Uncaria tomentosa and  Uncaria guianensis an agronomic history to be written

Honório, Isabela Cristina Gomes; Bertoni, Bianca Waléria; Pereira, Ana Maria Soares

doi:10.1590/0103-8478cr20150138

Cited by 18 publications

(20 citation statements)

References 31 publications

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“…tomentosa , has compromised the survival of the species in the wild [7]. To date, there have been no reports concerning the conservation of natural populations of U .…”

Section: Introductionmentioning

confidence: 99%

Genetic and chemical diversity of Uncaria tomentosa (Willd. ex. Schult.) DC. in the Brazilian Amazon

et al. 2017

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Uncaria tomentosa (Willd. ex Schult.) DC., a plant native to the Amazon region, is used widely in popular medicine and by the pharmaceutical industry because of its anti-inflammatory activity. However, the survival of this species is endangered by deforestation and indiscriminate collection, and a preservation plan is urgently required. The objectives of this study were to determine the genetic and chemical variability between and within eight populations of U. tomentosa from the Brazilian states of Acre, Pará and Amapá, and to investigate possible correlations between genetic and geographical distances, and between geographical distances or altitude and the accumulation of bioactive oxindole alkaloids. Three sequence-related amplified polymorphism (SRAP) markers were employed to fingerprint genomic DNA, and the amounts of mitraphylline and isomitraphylline in leaf samples were established by high-performance liquid chromatography. Although significant divergence existed between the tested populations (FST = 0.246), the largest genetic diversity and the highest percentage of polymorphism (95.68%) was found within the population from Mâncio Lima, Acre. Gene flow was considered rather limited (Nm = 1.57), and no correlations between genetic and geographical distances were detected, suggesting that population structure followed an island model. Accumulations of mitraphylline and isomitraphylline varied in the range 32.94 to 0.57 and 3.75 to 0.36 mg g-1 dry weight, respectively. The concentration of isomitraphylline was positively influenced by altitude, such that the population collected at the site with the highest elevation (Tarauacá, Acre) exhibited the greatest alkaloid content. SRAP markers were very efficient in fingerprinting genomic DNA from U. tomentosa populations and clearly showed that genetic variability within populations was greater than between populations. A conservation and management plan should prioritize the creation of germplasm banks to prevent the loss of existing genetic variability, particularly within alkaloid-rich populations such as those of Tarauacá.

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“…tomentosa , has compromised the survival of the species in the wild [7]. To date, there have been no reports concerning the conservation of natural populations of U .…”

Section: Introductionmentioning

confidence: 99%

Genetic and chemical diversity of Uncaria tomentosa (Willd. ex. Schult.) DC. in the Brazilian Amazon

et al. 2017

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“…guianensis (F st = 0.188) was high but slightly lower than that observed for U . tomentosa (F st = 0.246) [ 8 ]. However, this index can be very variable among species of the same family and/or genus that inhabit a common biome, as has been described for populations of the Amazonian medicinal plant Psychotria ipecacuanha (Rubiaceae), which presented high F st values ranging from 0.355 to 0.457 [ 24 ].…”

Section: Resultsmentioning

confidence: 99%

“…Natural populations of U . guianensis have declined dramatically in recent times because of strong anthropic pressure brought about mainly by deforestation and indiscriminate extraction of the bark for the commercial production of phytotherapeutic preparations [ 7 , 8 ]. In this context, studies on the genetic and chemical variability of medicinal plants are particularly important since they enable the selection of elite individuals that would be of interest to the pharmaceutical industry [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Genetic structure and chemical diversity in natural populations of Uncaria guianensis (Aubl.) J.F.Gmel. (Rubiaceae)

