VITRO OF Melanoxylon brauna SCHOTT. MORPHOGENESIS: RESPONSIVENESS OF EXPLANTS TO PERMANENT AND TEMPORARY IMMERSION GROWTH REGULATORS

Silva, Elisa Regina da; Simões, Ingridh Medeiros; Baptista, Julcinara Oliveira; Schmildt, Edílson Romais; Lopes, José Carlos; Gonçalves, Elzimar de Oliveira; Caldeira, Marcos Vinícius Winckler; Alexandre, Rodrigo Sobreira

doi:10.1590/01047760202026012709

Cited by 4 publications

(3 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The same result was obtained in Melanoxylon brauna Schott. where the use of between 2 and 4 mg L -1 of BAP provided a higher number of shoots (Silva et al 2020). According to Brum et al (2002), the multiplication of sprouts with the use of BAP may be related to the influence of the genetic charge from the mother plant, of the growth regulator used to help with cellular division and in the break of the dormant state of axillary shoots, until then inhibited by apical dormancy.…”

Section: Discussionmentioning

confidence: 99%

Vegetative rescue and in vitro propagation of Persea willdenovii

et al. 2022

View full text Add to dashboard Cite

Persea willdenovii is a native species popularly known for its high potential for medicinal use. Due to problems in low seed production and germination, vegetative propagation appears promising. Thus, we seek with this work to evaluate techniques of vegetative rescue and the potential use of shoots obtained in tissue culture. Adult trees of the species were used to apply total and partial girdling treatments and obtain pruned branches as vegetative rescue techniques. Shoot production was monitored over time (90 to 240 days). Sprouts were used for in vitro propagation, and disinfestation treatments were carried out using biocides such as NaClO and Plant Preservative Mixture TM (PPM TM ), changing the time and concentrations of the products. Multiplication was also tested using doses of 6-benzylaminopurine (BAP) and media and the potential for callogenesis under combinations of BAP and Naphthalene Acetic Acid (ANA). The vegetative rescue by complete or semi-girdling has the potential to produce shoots, though pruned branches proved to be one of the best techniques for rescue. In the micropropagation, the use of biocides such NaOCl for a long period of time (2 % for 20 min) with PPM TM added to the culture medium shows a potential for disinfestation in the in vitro establishment. For in vitro multiplication, the usage of WPM and BAP (2 a 4 g L -1 ) promotes higher shoot length, shoot number and leaf number. For indirect organogenesis by leaf segments, the cultivation with BAP and ANA was not responsive to the induction of callogenesis.

show abstract

Section: Discussionmentioning

confidence: 99%

Vegetative rescue and in vitro propagation of Persea willdenovii

et al. 2022

View full text Add to dashboard Cite

show abstract

“…In the same study, as a function of the concentration of IBA applied, the rooting percentage ranged from 0.5% to 88% among the species. This demonstrates that other factors besides the exogenous application of auxin are involved in the formation of adventitious roots by plants, like the ontogenetic age (Wendling et al, 2014a, b;Silva et al, 2020).…”

Section: Vegetative Propagation By Cutting Techniquementioning

confidence: 97%

Rescue and vegetative propagation of Eremanthus erythropappus (DC.) MacLeish in natural stand

et al. 2021

View full text Add to dashboard Cite

The native stands of ‘candeia’ (Eremanthus erythropappus) have been explored through management plans due to the economic potential of essential oil. The rescue of adult trees, as well as the application of silvicultural techniques that favor the restoration of the stand, can contribute to the genetic conservation of this species. This study’s objective was to assess the efficiency of propagation techniques for the rescue of 26 matrices of ‘candeia’ in a natural managed stand and discussion about the rhizogenesis. In August 2017, trees were induced to regrowth by coppice, followed by exposure and scarification of roots. The emergence of shoots and morphology were evaluated according to the origin (i.e., stump or root). After that period, 19 matrices had their sprouts collected for the preparation of apical cuttings. Indole-3-butyric acid (IBA) was applied at the base of the cuttings. Cutting survival at greenhouse exit (GE), rooting at shade house exit (SHE), morphology and root anatomy were evaluated. In 189 days, the scarification of roots promoted 76.92% of budding. The percentage of sprouted matrices, number of shoots per matrice, length, diameter, and shoot length/diameter ratio increased over time. Only 12.2% of the cuttings survived in GE, and of these, 7.9% rooted in SHE. The cutting resulted in the formation of a clonal mini-garden of ‘candeia’, with seven of the 19 matrices submitted to propagation. The anatomical analyses showed that bud formation occurs from cell redifferentiation in the phloem parenchyma, and presence of crystals on the walls of the vessel elements of the secondary xylem. The shoots induction from scarification of roots could be used as a silvicultural practice for the reestablishment of the native fragments handle.

show abstract

“…The axillary nodes showed active centers, whose shoot growth potential was similar to the potential of the apical meristem. However, activity in these centers resumes from the region closest to the cotyledonary node (Long & Barton, 2000;Silva et al, 2020). The basipetal movement of auxin produced in the apical meristem and transported to the roots results in different hormone levels throughout the plant.…”

mentioning

confidence: 99%

In vitro organogenesis as an efficient method for the propagation of Dalbergia nigra

Simões

Araújo

Mello

et al. 2022

Pesq. agropec. bras.

Self Cite

View full text Add to dashboard Cite

The objective of this work was to establish an efficient protocol for the in vitro organogenesis of Dalbergia nigra. For this purpose, 30-day-old seedlings were sectioned at their cotyledonary nodes and nodal segments. These materials were cultivated in a medium with different combinations of the 6-benzylaminopurine and thidiazuron cytokinins. After 60 days in a growth chamber set at 27°C and a 16-hour photoperiod, growth characteristics were analyzed. Explants from the cotyledonary nodes show a greater morphogenetic potential, regardless of the addition of cytokinins. Cotyledonary nodes in the medium supplemented with 2.22 µmol L-1 BAP show the best combination for the in vitro propagation of D. nigra.

show abstract

VITRO OF Melanoxylon brauna SCHOTT. MORPHOGENESIS: RESPONSIVENESS OF EXPLANTS TO PERMANENT AND TEMPORARY IMMERSION GROWTH REGULATORS

Cited by 4 publications

References 40 publications

Vegetative rescue and in vitro propagation of Persea willdenovii

Vegetative rescue and in vitro propagation of Persea willdenovii

Rescue and vegetative propagation of Eremanthus erythropappus (DC.) MacLeish in natural stand

In vitro organogenesis as an efficient method for the propagation of Dalbergia nigra

Contact Info

Product

Resources

About