Investigation of modified platelet-rich plasma (mPRP) in promoting the proliferation and differentiation of dental pulp stem cells from deciduous teeth

Wen, Jianbo; Li, H.T.; Li, S.H.; Li, Xiongjie; Duan, Jianmin

doi:10.1590/1414-431x20165373

Cited by 20 publications

(18 citation statements)

References 23 publications

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“…This is in agreement with other studies, which reported that higher concentrations (10%, 20%) of PRP induced significantly less proliferation compared to lower concentrations (2%, 5%) [59,60] possibly due to the inhibitory effect of prostaglandin E2 released by the platelet concentrate [61]. Two percent PRP seems to be the ideal concentration for the growth of exfoliated deciduous tooth MSCs [60]. The most surprising results in the current study were those of the 3-day cell proliferation assay.…”

Section:  Discussionsupporting

confidence: 93%

Injectable platelet-rich fibrin influences the behavior of gingival mesenchymal stem cells

Iozon

Caracostea

Páll³

et al. 2020

Rom J Morphol Embryol

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In this study, we examined the effects of injectable platelet-rich fibrin (iPRF) on proliferation and osteodifferentiation in mesenchymal stem cells (MSCs) isolated from human gingiva. Gingival MSCs (gMSCs) were grown in experimental culture media with different concentrations of iPRF [5%, 10%, and replacement of fetal calf serum (FCS) in the standard media with 10% iPRF-10% iPRF-FCS]. Immunophenotyping of gMSCs was performed after seven days by flow cytometry, and their proliferation was examined after three and seven days using the Cell Counting Kit-8 method. After 14 days in culture, spontaneous osteogenic differentiation of gMSCs was evaluated via real-time polymerase chain reaction. All gMSCs were positive for cluster of differentiation (CD) 105, CD73, CD90, and CD44, and negative for CD34/45, CD14, CD79a, and human leukocyte antigen, DR isotype (HLA-DR). Reduced expression of some surface antigens was observed in the gMSCs grown in 10% iPRF-FCS medium compared to the other groups. After three days, gMSCs grown in 10% iPRF had proliferated significantly less than the other groups. After seven days, proliferation was significantly higher in the 5% iPRF cells compared to the control, while proliferation in the 10% iPRF and 10% iPRF-FCS groups was significantly lower. No spontaneous osteogenic differentiation was observed in the presence of iPRF, as observed by low runt-related transcription factor 2 (RUNX2) expression. Some expression of secreted protein acidic and cysteine rich (SPARC) and collagen 1 alpha (COL1A) was observed for all the gMSCs regardless of the culture medium composition. gMSCs grown in 10% iPRF had significantly lower SPARC expression. In conclusion, 5% iPRF stimulated gMSC proliferation, and an excessively high concentration of iPRF can impair osteogenic induction.

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Section:  Discussionsupporting

confidence: 93%

Injectable platelet-rich fibrin influences the behavior of gingival mesenchymal stem cells

Iozon

Caracostea

Páll³

et al. 2020

Rom J Morphol Embryol

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“…The cells outgrowing from the explants and the differentiation of DP-MSCs to three lineages in vitro are depicted in Figure 1 . In agreement with a recent report [ 9 ], we found extract of human platelets to be an optimal growth supplement for the expansion of DP-MSCs for clinical scale manufacturing. Additionally, DP-MSCs do not differ substantively from MSCs derived from adipose tissue, bone marrow, and umbilical cord tissue with regard to the internationally accepted vague criteria of plastic adherence and the ability to differentiate into osteoblasts, chondrocytes, and adipocytes in vitro while expressing mesenchymal (CD29, CD90, CD105, CD73, and CD44) but not hematopoietic lineage markers (CD14, CD34, and CD45) [ 10 ].…”

