Pretreatments and enzymatic hydrolysis of sugarcane bagasse aiming at the enhancement of the yield of glucose and xylose

Guilherme, A. De A.; Dantas, Paulo Victor Fortunato; Soares, Jimmy; Santos, Everaldo Silvino dos; Fernandes, Fabiano A. N.; Macêdo, Gorete Ribeiro de

doi:10.1590/0104-6632.20170344s20160225

Cited by 36 publications

(11 citation statements)

References 44 publications

(64 reference statements)

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“…However, prolonging the freezing time to 3 h at the same temperature was followed by slightly insigni cant increment in the lignin and hemicellulose contents to 15.11% and 15.15 %, respectively. Similar trends were observed by Han-Seob Jeong (34), where the major contents of cellulose and hemicellulose and their derivatives in the liquid hydrolysates were affected until a freezing time of 2 h, after which, they remained relatively constant. Likely, the expansion of water volume inside the biomass during freezing fractionation was not critically affected by time.…”

Section: Resultssupporting

confidence: 84%

Synergetic Physical Freezing and Chemical Pretreatment of Lignocellulosic Sugarcane Bagasse Followed by Enzymatic Hydrolysis

Farghaly

Ahmed²,

Gad

2021

Preprint

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This study focuses on employing an eco-friendly pretreatment approach for lignocellulosic Sugarcane Bagasse (SCB) as a major problematic solid waste in Egypt, complying with the standard legislation as well. The applied technique depended on SCB physical fractionation via freezing, followed by chemical hydrolysis using alkaline hydrogen peroxide (AHP) and enzymatic hydrolysis. The changes occurred in macrostructure and the entire lignocellulosic compounds during the pretreatment stages were evaluated. Freezing fractionation resulted in relatively low glucose yield and saccharification ratio at -20°C for 2 h of 307.52 mg/gm native SCB and 48.5%, respectively, where no total reducing sugars (TRS) was obtained. Further AHP pretreatment was performed for the frozen-fractionated SCB at -20°C and 2 h with assistance of Box–Behnken Design response surface methodology (RSM). The investigated key parameters were H2O2 concentration (3, 5.5 and 8 %v/v), temperature (25, 42.5 and 60°C) and pretreatment duration (1, 3 and 5 h). The results revealed that the statistical modelling was able to predict the response of glucose yield and TRS production with R2 = 0.8221 and 0.8814, respectively. Applying the optimization tool of RSM, the optimum predicted values of glucose yield and TRS production were (886.51 mg/gm native SCB and 1.44 mg/mL), respectively; confirmed by the experimental analysis (898.5 mg/gm native SCB and 1.32 mg/mL), respectively. The coincided saccharification ratio was 97.5%. These results were obtained at H2O2 of 3 % (v/v), 56.93°C and 1 h which were 4.32 and 2.01 times higher than that obtained during the freezing pretreatment phase for glucose yield and saccharification ratio, respectively.

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Section: Resultssupporting

confidence: 84%

Synergetic Physical Freezing and Chemical Pretreatment of Lignocellulosic Sugarcane Bagasse Followed by Enzymatic Hydrolysis

Farghaly

Ahmed²,

Gad

2021

Preprint

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“…The enzymatic hydrolysis glucose yield was 53%, 65% and 85%, respectively, for enzyme load of 8, 12 and 16 FPU g −1 . Enzymatic hydrolysis of peroxide pretreated sugarcane bagasse was reported as 60% after 50 h of hydrolysis 35 . A study applied 10 FPU g −1 to hydrogen peroxide pretreated bagasse and observed a glucose yield of 51.60% 28 .…”

Section: Resultsmentioning

confidence: 99%

Sieving process selects sugarcane bagasse with lower recalcitrance to xylan solubilization

