VP22 herpes simplex virus protein can transduce proteins into stem cells

Gabanyi, Ilana; Lojudice, Fernando Henrique; Kossugue, Patrícia M.; Rebelato, Eduardo; Demasi, Marcos Angelo Almeida; Sogayar, Mari Cleide

doi:10.1590/1414-431x20122148

Cited by 4 publications

(2 citation statements)

References 19 publications

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“…Ciprofloxacin (CIP) has been used as an antibiotic prophylaxis for the prevention of bacterial infection in patients receiving stem cell transplant and in stem cell research [ 19 , 20 ]. Also, this considerably safe drug is widely used in the treatment of certain infection in cell culture [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

Ciprofloxacin Improves the Stemness of Human Dermal Papilla Cells

Kiratipaiboon

Tengamnuay

Chanvorachote

2016

Stem Cells International

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Improvement in the expansion method of adult stem cells may augment their use in regenerative therapy. Using human dermal papilla cell line as well as primary dermal papilla cells as model systems, the present study demonstrated that ciprofloxacin treatment could prevent the loss of stemness during culture. Clonogenicity and stem cell markers of dermal papilla cells were shown to gradually decrease in the culture in a time-dependent manner. Treatment of the cells with nontoxic concentrations of ciprofloxacin could maintain both stem cell morphology and clonogenicity, as well as all stem cells markers. We found that ciprofloxacin exerted its effect through ATP-dependent tyrosine kinase/glycogen synthase kinase3β dependent mechanism which in turn upregulated β-catenin. Besides, ciprofloxacin was shown to induce epithelial-mesenchymal transition in DPCs as the transcription factors ZEB1 and Snail were significantly increased. Furthermore, the self-renewal proteins of Wnt/β-catenin pathway, namely, Nanog and Oct-4 were significantly upregulated in the ciprofloxacin-treated cells. The effects of ciprofloxacin in preserving stem cell features were confirmed in the primary dermal papilla cells directly obtained from human hair follicles. Together, these results revealed a novel application of ciprofloxacin for stem cell maintenance and provided the underlying mechanisms that are responsible for the stemness in dermal papilla cells.

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Section: Introductionmentioning

confidence: 99%

Ciprofloxacin Improves the Stemness of Human Dermal Papilla Cells

Kiratipaiboon

Tengamnuay

Chanvorachote

2016

Stem Cells International

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“…On the other hand, this drug has moderate effects on gram-positive bacteria including Streptococcus pneumonia and Streptococcus faecalis. The ciprofloxacin is mainly indicated in the treatment of respiratory infections (other than Streptococcus pneumonia infection), urinary tract infections, gastrointestinal infections, including typhoid fever, bursitis, and septicemia, which are susceptible to microorganisms (21,22). Furthermore, Ciprofloxacin is used as a prophylactic antibiotic to prevent bacterial infections in patients undergoing stem cell transplantation.…”

Section: Discussionmentioning

confidence: 99%

Increased Expression of Oct-4 and Nanog Genes Following Nontoxic Ciprofloxacin Treatment in Adipose-Derived Stem Cells

Tabaie

Moghadam

Zarrabi

et al. 2019

Middle East J Rehabil Health Stud

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Background: Recently, because of the demand for cell therapy, stem cells researches have been increased. Improvement in stem cell proliferation promotes the use of these cells in cellular treatments. Objectives: The aim of this study was to improve the culture conditions of adipose-derived stem cells (ADSCs) by treatment of these cells with a nontoxic concentration of ciprofloxacin. Methods: ADSCs were prepared from the human and animal cell bank of Iranian Biological Resource Center. The microbial contamination of cells including bacteria, fungi, and yeast had been tested. Adipogenic and osteogenic differentiation potential of ADSCs was demonstrated and the expression of surface markers including CD45, CD29, CD90, CD34, and CD105 was confirmed by flow cytometry. In the presence of ciprofloxacin at different concentrations, the viability of the cells was measured by MTT assay and the expression of the Oct-4, Nanog and GAPDH were examined by real-time PCR in a nontoxic concentration of ciprofloxacin-treated cells compared to control group. Results: The results showed that 1.5, 3, 6, and 12 mg/mL concentrations of ciprofloxacin have toxic effects on ADSCs (P < 0.0001) while in 0.5-150 µg/mL concentrations, there are no toxic effects on the cells. Furthermore, Oct-4 and Nanog expression in the nontoxic concentration of ciprofloxacin-treated cells was increased compared to the control group, which indicates the positive effect of ciprofloxacin on the growth of these cells. Conclusions: The present study shows that due to increased expression of Oct-4 and Nanog in 10 µg/mL ciprofloxacin-treated cells, the use of ciprofloxacin during the ADSCs culture period could provide suitable conditions for maintaining stem cell properties in these cells.

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Development of Cell Penetrating Peptides for Effective Delivery of Recombinant Factors into Target Cells

Vijakumaran

Nordin

Hamid

et al. 2020

PPL

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: The cell membrane is a protective layer that strictly controls the passage of molecules restricting the delivery of biomolecules such as drugs, oligonucleotides, peptides, and siRNA into the cells. This shortcoming has been overcome by the discovery of Cell-Penetrating Peptides (CPPs) that has undergone 30 years of evolution. To date, CPPs are largely modified to improve its efficacy and to suit the different delivery applications. The modes of CPPs penetration are still an unresolved mystery and requires further investigations to increase its effectiveness and to diversify its use. Despite having huge potential as a biomolecule carrier, CPPs also have some drawbacks. In this review, the natural and synthetic CPPs, the modifications that have been conducted on CPPs to improve its efficacy, its extended applications, modes of penetration and limitation as well as challenges will be discussed.

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VP22 herpes simplex virus protein can transduce proteins into stem cells

Cited by 4 publications

References 19 publications

Ciprofloxacin Improves the Stemness of Human Dermal Papilla Cells

Ciprofloxacin Improves the Stemness of Human Dermal Papilla Cells

Increased Expression of Oct-4 and Nanog Genes Following Nontoxic Ciprofloxacin Treatment in Adipose-Derived Stem Cells

Development of Cell Penetrating Peptides for Effective Delivery of Recombinant Factors into Target Cells

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