Morphological, biochemical, and molecular characterization of Meloidogyne spp. populations from Brazilian soybean production regions

Abstract

Soybean is a commodity of great economic importance worldwide, particularly in Brazil, world’s second largest producer. Nematodes, especially those of the Meloidogyne genus, severely limit productivity. Identification of nematode species is important for effective soybean management. Here, 26 populations of root-knot nematode (Meloidogyne spp.) from 15 municipalities in the states of Bahia, Mato Grosso, Goias, and Minas Gerais were characterized based on the morphology of the female perineal region, esterase profile, and identification based on amplification of specific regions of the population genome. Among the Meloidogyne spp. populations obtained, M. incognita and M. javanica, were identified. No mixed populations were present in the samples. Diagnosis based on molecular analysis was shown to be reliable and the fastest for characterization of nematode populations compared to other methods analyzed.

Response of green manure species and millet cultivars to different populations of Meloidogyne javanica

“…Meloidogyne javanica (Treub, 1885) Chitwood (Tylenchida: Heteroderidae) is one of the most important plant-parasitic nematodes because of its wide host range and geographical distribution. This pathogen can substantially limit the yield of grains, vegetables and fruits (Oliveira et al, 2017). In soybean, M. javanica can cause losses of up to 30% (Dias et al, 2010).…”

“…A complex interaction occurs between plant and pathogen. The establishment of nematode feeding sites in the host root system causes cell hyperplasia and hypertrophy (Ferraz and Brown, 2016), leading to gall formation in roots, wilting during the hottest hours of the day and leaf chlorosis (Oliveira et al, 2017).…”

Bioactivity of Schinus molle L. and Schinus terebinthifolia Raddi. Essential Oils on Anticarsia gemmatalis (Hübner 1818)

“…Soybean cultivation represents the main crop for Brazilian agribusiness and has shown significant growth in the last decades due to advances in technology [2]. The yield in the 2018/2019 harvest was 3,189 kg/ha, with a total production of more than 113 million of tons [3].…”