Alves

Melati

Casagrande

et al. 2020

J of Chemical Tech & Biotech

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BACKGROUNDSugarcane bagasse is a heterogeneous and organized material that needs a pretreatment to access the polysaccharides (cellulose and xylan). The particle size of a non‐milled bagasse can influence the xylan and xylose solubilization, as well as the enzymatic hydrolysis of the pretreated material. Using bagasse selected on 16‐, 30‐, 40‐ and 50‐mesh sieves: xylan solubilization was performed with hydrogen peroxide; xylose was solubilized with diluted sulfuric acid; and enzymatic hydrolysis was conducted for glucose yield evaluation.RESULTSThe xylose solubilized by acid pretreatment resulted in a maximum of 6.59 g L−1 with no influence of particle size. The solubilized xylan showed linear correlation with a variation of 36.18% (16‐mesh bagasse) to 71.43% (<50‐mesh bagasse). Pretreated material submitted to enzymatic hydrolysis revealed that particle size influenced the pretreatment, increasing the glucose yield.CONCLUSIONSThe results showed that non‐milled sugarcane bagasse particle size selection positively influenced the solubilization of xylan, improving the enzymatic hydrolysis of the pretreated material. © 2020 Society of Chemical Industry (SCI)

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“…De Guilherme et al [ 9 ] discussed disadvantages of using H 2 O 2 as it resulted in lesser degree of delignification and production of considerable foam during the reaction that caused the loss of solid matter. Similar observations were recorded in this study for H 2 O 2 pretreatment.…”

Section: Discussionmentioning

confidence: 99%

“…Hydrogen peroxide (H 2 O 2 ) pretreatment of SB was performed by swamping 4% (w/v) aqueous suspension of SB in a 7.35% (v/v) hydrogen peroxide solution. The slurry was stirred using a magnetic stirrer at 100 rpm for 1 h at room temperature [ 9 ] and filtered with Whatman No. 1 filter paper.…”

Section: Methodsmentioning

confidence: 99%

“…Reports suggest that alkaline pretreatment of SB is better than acid (H 2 SO 4 ) pretreatment as NaOH can dissolve more lignin in SB than H 2 SO 4 [8]. Hydrogen peroxide [9] and ionic liquids (ILs) are other solvents that have also been employed for pretreatment of biomass. In comparison to other solvents, ILs show valuable properties including non-combustibility, lower toxicity and low vapor pressure [10,11].…”

Section: Introductionmentioning

confidence: 99%

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Combined pretreatment of sugarcane bagasse using alkali and ionic liquid to increase hemicellulose content and xylanase production

et al. 2020

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Background Lignin in sugarcane bagasse (SB) hinders its utilization by microorganism, therefore, pretreatment methods are employed to make fermentable components accessible to the microbes. Multivariate analysis of different chemical pretreatment methods can aid to select the most appropriate strategy to valorize a particular biomass. Results Amongst methods tested, the pretreatment by using sodium hydroxide in combination with methyltrioctylammonium chloride, an ionic liquid, (NaOH+IL) was the most significant for xylanase production by Bacillus aestuarii UE25. Investigation of optimal levels of five significant variables by adopting Box-Behnken design (BBD) predicted 20 IU mL− 1 of xylanase and experimentally, a titer of 17.77 IU mL− 1 was obtained which indicated the validity of the model. The production kinetics showed that volumetric productivity of xylanase was much higher after 24 h (833.33 IU L− 1 h− 1) than after 48 h (567.08 IU L− 1 h− 1). The extracted xylan from SB induced more xylanase in the fermentation medium than pretreated SB or commercially purified xylan. Nuclear Magnetic Resonance, Fourier transform infrared spectroscopy and scanning electron microscopy of SB indicated removal of lignin and changes in the structure of SB after NaOH+IL pretreatment and fermentation. Conclusion Combined pretreatment of SB with alkali and methyltrioctylammonium chloride appeared better than other chemical methods for bacterial xylanase production and for the extraction of xylan form SB.

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Pretreatments and enzymatic hydrolysis of sugarcane bagasse aiming at the enhancement of the yield of glucose and xylose

Cited by 36 publications

References 44 publications

Synergetic Physical Freezing and Chemical Pretreatment of Lignocellulosic Sugarcane Bagasse Followed by Enzymatic Hydrolysis

Synergetic Physical Freezing and Chemical Pretreatment of Lignocellulosic Sugarcane Bagasse Followed by Enzymatic Hydrolysis

Sieving process selects sugarcane bagasse with lower recalcitrance to xylan solubilization

Combined pretreatment of sugarcane bagasse using alkali and ionic liquid to increase hemicellulose content and xylanase production

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