Closely-related Xanthomonas citri subsp. citri isolates trigger distinct histological and transcriptional responses in Citrus limon

Orce, Ingrid Georgina; Debes, Mario Alberto; Sendín, Lorena Noelia; Luque, Ana Catalina; Arias, Marta E.; Vojnov, Adrián Alberto; Maraño, María Rosa; Castagnaro, Atilio Pedro; Filippone, María Paula

doi:10.1590/0103-9016-2015-0409

Cited by 2 publications

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“…One of the most powerful strategies to defeat a phytopathogen disease is to understand the mechanism used by the pathogen to break down the host defense barriers. X. citri genome was published many years ago [5], and since then a strong effort has been done to understand the molecular pathways used by this bacterium during the infection process [6][7][8][9][10]. The virulence of X. citri is dependent on the translocation of effector proteins into the host cell through the type III secretion system, allowing the bacteria to cause disease [11].…”

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confidence: 99%

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mCherry fusions enable the subcellular localization of periplasmic and cytoplasmic proteins in Xanthomonas sp.

et al. 2020

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Fluorescent markers are a powerful tool and have been widely applied in biology for different purposes. The genome sequence of Xanthomonas citri subsp. citri (X. citri) revealed that approximately 30% of the genes encoded hypothetical proteins, some of which could play an important role in the success of plant-pathogen interaction and disease triggering. Therefore, revealing their functions is an important strategy to understand the bacterium pathways and mechanisms involved in plant-host interaction. The elucidation of protein function is not a trivial task, but the identification of the subcellular localization of a protein is key to understanding its function. We have constructed an integrative vector, pMAJIIc, under the control of the arabinose promoter, which allows the inducible expression of red fluorescent protein (mCherry) fusions in X. citri, suitable for subcellular localization of target proteins. Fluorescence microscopy was used to track the localization of VrpA protein, which was visualized surrounding the bacterial outer membrane, and the GyrB protein, which showed a diffused cytoplasmic localization, sometimes with dots accumulated near the cellular poles. The integration of the vector into the amy locus of X. citri did not affect bacterial virulence. The vector could be stably maintained in X. citri, and the disruption of the α-amylase gene provided an ease screening method for the selection of the transformant colonies. The results demonstrate that the mCherry-containing vector here described is a powerful tool for bacterial protein localization in cytoplasmic and periplasmic environments.

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Section: Introductionmentioning

confidence: 99%

“…X . citri genome was published many years ago [ 5 ], and since then a strong effort has been done to understand the molecular pathways used by this bacterium during the infection process [ 6 – 10 ]. The virulence of X .…”

Section: Introductionmentioning

confidence: 99%

mCherry fusions enable the subcellular localization of periplasmic and cytoplasmic proteins in Xanthomonas sp.

et al. 2020

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Biocontrol: A Sustainable Agricultural Solution for Management of Plant Diseases

Walia

Putatunda²,

Sharma

et al. 2021

Microbial Biotechnology in Crop Protection

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Closely-related Xanthomonas citri subsp. citri isolates trigger distinct histological and transcriptional responses in Citrus limon

Abstract: Citrus canker, caused by Xanthomonas citri subsp. citri (Xcc), has an important economic impact on the citrus industry. Extensive information is available about the disease but, nevertheless, the study of plant-pathogen interactions could provide new information in the un-

Cited by 2 publications

References 30 publications

mCherry fusions enable the subcellular localization of periplasmic and cytoplasmic proteins in Xanthomonas sp.

mCherry fusions enable the subcellular localization of periplasmic and cytoplasmic proteins in Xanthomonas sp.

Biocontrol: A Sustainable Agricultural Solution for Management of Plant Diseases

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