Biological activity of Bt proteins expressed in different structures of transgenic corn against  Spodoptera frugiperda

Bernardi, Daniel; Bernardi, Oderlei; Horikoshi, Renato J.; Salmeron, Eloisa; Okuma, Daniela M.; Omoto, Celso

doi:10.1590/0103-8478cr20150047

Cited by 9 publications

(6 citation statements)

References 14 publications

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“…Some larvae fail to complete their life cycle due to toxicity of B. thuringiensis. Bernardi et al (2016) reported that 49% of treated S. frugiferda larvae could not complete their life cycle. Figure 1.…”

Section: Mortality=mentioning

confidence: 99%

The Use of Golden Snail Meal to Enrich Bacillus Thuringiensis Culture Media and Its Effect on the Bacterial Toxicity Against Spodoptera Litura

Pujiastuti

Masyitah²,

Dirgahayu³

et al. 2018

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA

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The use of golden snail meal to enrich Bacillus thuringiensis culture media and its effect on the bacterial toxicity against spodoptera litura. Bacillus thuringiensis is an entomopathogenic bacterium producing spore and protein at sporulation. There has been limited research on using golden snail as protein resource to enrich bacterial culture media. This research was aimed at studying bacterial cell/spore production in culture media made from coconut water media and liquid waste of tofu industry (tofu whey) enriched with golden snail meal (GSM), as well as its toxicity against Spodoptera litura. The research was conducted in the Laboratory of Entomology, Department of Plant Protection, Faculty of Agriculture, Sriwijaya University, from June to September 2017. The experiment was arranged in a Factorial Completely Randomized Design (FCRD) with two factors and three replications. The first factor was the addition of GSM (0, 5, 9, 13 and 17 g) and the second was storage longevity (0, 1, and 2 months). Number of test insect (third instar of S. litura) was 30 larvae per treatment. Parameter observed were spore density, larval mortality, and symptom of infected larvae. The results showed that B. thuringiensis cultured in culture media enriched with 13 g golden snail meal produced the highest spore density, amounted to 14.14 x 107spores/ml and caused larval mortality up to 86.67%. After one and two month storage treatments, the spore density in the cultures reduced to 2.51 x 107 and 1.49 x 107 spores/ml, respectively. There was a tendency of reduction in spore density under longer storage of the culture. Survived larvae developed abnormally, failed to transform to pupa or imago.

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Section: Mortality=mentioning

confidence: 99%

The Use of Golden Snail Meal to Enrich Bacillus Thuringiensis Culture Media and Its Effect on the Bacterial Toxicity Against Spodoptera Litura

Pujiastuti

Masyitah²,

Dirgahayu³

et al. 2018

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA

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“…Of the total area planted with corn and soybean in this country, 79% and 62% express Bt toxins, respectively (Agroconsult, 2018). In addition to corn and soybean, the Brazilian landscape also has cotton and sugarcane expressing Bt toxins, which, in addition to tropical conditions that provide ideal temperature and environmental conditions for insects to undergo multiple generations in a year and multiple plantings of Bt crops in a year, create a scenario favoring rapid resistance evolution (Bernardi et al, 2016; Fatoretto et al, 2017). Several Lepidoptera of economic importance have developed field‐evolved resistance to Bt toxins in Brazil, and major concerns are associated with feeding by fall armyworm, Spodoptera frugiperda (J.E.…”

Section: Introductionmentioning

confidence: 99%

“…In addition to biotic factors that favor resistance, Brazil has a different regulatory system for refuge compliance of regulations in other geographies (Carrière et al, 2020). In Brazil, the National Technical Committee for Biosafety (CTNBio) assesses the biosafety of modified crops, but it does not evaluate or regulate issues related to resistance management, and IRM is not mandatory (Bernardi et al, 2016; Carrière et al, 2020). Resistance management practices for Bt crops are promoted by technology providers and academic and government researchers (e.g., EMBRAPA) (CIB, 2018; IRAC, 2017).…”