Honório¹,

Coppede²,

Delprete³

et al. 2018

PLoS ONE

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Uncaria guianensis is native to the Amazon and is used traditionally as an anti-inflammatory. Natural populations of the species have declined markedly in recent times because of strong anthropic pressure brought about by deforestation and indiscriminate collection. The aim of the present study was to assess the genetic and chemical diversity among eight natural populations of U. guianensis located in the Brazilian states of Acre, Amapá and Amazonas. A set of four primer combinations was employed in sequence-related amplified polymorphism (SRAP) amplifications of leaf DNA, and the fragments were analyzed in an LI-COR model 4300 DNA Analyzer. Genetic variability within the populations (81%) was substantially greater than that detected between them (19%). The highest percentage of polymorphic loci (90.21%) and the largest genetic variability were observed in the population located in Mazagão, Amapá. Genetic differentiation between populations was high (Fst = 0.188) and the studied populations formed three distinct genetic groups (K = 3). The population located in Assis Brasil, Acre, presented the highest average content of the mitraphylline (0.60 mg/g dry weight,). However, mitraphylline and isomitraphylline not detected in most individuals in the studied populations, and it is questionable whether they should be considered as chemical markers of the species. The genetic data confirm the urgent need for conservation programs for U. guianensis, and for further studies aimed at ascertaining the genetic basis and heritability of alkaloid accumulation.

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“…DC. is a woody vine native to Central and South Americas, mainly found in the Peruvian and Brazilian Amazon rainforest . The ethnomedicinal use of U. tomentosa by the Asháninka indigenous people is well documented.…”

Section: Introductionmentioning

confidence: 99%

Isolation of mitraphylline from Uncaria tomentosa (Willd. ex Schult.) DC. barks and development of spectrophotometric method for total alkaloids determination in Cat's Claw samples

Gouvêa

Pusceddu

Netto

et al. 2019

Phytochemical Analysis

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Introduction:The usual quality control for Uncaria tomentosa (Willd. ex Schult.) DC.barks requires highly specific analytical standards and methods based on highperformance liquid chromatography (HPLC), which impacts the costs of the analytical process and the final products.Objective: To obtain an analytical reference standard of mitraphylline by isolation from U. tomentosa barks and develop a spectrophotometric method for determination of total alkaloids in samples of U. tomentosa.Methodology: An alkaloid-enriched extract was obtained by acid-base partition and mitraphylline was selectively precipitated using an 80:20 v/v toluene/hexane solution. The compound was characterised by HPLC-UV/DAD (diode-array detector), mass spectrometry, UV-visible, infrared (IR) and 1 H-and 13 C-nuclear magnetic resonance (NMR) spectroscopy. Sample preparation for the spectrophotometric method consisted of an extraction with boiling methanol (3 × 10 mL, 15 min), followed by a strong cation exchange solid phase extraction (SCX-SPE) clean-up. Results: Mitraphylline with a purity of 98% was isolated in 0.05% m/m yield. All characterisation results were in agreement with previous published data. The spectrophotometric method showed linear range between 0.40 and 20 μg/mL; limits of detection and quantification of 0.15 and 0.49 μg/mg, respectively; dispersion of results lower than 5% for repeatability and intermediate precision; statistically proven accuracy by comparison with reference values obtained by Soxhlet and an HPLC-UV/ DAD method; and robustness in relation to sample mass extracted and extraction time. Conclusion:The methods developed to obtain mitraphylline analytical standard from U. tomentosa barks and to determine total alkaloids by spectrophotometry provided a cheaper and faster quality control alternative for U. tomentosa samples.

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Uncaria tomentosa and Uncaria guianensis an agronomic history to be written

Abstract: Uncaria tomentosa and

Cited by 18 publications

References 31 publications

Genetic and chemical diversity of Uncaria tomentosa (Willd. ex. Schult.) DC. in the Brazilian Amazon

Genetic and chemical diversity of Uncaria tomentosa (Willd. ex. Schult.) DC. in the Brazilian Amazon

Genetic structure and chemical diversity in natural populations of Uncaria guianensis (Aubl.) J.F.Gmel. (Rubiaceae)

Isolation of mitraphylline from Uncaria tomentosa (Willd. ex Schult.) DC. barks and development of spectrophotometric method for total alkaloids determination in Cat's Claw samples

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