Section: Dental Pulp Mesenchymal Stem/stromal Cellssupporting

confidence: 93%

Dental Mesenchymal Stem/Stromal Cells and Their Exosomes

Stanko

Altanerova²,

Jakubechova³

et al. 2018

Stem Cells International

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Stem cells derived from human dental pulp tissue (DP-MSC) differ from the other mesenchymal stem cells prepared from bone marrow or adipose tissue due to their embryonic origin from the neural crest and are of special interest because of their neurotropic character. Furthermore, the therapeutic potential of DP-MSCs is realized through paracrine action of extracellularly released components, for which exosomes play an important role. In this review, we intend to explore the properties of these cells with an emphasis on exosomes. The therapeutic applicability of these cells and exosomes in dental practice, neurodegenerative diseases, and many other difficultly treatable diseases, like myocardial infarction, focal cerebral ischemia, acute lung or brain injury, acute respiratory distress syndrome, acute inflammation, and several others is concisely covered. The use of cellular exosomes as an important diagnostic marker and indicator of targeted cancer therapies is also discussed, while the importance of stem cells from human exfoliated deciduous teeth as a source of evolutionally young cells for future regenerative therapies is stressed. We conclude that exosomes derived from these cells are potent therapeutic tools for regenerative medicine in the near future as clinical administration of DP-MSC-conditioned medium and/or exosomes is safer and more practical than stem cells.

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“…A few studies pointed out that PRP could improve odontogenic/osteogenic differentiation [13,34,35] and mineralization [11,12] of dental stem cells. PRP promoted mRNA and protein expression of DSPP and DMP-1 in SCAP [13].…”

Section: Discussionmentioning

confidence: 99%

Effects of Platelet-Rich Plasma on Proliferation, Viability, and Odontogenic Differentiation of Neural Crest Stem-Like Cells Derived from Human Dental Apical Papilla

Xiang

Guan

et al. 2020

BioMed Research International

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Objective. This study is aimed at evaluating the effects of platelet-rich plasma (PRP) on proliferation, viability, and odontogenic differentiation of neural crest stem-like cells (NCSCs) derived from human dental apical papilla. Materials and Methods. Cells from apical papillae were obtained and then induced to form neural spheres. The expression of NCSC markers p75NTR and HNK-1 in neural sphere cells was detected by immunofluorescence staining. Human PRP was prepared by a 2-step centrifugation method and activated by CaCl2 and thrombin. The concentrations of PDGF-BB and TGF-β1 in whole blood and PRP were measured by an ELISA kit. PRP in five different concentrations (0%, 2.5%, 5%, 10%, and 25%) was applied to culture NCSCs. On the 1st, 3rd, 5th, and 7th days, cell proliferation was evaluated by CCK8. Cell viability was tested by a live/dead staining kit. mRNA and protein expression of DSPP and BMP4 were analyzed by RT-qPCR and western blot, respectively. Statistical analysis was performed by a one-way analysis of variance (ANOVA) test or t-test. Results. Dental apical papilla cells formed neural spheres, from which cells displayed positive expression of p75NTR and HNK-1. The concentrations of PDGF-BB and TGF-β1 in PRP were about 3.5-fold higher than those in whole blood. 5% and 10% PRP significantly promoted proliferation of NCSCs, while 25% and 50% PRP inhibited cell proliferation from Day 3 to Day 7. Low-concentration (2.5%, 5%, and 10%) PRP slightly improved viability of NCSCs on Day 7. On the other hand, high-concentration (25% and 50%) PRP significantly inhibited viability of NCSCs from Day 3 to Day 7. RT-qPCR and western blot results indicated that 10% PRP could promote odontogenic differentiation of NCSCs on Day 7. mRNA and protein expression of DSPP and BMP4 were significantly upregulated in the 10% PRP group compared to those in the control group (P<0.05). Conclusions. PRP is a simply acquirable blood derivative which contains high concentration of growth factors like PDGF-BB and TGF-β1. PRP in a proper concentration could promote proliferation, viability, and odontogenic differentiation of NCSCs derived from human dental apical papilla.

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Investigation of modified platelet-rich plasma (mPRP) in promoting the proliferation and differentiation of dental pulp stem cells from deciduous teeth

Cited by 20 publications

References 23 publications

Injectable platelet-rich fibrin influences the behavior of gingival mesenchymal stem cells

Injectable platelet-rich fibrin influences the behavior of gingival mesenchymal stem cells

Dental Mesenchymal Stem/Stromal Cells and Their Exosomes

Effects of Platelet-Rich Plasma on Proliferation, Viability, and Odontogenic Differentiation of Neural Crest Stem-Like Cells Derived from Human Dental Apical Papilla

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