Correlation between systolic function and presence of myofibroblasts in doxorubicin-induced cardiomyopathy

Gava, F. N.; Silva, Sheila Nogueira Saraiva da; Rosa, Fernando Azadinho; Ortiz, E. M. G.; Rodrigues, Bruno; Bandarra, Márcio de Barros; Vasconcelos, Rosemeri de Oliveira; Camacho, Aparecido Antônio

doi:10.1590/0103-8478cr20151062

Cited by 1 publication

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References 16 publications

(14 reference statements)

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“…In agreement with Gava et al . [32] , the present work showed the only cells expressed α-SMA in the myocardium of control group were vascular smooth muscle cells. However two weeks AZ treatment induced significant increase in α-SMA immunostained elongated cells among the cardiomyocytes indicated intense proliferation of myofibroblasts.…”

Section: Discussionsupporting

confidence: 58%

“…Under pathological conditions, these fibroblasts are transformed into myofibroblasts which are responsible for excessive production of fibronectin and collagen resulted in myocardial fibrosis. Since myofibroblasts exhibited characteristics of fibroblasts and smooth muscle cells, their presence in the myocardium can be assessed by immunohistochemical detection of alpha smooth muscle actin (α-SMA) [32] .…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of Azithromycin Induced Cardiotoxicity in Male Albino Rats and the Possible Protective Role of Nigella Sativa Oil

El-kader

2019

Egyptian Journal of Histology

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Background: Azithromycin (AZ) is a broad spectrum macrolide antibiotic frequently used in treatment of bacterial infections. There are many cardiac adverse effects asso¬ciated with AZ treatment according to case reports and cohort studies. However, there are only few published experimental studies reported its cardiotoxicity in rats. Aim of the Work:The present work aimed to study the histopathological changes in the heart of male albino rat induced by AZ treatment. Also, to clarify the underlying mechanisms regarding oxidative stress, inflammatory marker release, apoptotic cell-death and myocardial fibrosis. Finally, the supposed protective effects of nigella sativa oil (NSO) co-treatment were assessed. Material and Methods: Twenty four adult male albino rats were divided into equal 4 groups; Control, AZ (30 mg/ kg/ day), AZ (30 mg/ kg/ day) +NSO (4 ml/ kg/ day), and NSO (4 ml/ kg/ day). The drugs were administrated intragastrically once daily for 2 weeks. Rats were sacrificed, blood and tissue samples were collected and processed for biochemical, histolopathological and immunohistochemical studies. Results: AZ treated group showed marked elevation in creatine phosphokinase (CPK), lactate dehydrogenase (LDH), Malondialdehyde (MDA), and tumor necrosis factor alpha (TNFα) levels. It induced significant myocardial necrosis, fibrosis, and apoptosis. It was the first time to demonstrate the effect of AZ on myofibroblasts proliferation by evaluating alpha smooth muscle actin (α-SMA) immunohitochemical expression which revealed significant increase after AZ treatment. Co-treatment with NSO significantly lowered CPK, LDH, MDA, and TNFα levels, preserved the cardiac morphology, and decreased Caspase-3 and αSMA immunoreactivity as compared to AZ group. Conclusion: Concluded that AZ induced cardiac adverse side effect in rats. NSO could prevent AZ-induced cardiotoxicity, and its mechanism may be related to antioxidant, antiinflammatory, antiapoptotic and antifibrotic properties. Further studies are required to confirm the efficacy of NSO as a protective agent in human AZ intoxication.

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Section: Discussionsupporting

confidence: 58%

Section: Discussionmentioning

confidence: 99%

Evaluation of Azithromycin Induced Cardiotoxicity in Male Albino Rats and the Possible Protective Role of Nigella Sativa Oil

El-kader

2019

Egyptian Journal of Histology

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Correlation between systolic function and presence of myofibroblasts in doxorubicin-induced cardiomyopathy

Abstract: Cardiotoxicity induced by doroxubicin generates systolic disfunction and myocardial remodeling with presence of myofibroblasts. These cells are thought to be attracted to the injured heart to avoid the development of congestive

Cited by 1 publication

References 16 publications

Evaluation of Azithromycin Induced Cardiotoxicity in Male Albino Rats and the Possible Protective Role of Nigella Sativa Oil

Evaluation of Azithromycin Induced Cardiotoxicity in Male Albino Rats and the Possible Protective Role of Nigella Sativa Oil

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