Endogenous losses and true digestibility of phosphorus in rice bran with or without phytase determined with piglets

Lucca, Walter; Alves, Débora Aline; Rocha, Leonardo Tombesi da; Shirmann, Graciele Dalise; Camargo, Claudiele Aparecida do Santos; Figueiredo, Andriele Medianeira; Zanella, Irineo; Oliveira, Vladimir de

doi:10.1590/0103-8478cr20150801

Cited by 2 publications

(3 citation statements)

References 12 publications

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“…The majority of dietary phosphate in animal feed is present as phytate, the mixed metal salt of phytic acid [ myo -inositol 1,2,3,4,5,6-hexakisphosphate, InsP 6 ], the primary storage form of phosphate in plant tissues, but a form with reduced availability for digestion by non-ruminant animals [ 1 , 2 ]. In order to circumvent the need for the addition of inorganic rock phosphate and to reduce costs to the producer and consumer, poultry diets are now often supplemented with microbial phytase [ 3 ] with the intention of accessing phytate-bound phosphate as an economical, environmentally sustainable phosphate source.…”

Section: Introductionmentioning

confidence: 99%

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Phytase dose-dependent response of kidney inositol phosphate levels in poultry

Sprigg

Whitfield²,

Burton³

et al. 2022

PLoS ONE

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Phytases, enzymes that degrade phytate present in feedstuffs, are widely added to the diets of monogastric animals. Many studies have correlated phytase addition with improved animal productivity and a subset of these have sought to correlate animal performance with phytase-mediated generation of inositol phosphates in different parts of the gastro-intestinal tract or with release of inositol or of phosphate, the absorbable products of phytate degradation. Remarkably, the effect of dietary phytase on tissue inositol phosphates has not been studied. The objective of this study was to determine effect of phytase supplementation on liver and kidney myo-inositol and myo-inositol phosphates in broiler chickens. For this, methods were developed to measure inositol phosphates in chicken tissues. The study comprised wheat/soy-based diets containing one of three levels of phytase (0, 500 and 6,000 FTU/kg of modified E. coli 6-phytase). Diets were provided to broilers for 21 D and on day 21 digesta were collected from the gizzard and ileum. Liver and kidney tissue were harvested. Myo-inositol and inositol phosphates were measured in diet, digesta, liver and kidney. Gizzard and ileal content inositol was increased progressively, and total inositol phosphates reduced progressively, by phytase supplementation. The predominant higher inositol phosphates detected in tissues, D-and/or L-Ins(3,4,5,6)P4 and Ins(1,3,4,5,6)P5, differed from those (D-and/or L-Ins(1,2,3,4)P4, D-and/or L-Ins(1,2,5,6)P4, Ins(1,2,3,4,6)P5, D-and/or L-Ins(1,2,3,4,5)P5 and D-and/or L-Ins(1,2,4,5,6)P5) generated from phytate (InsP6) degradation by E. coli 6-phytase or endogenous feed phytase, suggesting tissue inositol phosphates are not the result of direct absorption. Kidney inositol phosphates were reduced progressively by phytase supplementation. These data suggest that tissue inositol phosphate concentrations can be influenced by dietary phytase inclusion rate and that such effects are tissue specific, though the consequences for physiology of such changes have yet to be elucidated.

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Section: Introductionmentioning

confidence: 99%

“…InsP, total InsP 2 to InsP 6 ; InsP 6 , inositol hexakisphosphate; InsP 5 , inositol pentakisphosphate; InsP 4 , inositol tetrakisphosphate; InsP 3 , inositol trisphosphate; InsP 2 , inositol bisphosphate 1. The control group was fed with a diet with 0.45% calculated available phosphate.…”

mentioning

confidence: 99%

Phytase dose-dependent response of kidney inositol phosphate levels in poultry

Sprigg

Whitfield²,

Burton³

et al. 2022

PLoS ONE

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“…Moreover, two-thirds of the phosphorus (P) present in RB is available as phytate-P [ 12 ]. It is often around 80% in many cases and unavailable in poultry and pigs due to a lack of phytase in the digestion process [ 13 ]. Therefore, a large portion of the dietary P of RB cannot be utilized and is excreted in the feces.…”

Section: Introductionmentioning

confidence: 99%

Anaerobic fermentation of rice bran using rumen liquor for desirable chemical changes as animal feed

Islam¹,

Elsabagh²,

Lv³

et al. 2022

J Adv Vet Anim Res

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Objective: The objectives of this research are to overcome the limitations of rice bran (RB) and de-oiled rice bran (DORB) by fermentation anaerobically using inoculum from the rumen of a canulated sheep for desirable chemical changes. Materials and Methods: Initially, RB and DORB were fermented by 10% rumen liquor for 12 h at 39°C at different moisture levels (10, 20, 30, 40, 50, and 60% phosphate buffer). Again, DORB was fermented for 24, 48, and 72 h at 39°C using 10% rumen liquor at different moisture levels (10, 20, 30, 40, 50, and 60% phosphate buffer). Before and after fermentation, RB and DORB were analyzed for pH, proximate components, neutral detergent fiber (NDF), total-P, inorganic-P, and phytate-P. Results: Fermentation of RB and DORB for 12 h reduced (p < 0.05) pH, crude fiber (CF),NDF, and phytate-P, but increased (p < 0.05) the content of inorganic-P. Subsequent fermentation of DORB for 24, 48, and 72 h reduced pH, CF, and NDF. Total-P of fermented DORB remained similar till 72 h fermentation (p < 0.05). But, inorganic-P increased with the increasing duration (24, 48, and 72 h) of fermentation and increased (30, 40, and 50) moisture level (p < 0.05). Alternatively, phytate-P decreased with increasing duration and moisture level (p < 0.05). Conclusion: Inoculation of rumen microbes and incubation of RB (12 h) and DORB (24 h) at room temperature reduced phytate-P and fiber content (CF and NDF) when the moisture level was up to 50%; those are the indicators to reduce the limitation of RB and DORB to use as feed for non-ruminant animals like poultry and pigs.

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Endogenous losses and true digestibility of phosphorus in rice bran with or without phytase determined with piglets

Abstract: The aim of this study was to determine endogenous losses and true total digestibility of phosphorus (P)

Cited by 2 publications

References 12 publications

Phytase dose-dependent response of kidney inositol phosphate levels in poultry

Phytase dose-dependent response of kidney inositol phosphate levels in poultry

Anaerobic fermentation of rice bran using rumen liquor for desirable chemical changes as animal feed

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