Salt-induced epithelial-to-mesenchymal transition in Dahl salt-sensitive rats is dependent on elevated blood pressure

Wang, Y.; Mu, Jianjun; Liu, F.Q.; Ren, Keyu; Xiao, Hong; Zhou, Yang; Yuan, Zuyi

doi:10.1590/1414-431x20133554

Cited by 20 publications

(15 citation statements)

References 27 publications

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“…Finally, the adherens junction pathway was enriched in the KSD-NTN group compared to the KSD-HTN group. In a previous study in rats, expression of the adherens junction protein E-cadherin was shown to be negatively correlated with BP [55]. These results indicate that the adherens junction pathways of the urinary microbiome may be involved in the occurrence or development of KSD-HTN.…”

Section: Discussionmentioning

confidence: 55%

Characteristics of the urinary microbiome in kidney stone patients with hypertension

et al. 2020

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Background: Kidney stone disease (KSD) is more common in individuals with hypertension (HTN) than in individuals with normotension (NTN). Urinary dysbiosis is associated with urinary tract disease and systemic diseases. However, the role of the urinary microbiome in KSD complicated with HTN remains unclear. Methods: This study investigated the relationship between the pelvis urinary microbiome and blood pressure (BP) in patients with KSD co-occurring with HTN (KSD-HTN) and healthy controls (HC) by conducting 16S rRNA gene sequencing of bacteria in urine samples. The urine samples were collected (after bladder disinfection) from 50 patients with unilateral kidney calcium stones and NTN (n = 12), prehypertension (pHTN; n = 11), or HTN (n = 27), along with 12 HCs. Results: Principal coordinates analysis showed that there were significant differences in the urinary microbiomes not only between KSD patients and HCs but also between KSD-pHTN or KSD-HTN patients and KSD-NTN patients. Gardnerella dominated in HCs, Staphylococcus dominated in KSD-NTN patients and Sphingomonas dominated in both KSD-pHTN and KSD-HTN patients. The abundance of several genera including Acidovorax, Gardnerella and Lactobacillus was correlated with BP. Adherens junction and nitrogen and nucleotide metabolism pathways, among others, were associated with changes in BP. Conclusions: The findings suggest that patients with KSD complicated with HTN have a unique urinary microbiome profile and that changes in the microbiome may reflect disease progression and may be useful to monitor response to treatments.

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Section: Discussionmentioning

confidence: 55%

Characteristics of the urinary microbiome in kidney stone patients with hypertension

et al. 2020

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“…Immunohistochemistry was conducted as previously reported [21]. Briefly, serial 5 µm paraffin-embedded tissue sections were dewaxed and rehydrated.…”

Section: Methodsmentioning

confidence: 99%

“…The primers employed in this study are shown in Table 1. The protocol for RT-PCR was based on our previous method [21]. The mRNA expression values are shown in folds of mRNA expression in the tissues of SD rats fed with normal salt diet.…”

Section: Methodsmentioning

confidence: 99%

Effects of Renin-Angiotensin System Inhibitors on Renal Expression of Renalase in Sprague-Dawley Rats Fed With High Salt Diet

Wang

Xie

Gao³

et al. 2015

Kidney Blood Press Res

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Background/Aims: The aim of our study was to investigate the effect of high-salt diet on the renal expression of renalase and the potential role of the local renin-angiotensin system in this process. Methods: Sprague-Dawley (SD) rats were divided into groups according to salt content in diet and drug treatment as follows: normal-salt diet (NS), high-salt diet (HS), high-salt intake with hydralazine (HS+H), high-salt diet with enalapril (HS+E), and high-salt diet with valsartan (HS+V). The dietary intervention and drugs were given for four weeks. Renin activity and angiotensin II type 1 receptor (AT1R) levels were detected by real-time PCR. Renalase mRNA and protein were also measured. Results: After four weeks, systolic blood pressure and proteinuria were significantly increased in the HS group with respect to the NS group. Dietary salt intake caused a dramatic decrease in renalase expression in the rat kidneys. Renal cortex renin and AT1R increased significantly in the HS and HS+H groups. Urinary protein was positively correlated with renal renin and AT1R levels. However, in the HS+E and HS+V groups, enalapril and valsartan failed to influence renal renalase expression but abolished the increase in proteinuria, renal cortex renin, and AT1R levels with respect to the HS group. Conclusion: This study indicates that high salt intake reduces renal expression, and renal RAS may be not involved in the regulation of renalase in SD rats fed with high-salt diet.

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“…Salt overload greatly contributes to the development and progression of hypertension [ 1 , 2 ]. Kidney and the vasculature are two critical parts of the body that regulate sodium balance, which ultimately develop into aberrant structures and dysfunction in high-salt diets [ 3 – 5 ]. Despite the detrimental effects of salt on the genesis of hypertension and sodium-regulated organs, humans and animals have various compensatory pathways to maintain sodium balance under high-salt conditions.…”

Section: Introductionmentioning

confidence: 99%

Effect of Salt Intervention on Serum Levels of Fibroblast Growth Factor 23 (FGF23) in Chinese Adults: An Intervention Study

Wang

Chu

et al. 2018

Med Sci Monit

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BackgroundFibroblast growth factor 23 (FGF23), a prominent regulator of phosphate and calcium metabolism, regulates sodium excretion in distal tubules through sodium-chloride cotransporter. This effect regulates blood pressure. Salt intake exerts effects on serum levels of FGF23 in mice. The aim of this study was to explore whether salt intervention affects serum concentrations of FGF23 in Chinese adults.Material/MethodsWe enrolled 44 participants from Lantian, a rural community of Shaanxi, China. All participants were maintained on a three-day normal diet, which was sequentially followed by a seven-day low-Na+ diet and seven-day high-Na+ diet. Serum FGF23 concentrations were assessed by ELISA.ResultsSerum FGF23 concentrations elevated during low-salt diet compared with levels at baseline (66.20±44.21 pg/mL versus 86.77±53.74 pg/mL, p<0.05) and remarkably decreased when changed from low to high salt intake (86.77±53.74 pg/mL versus 49.26±42.67 pg/mL, p<0.001). Responses of FGF23 to salt intervention were more prominent in normotensive, older than 60 years, BMI <24 kg/m2 and salt-resistant individuals. Furthermore, a significant inverse correlation was observed between 24-hour urinary sodium and serum concentrations of FGF23 after adjusting age, sex, BMI and hypertension status.ConclusionsDietary salt intervention significantly affects serum FGF23 in Chinese adults.

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Salt-induced epithelial-to-mesenchymal transition in Dahl salt-sensitive rats is dependent on elevated blood pressure

Cited by 20 publications

References 27 publications

Characteristics of the urinary microbiome in kidney stone patients with hypertension

Characteristics of the urinary microbiome in kidney stone patients with hypertension

Effects of Renin-Angiotensin System Inhibitors on Renal Expression of Renalase in Sprague-Dawley Rats Fed With High Salt Diet

Effect of Salt Intervention on Serum Levels of Fibroblast Growth Factor 23 (FGF23) in Chinese Adults: An Intervention Study

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