Extraction, characterization and microencapsulation of isoflavones from soybean molasses

Batista, Vanessa Sabrina Fagundes; Nunes, Guilherme S.; Viegas, Gabriela Ilha; Lucas, Bruna Nichelle; Bochi, Vivian Caetano; Emanuelli, Tatiana; Barin, Juliano Smanioto; Menezes, Cristiano Ragagnin de; Rosa, Cláudia Severo da

doi:10.1590/0103-8478cr20190341

Cited by 9 publications

(5 citation statements)

References 15 publications

(14 reference statements)

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“…Our high values can be attributed to some modifications in the extraction methods, concentrating the extract, and therefore the microcapsules presented a greater antioxidant capacity. In other studies of the microencapsulation of bioactive compounds, Batista et al [34] microencapsulated soy molasses isoflavones by spray drying using similar conditions to our research (Maltodextrin 18%, input temperature 140 • C), determining in the microcapsules an antioxidant capacity of 22.74 µmol TE/g through the ORAC assay, this value was much lower than that reported in oregano microcapsules. Additionally, Ydjeed et al [35] encapsulated phenolic compounds from Carob (Ceratonia siliqua L.) pulp extracts, evaluating ORAC, FRAP, and DPPH • capacity, reporting 295.8, 156.1, and 309.7 µmol TE/g, respectively, values below those obtained in the reported oregano microcapsules.…”

Section: Antioxidant Capacity By Abts •+ Frap and Orac Assay In Oreg...supporting

confidence: 64%

Green Extracts and UPLC-TQS-MS/MS Profiling of Flavonoids from Mexican Oregano (Lippia graveolens) Using Natural Deep Eutectic Solvents/Ultrasound-Assisted and Supercritical Fluids

Bernal-Millán

Carrasco-Portugal

Heredia

et al. 2023

Plants

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Mexican oregano (Lippia graveolens) is an important source of bioactive compounds, such as flavonoids. These have presented different therapeutic properties, including antioxidant and anti-inflammatory; however, their functionality is related to the quantity and type of compounds, and these characteristics depend on the extraction method used. This study aimed to compare different extraction procedures to identify and quantify flavonoids from oregano (Lippia graveolens). Emerging and conventional technologies include maceration with methanol and water, and ultrasound-assisted extraction (UAE) using deep eutectic solvents (DES) such as choline chloride-ethylene glycol, choline chloride-glycerol, and choline chloride-lactic acid. Supercritical fluid extraction using CO2 as a solvent was also studied. Six different extracts were obtained and the total reducing capacity, total flavonoid content, and antioxidant capacity by ABTS•+, DPPH•, FRAP, and ORAC were evaluated. In addition, flavonoids were identified and quantified by UPLC-TQS-MS/MS. Results showed that UAE-DES had the best extraction effect and antioxidant capacity using colorimetric methods. However, maceration-methanol was superior in compound content, and highlighting naringenin and phloridzin were the major compounds. In addition, this extract was microencapsulated by spray drying, which provided a protection feature of their antioxidant potential. Oregano extracts are rich in flavonoids and the microcapsules present promising results for future research.

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Section: Antioxidant Capacity By Abts •+ Frap and Orac Assay In Oreg...supporting

confidence: 64%

Green Extracts and UPLC-TQS-MS/MS Profiling of Flavonoids from Mexican Oregano (Lippia graveolens) Using Natural Deep Eutectic Solvents/Ultrasound-Assisted and Supercritical Fluids

Bernal-Millán

Carrasco-Portugal

Heredia

et al. 2023

Plants

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“…Soy ( glycine max ) and kudzu roots (KR) ( Pueraria spp.) are phytoestrogenic botanical sources that contain high amounts of naturally occurring polyphenols, namely isoflavones [ 13 , 14 , 15 ]. Accumulating evidence has substantially confirmed the fundamental health benefits of the consumption of these isoflavones.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant and Cytotoxic Activities of Kudzu Roots and Soy Molasses against Pediatric Tumors and Phytochemical Analysis of Isoflavones Using HPLC-DAD-ESI-HRMS

