In Vitro Germination of Melanoxylon Brauna Schott. And Evaluation of the Toxicity of Disinfecting Agents in the Lactuca Sativa L. Model Plant

Silva, Elisa Regina da; Simões, Ingridh Medeiros; Baptista, Julcinara Oliveira; Bighi, Kelly Nery; Fontes, Milene Miranda Praça; Schmildt, Edílson Romais; Lopes, José Carlos; Caldeira, Marcos Vinícius Winckler; Alexandre, Rodrigo Sobreira

doi:10.1590/01047760201925042688

Cited by 7 publications

(7 citation statements)

References 43 publications

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“…The highest percentage of normal seedlings (41.95%) was obtained with 14 min of exposure to NaOCl (Figure 1). NaOCl reduced contamination and, consequently, favored a higher percentage of normal seedlings of Melanoxylon brauna up to a maximum exposure time of 25 min (Silva et al, 2019). In D. nigra, above 14 min of exposure of the seeds to NaOCl, there was a reduction in seedling normality (Figure 1).…”

Section: Resultsmentioning

confidence: 95%

DISINFECTION PROTOCOL AND IN VITRO GERMINATION OF SEEDS OF Dalbergia nigra

Santos

Cezario

Simões

et al. 2020

CERNE

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Section: Resultsmentioning

confidence: 95%

DISINFECTION PROTOCOL AND IN VITRO GERMINATION OF SEEDS OF Dalbergia nigra

Santos

Cezario

Simões

et al. 2020

CERNE

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“…Soybean and maize seeds were rendered by the Agricultural Research and Extension Services (Gyeongsangbuk-do, South Korea) and Asia Seed Ltd. (Seoul, South Korea), respectively. Similar-sized seeds were selected and disinfected in 70% ethanol and 2.5% sodium hypochlorite and evaluated for vitality (Ke et al, 2018 ; Silva et al, 2020 ). Seeds were transferred onto pot trays (28 × 54 cm) filled with horticultural soil (Shinsung Mineral Co., Ltd., Chungcheongbuk-do, Korea), grown in a climate chamber (16/8 h day/night), and irrigated daily.…”

Section: Methodsmentioning

confidence: 99%

Salvianolic Acid Modulates Physiological Responses and Stress-Related Genes That Affect Osmotic Stress Tolerance in Glycine max and Zea mays

et al. 2022

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Drought is a serious threat worldwide to soybean and maize production. This study was conducted to discern the impact of salvianolic acid treatment on osmotic-stressed soybean (Glycine max L.) and maize (Zea mays L.) seedlings from the perspective of physiochemical and molecular reactions. Examination of varied salvianolic acid concentrations (0, 0.1, 1, 5, 10, and 25 μM) on soybean and maize seedling growth confirmed that the 0.1 and 1 μM concentrations, respectively, showed an improvement in agronomic traits. Likewise, the investigation ascertained how salvianolic acid application could retrieve osmotic-stressed plants. Soybean and maize seedlings were irrigated with water or 25% PEG for 8 days. The results indicated that salvianolic acid application promoted the survival of the 39-day-old osmotic-stressed soybean and maize plants. The salvianolic acid-treated plants retained high photosynthetic pigments, protein, amino acid, fatty acid, sugar, and antioxidant contents, and demonstrated low hydrogen peroxide and lipid contents under osmotic stress conditions. Gene transcription pattern certified that salvianolic acid application led to an increased expression of GmGOGAT, GmUBC2, ZmpsbA, ZmNAGK, ZmVPP1, and ZmSCE1d genes, and a diminished expression of GmMIPS2, GmSOG1, GmACS, GmCKX, ZmPIS, and ZmNAC48 genes. Together, our results indicate the utility of salvianolic acid to enhance the osmotic endurance of soybean and maize plants.

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“…Soybean seeds were provided by the Agricultural Research and Extension Services (Daegu, Gyeongsangbuk-do, Republic of Korea). Seeds of equal size/same color were picked and submerged in 70% ethanol and 2.5% sodium hypochlorite and checked for the effectiveness of the disinfection procedure and vitality [130,131]. Seeds (one seed/pot) were sown in pot trays (28 cm × 54 cm) filled with horticultural soil (Shinsung Mineral Co., Ltd., Daegu, Chungcheongbuk-do, Republic of Korea).…”

Section: Selection Of Appropriate Concentrations Of Salt Cadmium Lead and Nickelmentioning

confidence: 99%

“…The seeds were surface sterilized and tested for viability as stated by a formerly explained method [130,131]. Plants were grown in a climate chamber under the above mentioned conditions.…”

Section: Selection Of the Appropriate Ampelopsin (Amp) Concentrationmentioning

confidence: 99%

Ampelopsin Confers Endurance and Rehabilitation Mechanisms in Glycine max cv. Sowonkong under Multiple Abiotic Stresses

Kazerooni

Al-Sadi

Kim

et al. 2021

IJMS

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The present investigation aims to perceive the effect of exogenous ampelopsin treatment on salinity and heavy metal damaged soybean seedlings (Glycine max L.) in terms of physiochemical and molecular responses. Screening of numerous ampelopsin concentrations (0, 0.1, 1, 5, 10 and 25 μM) on soybean seedling growth indicated that the 1 μM concentration displayed an increase in agronomic traits. The study also determined how ampelopsin application could recover salinity and heavy metal damaged plants. Soybean seedlings were irrigated with water, 1.5% NaCl or 3 mM chosen heavy metals for 12 days. Our results showed that the application of ampelopsin raised survival of the 45-day old salinity and heavy metal stressed soybean plants. The ampelopsin treated plants sustained high chlorophyll, protein, amino acid, fatty acid, salicylic acid, sugar, antioxidant activities and proline contents, and displayed low hydrogen peroxide, lipid metabolism, and abscisic acid contents under unfavorable status. A gene expression survey revealed that ampelopsin application led to the improved expression of GmNAC109, GmFDL19, GmFAD3, GmAPX, GmWRKY12, GmWRKY142, and GmSAP16 genes, and reduced the expression of the GmERF75 gene. This study suggests irrigation with ampelopsin can alleviate plant damage and improve plant yield under stress conditions, especially those including salinity and heavy metals.

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In Vitro Germination of Melanoxylon Brauna Schott. And Evaluation of the Toxicity of Disinfecting Agents in the Lactuca Sativa L. Model Plant

Abstract: Sodium hypochlorite (NaOCl) alone is not efficient for disinfesting brauna seeds. Brauna seeds also require the use of captan fungicide (2%) 10 min-1 in disinfestation and maintenance of its residue.

Cited by 7 publications

References 43 publications

DISINFECTION PROTOCOL AND IN VITRO GERMINATION OF SEEDS OF Dalbergia nigra

DISINFECTION PROTOCOL AND IN VITRO GERMINATION OF SEEDS OF Dalbergia nigra

Salvianolic Acid Modulates Physiological Responses and Stress-Related Genes That Affect Osmotic Stress Tolerance in Glycine max and Zea mays

Ampelopsin Confers Endurance and Rehabilitation Mechanisms in Glycine max cv. Sowonkong under Multiple Abiotic Stresses

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