2017
DOI: 10.1590/0104-6632.20170341s20140222
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Abstract: A colorimetric sulfo-phospho-vanillin (SPV) method was used to validate a high throughput method for total lipids analysis in fresh and lyophilized oleaginous microorganisms. This method uses a small amount of biological material, does not require a lot of sample manipulation, can be automated, is reproducible and easy to implement. A 96-well microplate SPV assay allows one to determine quickly total lipids in lyophilized cells of oleaginous yeast and microalgae. The new assay method possesses many advantages … Show more

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Cited by 42 publications
(25 citation statements)
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“…No lipids were detected by the colorimetric sulfo‐phospho‐vanillin method suggesting lipid level was below the detection limit of 35 μ g ml −1 (Anschau et al . ). The amount of proteins and carbohydrates remains constant throughout the dry surface biofilm cycles with 95–97% of proteins and 3–5% carbohydrates in both CL and OL‐ DSB.…”
Section: Resultsmentioning
confidence: 97%
“…No lipids were detected by the colorimetric sulfo‐phospho‐vanillin method suggesting lipid level was below the detection limit of 35 μ g ml −1 (Anschau et al . ). The amount of proteins and carbohydrates remains constant throughout the dry surface biofilm cycles with 95–97% of proteins and 3–5% carbohydrates in both CL and OL‐ DSB.…”
Section: Resultsmentioning
confidence: 97%
“…The microplate assay showed a strong correlation with the gravimetric method for the analysis of four green microalgae strains. adapted the SPV method to a 96-well microplate assay, eliminating the first step of organic solvent addition (chloroform), and verified the impact of unsaturated and saturated fatty acids as a reference on the validation parameters of the method [190]. No significant difference in lipid determination was found between this and the gravimetric method, suggesting that the SPV approach could be a useful analytical tool to quantify lipids in lyophilized yeast and microalgae cells.…”
Section: Colorimetric Detectionmentioning
confidence: 94%
“…Quantification is then performed by measuring absorbance at 530 nm. The SPV method has been recommended for routine analysis; however, the presence of either double bonds or free hydroxyl groups is required for a positive SPV reaction [190]. To this end, its accuracy depends primarily on the reference standard being used.…”
Section: Colorimetric Detectionmentioning
confidence: 99%
“…A reação foi incubada a 50 °C, 120 rpm por 24 horas. Alíquotas de 0,1 mL foram retiradas periodicamente e preparadas para análises qualitativas e quantitativas, através do método colorimétrico sulfofosfo-vanilina (ANSCHAU et al, 2017; (SAMAD et al, 1989;CARDENAS et al, 2001). Em adição, o uso de corantes como: Rodamina B e Victoria Blue tem sido bastante utilizado para o screening de inúmeros microrganismos (KO et al, 2005;HASAN et al, 2009;AKBARI et al, 2011).…”
Section: Hidrólise Dos óLeos Vegetais Nativosunclassified
“…A concentração de ácidos graxos liberados em mg/mL foi quantificada através do método colorimétrico sulfofosfo-vanilina (ANSCHAU et al, 2017;.…”
Section: Hidrólise Dos óLeos Vegetaisunclassified