Culture medium and inoculation methodology for the study of soft root rot caused by Phytopythium sp.

Silva, Jonny Lucio de Sousa; Ishida, A. K. N.; Cunha, Rosiane Lopes da; Lima, Aline Medeiros; Cunha, E. F. M.

doi:10.1590/0103-8478cr20180875

Cited by 2 publications

(1 citation statement)

References 10 publications

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“…An example of this may be the study by Boari et al (2018), who analyzed the causes of soft rot of manioc roots (Manihot esculenta). This disease in Brazil is considered the greatest threat to the cultivation of this shrub (Poltronieri et al 1996;Silva et al 2019). At that time, a single organism responsible for the occurrence of the symptoms of this disease has not been diagnosed, but rather it is considered to be related to the action of many soil organisms (Hillocks and Wydra 2002).…”

Section: And Forestrymentioning

confidence: 99%

Phytopythium: origin, differences and meaning in modern plant pathology

Tkaczyk

2020

Folia Forestalia Polonica

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The genus Phytopythium is a relatively new group of organisms distinguished from the Pythium genus. These are organisms closely related to Pythium and Phytophthora, with similar structure and biology. Despite many similarities, this genus is characterised by several morphological features that allow it to be easily identified. Until now, more than 20 species belong to this genus, most of which are saprophytic organisms. However, there is also a group of species particularly dangerous to plants. These species include, among others, Pp. litorale, Pp. helicoides and Pp. vexans. The purpose of this work was to collect information about these organisms and present them in a condensed form. The study contains basic information about the history of the type of separation, differences in morphology distinctive genus of Phytopythium, Phytophthora and Pythium and information about diseases and host plants. Information is also provided on the potential threat posed by these organisms to forest ecosystems.

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Section: And Forestrymentioning

confidence: 99%

Phytopythium: origin, differences and meaning in modern plant pathology

Tkaczyk

2020

Folia Forestalia Polonica

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Influence of Culture Media and Environmental Factors (Water Potential and Temperature) on Mycelial Growth of Phytopythium vexans (de Bary), the Causal Agent of Dieback Disease in Apple Trees

Jabiri

Hamss

Amraoui

et al. 2022

Applied Microbiology

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This study aimed at evaluating the effects of culture media and environmental factors (temperature and water potential (Ψw)) on the growth of the pathogenic fungus Phytopythium vexans (de Bary) associated with root rot and dieback disease in apple trees. Tomato agar, potato dextrose agar (PDA), and soybean agar were the most favourable for rapid mycelial growth, with optimum growth recorded for PDA medium. To determine the environmental conditions that promoted the development of this phytopathogen, the effects of temperature (5–30 °C), water potential (Ψw) (−15.54; −0.67 MPa) (0.89–0.995 aw), and their interaction were evaluated on the in vitro radial growth rates of the five isolates of P. vexans and on their latency phase (time period prior to growth). The results of this study showed that temperature, water potential, and their interaction had significant effects (p < 0.001) on the radial growth rates and latency phases of all tested P. vexans isolates. All isolates were able to grow throughout the temperature range (5 to 30 °C), with the maximum radial growth rate being observed at the highest temperatures, 25–30 °C. Growth was seen to be faster at −0.67 MPa (0.995 aw) at 25 °C and 30 °C. No growth was observed at Ψw < −5.44 MPa (0.96 aw), regardless of the temperature. It was found that the length of the latency phase depended significantly on both environmental factors. The longest latency phases (5 days on average) were recorded at a temperature of 5 °C and Ψw of −0.67 MPa (0.995 aw) and −2.69 MPa (0.98 aw), while the shortest latency phases were observed at a temperature of 30 °C and a Ψw of −0.67 MPa (0.995 aw), with an average of 0.2 days. The findings from this study could help to understand the impact of these environmental factors on the occurrence of diseases caused by P. vexans and more likely to design a reliable preventive control strategy based on the avoidance of conditions that play in favour of the phytopathogen.

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Culture medium and inoculation methodology for the study of soft root rot caused by Phytopythium sp.

Cited by 2 publications

References 10 publications

Phytopythium: origin, differences and meaning in modern plant pathology

Phytopythium: origin, differences and meaning in modern plant pathology

Influence of Culture Media and Environmental Factors (Water Potential and Temperature) on Mycelial Growth of Phytopythium vexans (de Bary), the Causal Agent of Dieback Disease in Apple Trees

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