Biostimulants are used as a strategy to minimize the effects of climatic adversities by allowing the seedlings to express more strongly their metabolic capacity and to have a greater root system development. The objective was to evaluate the effect of seed treatment with different doses of a biostimulant composed of Ascophyllum nodosum on the agronomic performance of maize seedlings. The experiment was carried out in a randomized block design with five treatments and four replicates, using five doses of the biostimulant, 0,0; 0.25; 0.50; 0.75; and 1.00 liter of product per 100 kg of seeds. The following were evaluated: vigor, fresh mass of roots and shoots, dry mass of roots and shoots, root length, shoot height, and leaf area. The data were submitted to ANOVA and regression analysis to verify the behavior of the characteristics as a function of the biostimulant doses. In the treatment of seeds, these doses provided increases in vigor, shoot height, fresh and dry mass of shoots, and leaf area. The biostimulant derived from the Ascophyllum nodosum extract showed potential use in the treatment of maize seeds, with the doses of 0.50 and 0.75 liters per 100 kg seeds being the most promising for the initial development of maize seedlings.