well as the underlying mechanism of long non-coding RNA LINC00261 in colon cancer. Expression of LINC00261 was analyzed in colon cancer cell lines and human normal cell lines. Acquired resistance cell lines were then built and the acquired resistance efficiency was detected by evaluating cell viability. Thereafter, the effects of LINC00261 overexpression on cisplatin-resistant colon cancer cells were measured, as well as cell apoptosis, viability, migration, and invasion. Subsequently, we investigated the interaction of LINC00261 and β-catenin. The results showed that the LINC00261 gene was down-regulated in colon cancer cell lines and tissues, and in cisplatin-resistant cells. LINC00261 overexpression might relieve cisplatin resistance of colon cancer cells via promoting cell apoptosis, and inhibiting cell viability, migration, and invasion. Moreover, LINC00261 might down-regulate nuclear β-catenin through restraining β-catenin from cytoplasm into nuclei or it could also promote β-catenin degradation and inhibit activation of Wnt pathway. Finally, LINC00261 reduced cisplatin resistance of colon cancer in vivo and enhanced the anti-colon cancer effect of cisplatin through reducing tumor volume and weight.

Long noncoding RNA LINC00261 regulates endometrial carcinoma progression by modulating miRNA/FOXO1 expression

“…Although our data showed similar LINC00261 functions on cancer cell aggressiveness as reported previously, 15,[17][18][19]23 the molecular mechanisms are totally different. In colon cancer, LINC00261 downregulates Wnt signalling activity through restraining β-catenin from cytoplasm into nuclei and promoting β-catenin degradation.…”

“…Now, we first time reported that LINC00261 was also downregulated in endometrial carcinoma, and the LINC00261 levels were further decreased in endometrial carcinoma with lymphatic metastasis. Although our data showed similar LINC00261 functions on cancer cell aggressiveness as reported previously, the molecular mechanisms are totally different. In colon cancer, LINC00261 downregulates Wnt signalling activity through restraining β‐catenin from cytoplasm into nuclei and promoting β‐catenin degradation .…”

“…Although our data showed similar LINC00261 functions on cancer cell aggressiveness as reported previously, the molecular mechanisms are totally different. In colon cancer, LINC00261 downregulates Wnt signalling activity through restraining β‐catenin from cytoplasm into nuclei and promoting β‐catenin degradation . In gastric cancer, LINC00261 interacts and destabilizes Slug protein, which is involved in epithelial‐mesenchymal transitions and has antiapoptotic activity .…”

“…In this study, we found that lncRNA LINC00261 was downregulated in endometrial carcinoma and associated with metastasis of this cancer. LINC00261 has been reported to be downregulated in multiple cancers, including colon cancer, 23 stomach cancer, 24 gastric cancer, 15,25 nonsmall cell lung cancer, 18 and hepatocellular cancer. 19 Now, we first time reported that LINC00261 was also downregulated in endometrial carcinoma, and the LINC00261 levels were further decreased in endometrial carcinoma with lymphatic metastasis.…”

“…In colon cancer, LINC00261 downregulates Wnt signalling activity through restraining β-catenin from cytoplasm into nuclei and promoting β-catenin degradation. 23 In gastric cancer, LINC00261 interacts and destabilizes Slug protein, which is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. 26 FIGURE 3 LINC00261 is a molecular sponge for miR-182, miR-183, miR-153, miR-27a, and miR-96.…”