Ocorrência De Anticorpos Contra O Herpesvírus Equino E Virus Da Arterite Equina Em Rebanhos Equinos Do Estado Do Rio De Janeiro

Abstract

<title>Resumo</title><p>A infecção pelo herpesvírus equino (EHV) e o vírus da arterite equina (EAV) tem sido associada a perdas econômicas importantes para a equideocultura em nível mundial. Estudos sorológicos têm demonstrado a circulação destes agentes no Brasil. O presente trabalho teve como objetivo principal estudar a ocorrência de anticorpos específicos contra o EAV e EHV em cavalos oriundos de diferentes regiões do estado do Rio de Janeiro. Para este propósito, foram coletadas amostras de soro de 581 animais não vacinados e de 44 éguas provenientes de um plantel vacinado regularmente contra a rinopneunonite equina. Todas as amostras foram submetidas ao teste de soroneutralização para a pesquisa de anticorpos específicos contra cada um dos vírus. Os resultados demonstraram 29,6% (172/581) de animais soropositivos para o EHV (títulos entre 2 e ≥ 256) e 0,79% (05/630) para o EAV (títulos entre 2 e 4.096). Considerando a sorologia positiva em animais não imunizados, os achados demonstram a indução de anticorpos específicos após exposição natural aos respectivos vírus, indicando uma possível circulação desses agentes nos rebanhos analisados.</p>

Inhibition of equine arteritis virus by an antimicrobial peptide produced by Bacillus sp. P34

“…Cytotoxicity assays are necessary to define the concentration range for further and more detailed in vitro testing to provide meaningful information on parameters such as genotoxicity or programmed cell death (Eisenbrand et al, 2002). Considering that treatment with AMPs is potentially effective and non-toxic to humans and animals, it has already been proposed as an alternative for disease control (Galdiero et al, 2013).…”

“…Meanwhile, reports of a respiratory disease similar to equine viral arteritis (EVA), which presented abortion and was transmitted venereally occurred still at the end of the XIX century (Pottie, 1888), suggesting that EAV has been present as an infectious agent inside the equine population for a long time (Glaser et al, 1996). Although EAV has still not been isolated in Brazil, there is serological evidence of its presence in different regions of the country (Bello et al, 2007;Diaz et al, 2015;Sartori et al, 2016).…”

Identification of equine herpesvirus type 1 as cause of abortion in mares in Southern Brazil

“…Infection with this virus may cause a serious economic impact in the horse industry, especially for breeding farms (Gilkerson et al, 1999). In Brazil, serological studies have demonstrated the circulation of EHV-1 in horses (Lara et al, 2010;Diaz et al, 2015). Latency has been demonstrated in both lymphoid and neural tissues and allows the virus to circulate silently in the horse populations (Patel et al, 2005).…”

“…Equine herpesvirus-1 abortion is diagnosed by detailed examination of the aborted fetus using a combination of gross findings, histopathology, virus isolation, polymerase chain reaction (PCR), and immunostaining (Reed et al, 2004). Conventionally, these tests have been applied to selected target organs, particularly the liver, lung, thymus and spleen (Easton et al, 2009). The main purpose of this study was to describe and confirm EHV-1 as cause of abortion in southern Brazil.…”

“…The epidemiological data of each case were obtained from the necropsy submission forms of the Laboratório Regional de Diagnóstico of the Universidade Federal de Pelotas, RS, Brazil (LRD/UFPel). Sections of paraffin embedded tissues were subjected to hematoxylin and eosin staining and samples of the liver, spleen and lungs were processed for immunohistochemical (IHC) analysis using a polyclonal antibody specific to EHV-1 (PAB-ERV -VMRD) and analyzed using a commercial streptavidinalkaline phosphatase kit (LSAB+System-AP, Dako Cytomation, K0689 [Dako Cytomation, 6392 Via Real, Carpinteria, CA, USA]) according to a previous study (Easton et al, 2009). Positive (a previously confirmed EHV-1 case) and negative controls (liver, spleen and lungs from a healthy, EHV-1-free horse) were included.…”