Growth of Burkholderia sacchari LFM 101 cultivated in glucose, sucrose and glycerol at different temperatures

Nascimento, Valkirea Matos; Silva, Luiziana Ferreira da; Gomez, José Gregório Cabrera; Fonseca, Gustavo Graciano

doi:10.1590/0103-9016-2015-0196

Cited by 13 publications

(14 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The production of PHB from sucrose is less common than from other carbon sources, such as glucose, fructose or glycerol, it was reported for some bacteria such as Burkholderia sacchari, Azotobacter vinelandii , Alcaligenes latus and Hydrogenophaga sp . (25·9, 66·0, 63 and 68·5 g per 100 g DCW, respectively ) (Jiang et al ; Nascimento et al ).…”

Section: Resultsmentioning

confidence: 99%

Forest soil bacteria able to produce homo and copolymers of polyhydroxyalkanoates from several pure and waste carbon sources

Clifton-García

González-Reynoso

Robledo‐Ortíz

et al. 2020

Lett Appl Microbiol

View full text Add to dashboard Cite

Two bacterial strains able to produce polyhydroxyalkanoates (PHAs) from a wide variety of pure carbon sources (dextrose, xylose, sucrose, lactose and glycerol) were isolated from forest soils and identified as Achromobacter mucicolens and Stenotrophomonas rhizophila. Achromobacter mucicolens also produced poly(3‐hydroxybutyrate) (PHB) from different wastes (cheese whey, molasses, agave bagasse hydrolysate, nejayote and mango waste pulp). Stenotrophomonas rhizophila, produced the copolymer poly(3‐hydroxybutyrate‐co‐3‐hydroxyvalerate) (PHB‐co‐HV) from glycerol (7·7 mol% of HV), and from sucrose with addition of propionic or valeric acid (4·5 and 25 mol% of HV, respectively). The copolymers presented a lower melting point (145, 156 and 127°C) and crystallinity (23, 26 and 16%) than PHB. The maximum biopolymer accumulation (PHB) for each strain growing in pure carbon source was as follows: 31·3 g per 100 g dry cell weight (DCW) for A. mucicolens from xylose; and 13·7 g per 100 g DCW for S. rhizophila from sucrose. Regarding the waste carbon sources, the highest PHB accumulation was obtained from agave bagasse hydrolysate (20·4 g per 100 g DCW) by A. mucicolens. The molecular weights of the biopolymers obtained ranged from 200 to 741 kDa. Significance and Impact of the Study The economic cost of the carbon source for the culture of polyhydroxyalkanoates (PHAs)‐producing microorganisms is one of the main process limitations. Therefore, it is vital to find versatile microorganisms able to grow and to accumulate homo and copolymers of PHAs from low‐cost substrates. In this research, we report two bacterial strains that produce poly(3‐hydroxybutyrate), poly(3‐hydroxybutyrate‐co‐3‐hydroxyvalerate) or both from at least five pure and five waste carbon sources. These results, by such bacterial strains have not been reported, especially the production of copolymer from glycerol without addition of precursors by Stenotrophomonas rhizophila and the production of PHB from xylose and agave bagasse hydrolysate by Achromobacter mucicolens.

show abstract

Section: Resultsmentioning

confidence: 99%

Forest soil bacteria able to produce homo and copolymers of polyhydroxyalkanoates from several pure and waste carbon sources

Clifton-García

González-Reynoso

Robledo‐Ortíz

et al. 2020

Lett Appl Microbiol

View full text Add to dashboard Cite

show abstract

“…Sugarcane sucrose is a low-cost alternative substrate (Nascimento et al, 2016). However, it is necessary the microalgae have the enzyme invertase for the hydrolysis of sucrose for subsequent glucose and fructose assimilation (Perez-Garcia et al, 2011).…”

Section: Resultsmentioning

confidence: 99%

“…The maximum biomass concentration was indicated by the maximum ABS 670 observed in each experiment. The measured absorbance values were also converted to cell concentration (X) values using a linear relation (optical density units per cell concentration) thus obtaining a conversion factor (Nascimento, Silva, Gomez, & Fonseca, 2016). Cell productivity (P x ) was calculated according to…”

Section: Methodsmentioning

confidence: 99%

<b>Screening of an adapted culture medium composed by different carbon sources for heterotrophic cultivation of <i>Chlorella vulgaris</i> using a microplate assay

Silva

Fonseca

2018

Acta Sci. Biol. Sci.