Aboushanab

Шевырин

Slesarev

et al. 2022

Plants

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Pediatric solid tumors (PSTs) are life-threatening and can lead to high morbidity and mortality rates in children. Developing novel remedies to treat these tumors, such as glioblastoma multiforme and sarcomas, such as osteosarcoma, and rhabdomyosarcoma, is challenging, despite immense attempts with chemotherapeutic or radiotherapeutic interventions. Soy (Glycine max) and kudzu roots (KR) (Pueraria spp.) are well-known phytoestrogenic botanical sources that contain high amounts of naturally occurring isoflavones. In the present study, we investigated the antioxidant and cytotoxic effects of the extracts of KR and soy molasses (SM) against PSTs. The green extraction of isoflavones from KR and SM was performed using natural deep eutectic solvents. The extracts were subsequently analyzed by high-performance liquid chromatography (HPLC)-diode array detector (DAD) coupled with high-resolution (HR) mass spectrometry (MS), which identified 10 isoflavones in KR extracts and 3 isoflavones in the SM extracts. Antioxidant and cytotoxic activities of KR and SM extracts were assessed against glioblastoma multiforme (A-172), osteosarcoma (HOS), and rhabdomyosarcoma (Rd) cancer cell lines. The KR and SM extracts showed satisfactory cytotoxic effects (IC50) against the cancer cell lines tested, particularly against Rd cancer cell lines, in a dose-dependent manner. Antioxidant activity was found to be significantly (p ≤ 0.05) higher in KR than in SM, which was consistent with the results of the cytotoxic activity observed with KR and SM extracts against glioblastoma and osteosarcoma cells. The total flavonoid content and antioxidant activities of the extracts were remarkably attributed to the isoflavone content in the KR and SM extracts. This study provides experimental evidence that HPLC-ESI-HRMS is a suitable analytical approach to identify isoflavones that exhibit potent antioxidant and anticancer potential against tumor cells, and that KR and SM, containing many isoflavones, can be a potential alternative for health care in the food and pharmaceutical industries.

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“…Because of the environmental impacts associated with the generation of by-products during the processing of soy-based food, there is a desperate need to recycle this waste in order to reduce the production costs and maximize the overall use of food [12]. In general, soy molasses, a dark brown syrup rich in carbohydrates and isoflavones, is produced concomitantly with the concentrate of soy protein [13].…”

Section: Introductionmentioning

confidence: 99%

Phytochemical screening and properties of botanical crude extracts and ethyl acetate fractions isolated by deep eutectic solvent

et al. 2022

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Botanicals, being safe because of their natural origin, are associated with potential health benefits due to their isoflavones-rich content. Isoflavones as plant-generated secondary metabolites are formed to defend against environmental stress such as UV radiation, mechanical damage, or any other attack from the surroundings. Kudzu (Pueraria roots and flowers), red clover (Trifolium pratense), and soy (Glycine max) are of significant importance in the medical field. Isoflavones were reported to exhibit a variety of biological functions, e.g., antiaging, anticancer, and microbiome modulations. This study focuses attention on the extraction and quantification of isoflavones from kudzu roots (KR), kudzu flowers (KF), red clover (RC), and soy molasses (SM), and determining their total polyphenols (TPC), flavonoids (TFC), and antioxidant activity (AOA). Novel green ultrasound-assisted extraction (UAE) technology was developed to extract and quantify the isoflavones. The results showed the quantity of isoflavones in KR, KF, RC, and SM crude extracts to be 1.01%, 2.9%, 0.70% and 0.67%, respectively. The fractionation using ethyl acetate (EA) improved the yield that became 1.48%, 2.5%, 1.3% and 0.89% in the KR, RC and SM extract fractions, respectively. This investigation eventually showed that our green UAE methodology is a safe and efficient method for recovering isoflavones. The total isoflavone contents were recorded as follows: KFKRRCSM. We concluded that these botanicals are a crucial source of isoflavones that exhibit strong antioxidant properties.

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Extraction, characterization and microencapsulation of isoflavones from soybean molasses

Cited by 9 publications

References 15 publications

Green Extracts and UPLC-TQS-MS/MS Profiling of Flavonoids from Mexican Oregano (Lippia graveolens) Using Natural Deep Eutectic Solvents/Ultrasound-Assisted and Supercritical Fluids

Green Extracts and UPLC-TQS-MS/MS Profiling of Flavonoids from Mexican Oregano (Lippia graveolens) Using Natural Deep Eutectic Solvents/Ultrasound-Assisted and Supercritical Fluids

Antioxidant and Cytotoxic Activities of Kudzu Roots and Soy Molasses against Pediatric Tumors and Phytochemical Analysis of Isoflavones Using HPLC-DAD-ESI-HRMS

Phytochemical screening and properties of botanical crude extracts and ethyl acetate fractions isolated by deep eutectic solvent

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