Adapted Bailenger method improves the rate of Ascaris suum eggs recovery from liquid pig manure compost

Abstract

Liquid pig manure (LPM) is widely used as a compost fertilizer for vegetable crops destined for human consumption. However, these wastes may contain parasites eggs, such as the nematode Ascaris suum, that pose serious health risks to humans. We attempted to determine the most appropriate technique for recovering A. suum eggs from LPM compost. Samples were collected from two waste sources during composting, including 23 samples containing LPM, sawdust, and wood shavings, and 14 samples of LPM alone-both in triplicate. Samples were analyzed using several different recovery methods. Recovery of eggs by the modified Bailenger method with adaptations was significantly more effective and recovered 57% more eggs than by the modified Bailenger method alone. Willis-Mollay method, modified Faust method, and the simple sedimentation technique only recovered 4.4%, 13.9%, and 26% of eggs, respectively, compared with the modified Bailenger method with adaptations, indicating that the adjustments made to the Bailenger method were key to improving the recovery of A. suum eggs from compost and LPM.

Detection of Helminth Ova in Wastewater Using Recombinase Polymerase Amplification Coupled to Lateral Flow Strips

“…WHO recommends an upper limit of one helminth ova per litre for recycled water to be judged suitable for irrigation and public use [2,21,22]. Based on such recommendations, the modified Bailenger method was suggested as a universal method for the detection of one helminth ova per 10 litres of recycled water for urban, agricultural, industrial, or environmental use [23,24]. However, the method has drawbacks, such as being time consuming (approximately three days), insensitive, and involves ova recovery, morphological identification, and enumeration of helminth ova [25][26][27].Diagnostic tests that are sensitive and specific are critical for monitoring Ascaris species in wastewater and sludge [28][29][30].…”