Self Cite

View full text Add to dashboard Cite

The ability of microalgae to grow heterotrophically lies in the ability to oxidize organic compounds present in the external environment. The aim of this work was to evaluate the growth of Chlorella vulgaris under heterotrophic culture conditions using Basal Bold and NPK media supplemented with different sole carbon sources (glucose, fructose, sucrose, glycerol or acetate). The kinetic parameters obtained were maximum specific growth rate (μ max), doubling time (DT), maximal absorbance, which was also converted to cell concentration values using a linear relation, and cell productivity (P X). Among all the treatments analyzed, the highest maximum specific growth rate found was 0.030 hour-1 (0.72 day-1) in the treatment using Basal Bold medium supplemented with glucose. The highest cellular concentration and cell productivity were also found for this same treatment (4.03 x 10 6 cell mL-1 and 64.0 x 10 6 cell L-1 day-1 , respectively). It was concluded that that the Basal Bold medium was more efficient for Chlorella vulgaris growth, since it induced higher values of μ max and cellular concentration. Results obtained were very reproducible using microplate assay.

show abstract

“…Growth was expressed in terms of microbial population (CFU/g) during cultivation. The maximum specific growth rate (µ max ) was determined as the slope of this linear region in the exponential growth phase [18]. Mesophilic bacteria were determined by using plate counting agar (PCA) in deep (35 °C, 48 h) and psychrotrophic bacteria using PCA in surface (7 °C, 240 h) [19] to monitor possible contamination during experiments.…”

Section: Determination Of Lichtheimia Ramosamentioning

confidence: 99%

Biotransformation of fruit residues via solid state bioprocess using Lichtheimia ramosa

Silva

et al. 2020

SN Appl. Sci.

Self Cite

View full text Add to dashboard Cite

Lichtheimia ramosa is a promising candidate fungus for solid state bioprocesses (SSB) due its rapid colonization. Fruit wastes present ideal conditions for fungal growth and biotransformation, which can promote the release of products of biotechnological interest. Thus, the aim of this work was to evaluate the mycelial growth and the enzymatic activities of L. ramosa in pineapple (Ananas comosus), orange (Citrus sinensis), mango (Mangifera indica), passion fruit (Passiflora edulis) and grape (Vitis vinifera) fruit wastes via SSB, and their influence in the generation of molecules with potential use. The SSB was evaluated in terms of capacity of biotransformation of the substrate (composition, protein enrichment, and fatty acid profile) and production of amylase, carboxymethylcellulase (CMCase), xylanase and lipase enzymes. Main cultivations were carried out at 30 °C for 30 days and every 5 days samples were taken and analyzed for microbiological content, proximal composition and enzymatic profile. Fatty acids were determined at day 0 (baseline) and at the end of the cultivations. L. ramosa grew well in all substrates up to the 25th day, except for on the orange residue, upon which development was slightly lower at this time. Protein enrichment was found in all substrates as follows: passion fruit (309.54%), pineapple (294.89%), mango (263.45%), orange (65.60%) and grapes (19.17%). Regarding enzymes, lipase was not synthetized in any of the substrates, though amylase, CMCase and xylanase were observed at different levels. The fatty acid profile varied from raw to cultivated substrates, indicating that L. ramosa can act in the synthesis and conversion of these acids. It was concluded that L. ramosa and the studied substrates are viable for SSB.

show abstract

Growth of Burkholderia sacchari LFM 101 cultivated in glucose, sucrose and glycerol at different temperatures

Cited by 13 publications

References 14 publications

Forest soil bacteria able to produce homo and copolymers of polyhydroxyalkanoates from several pure and waste carbon sources

Forest soil bacteria able to produce homo and copolymers of polyhydroxyalkanoates from several pure and waste carbon sources

<b>Screening of an adapted culture medium composed by different carbon sources for heterotrophic cultivation of <i>Chlorella vulgaris</i> using a microplate assay

Biotransformation of fruit residues via solid state bioprocess using Lichtheimia ramosa

Contact Info

Product

Resources

About