Section: Introductionmentioning

confidence: 99%

How can policymakers and researchers develop effective insect resistance management guidelines? A quantitative and qualitative study of Brazilian farmers' perspectives and attitudes

Delborne

Reisig

2023

Plants People Planet

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Societal Impact StatementToday, over 80% of the US and Brazil row crop acreage has plants expressing insecticidal proteins to prevent the damage caused by caterpillars. These plants (crops expressing Bacillus thuringiensis, Bt, toxins) have brought several benefits to farmers, the environment, and society. However, these can be eroded when insects develop resistance to these toxins. Researchers and regulatory agencies have developed tactics that should be followed by farmers to avoid resistance but with limited efficacy. Our research provides recommendations for researchers and policymakers that are based on farmers' perspectives, thereby offering changes for current guidelines to successfully manage insect resistance and protect Bt crops' efficacy.Summary Genetically engineered crops expressing insecticidal proteins produced by Bacillus thuringiensis (Bt) have brought numerous benefits; however, pest resistance evolution poses a threat to the sustainability of this technology. Insect resistance management (IRM) for Bt crops has been defined as a wicked problem as it involves sociobiological complexities. A main challenge in IRM is the adoption of non‐Bt refuge, which is one out of the few strategies amenable to human intervention. This study investigated farmers' perspectives on information sources and IRM practices in Brazil using quantitative and qualitative data collection. A total of 145 farmers responded to online Qualtrics surveys, and 13 farmers participated in person to open‐ended interviews. This study demonstrates that farmers rely on strong social networks for information exchange and that sources with expertise based on local field experience are the most reliable channels of communication. We identified new challenges for refuge adoption such as the need to spray insecticides for pests not targeted by Bt and the intangible aspect of resistance evolution. Based on results of sources of information and perspectives on IRM practices, we discuss strategies that may be successful in delaying insecticide resistance evolution based on local contexts. This is the first study to investigate Brazilian farmers' perceptions on information sources and IRM strategies using qualitative data. Our results provide important elements to orient research development and decision‐making in biotechnology policies for the agricultural sector in Brazil and other similar contexts.

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“…Several populations of S. frugiperda that are resistant to synthetic insecticides (Almeida et al, 2017;Gutiérrez-Moreno et al, 2019;Boaventura et al, 2020), as well as to Bt corn, are used to control caterpillars (Bernardi et al, 2016;Boaventura et al, 2020). However, further researches are necessary to find other efficient ways for controlling that pest, in order to obtain acceptable and economical levels of agricultural yield in a sustainable way.…”

Section: Introductionmentioning

confidence: 99%

Application technology of SfMNPV-6nd in the control of fall armyworm

Dorneles

Raetano

Prado

et al. 2022

Pesq. agropec. bras.

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The objective of this work was to evaluate the application technology and the compatibility of the SfMNPV-6nd baculovirus with surfactants and markers in the mortality of Spodoptera frugiperda second-instar larvae. The compatibility studies were carried out with the brilliant blue (BB) dye and copper oxychloride (CO) markers and with the polyalkylene oxide heptamethyltrisiloxane (PH) and ethoxylated alkylphenol (EA) surfactants mixed with the SfMNPV-6nd biological insecticide. Droplet dispersion was assessed by spraying a SfMNPV-6nd solution on corn plants using flat fan and hollow cone nozzles. The evaluated parameters were: spray deposits, volumetric median diameter (VMD), droplet size, pH of spray solution, and mortality of second-instar S. frugiperda larvae. The PH and EA surfactants present synergism with SfMNPV-6nd and increase S. frugiperda control. The addition of the EA and CO surfactants to the SfMNPV-6nd solution reduces pH values. Droplet density, VMD, spray deposition of the SfMNPV-6nd solution, and mortality of S. frugiperda second-instar larvae do not differ among treatments regardless of the type of nozzle used.

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Biological activity of Bt proteins expressed in different structures of transgenic corn against Spodoptera frugiperda

Abstract: Spodoptera frugiperda (J. E. Smith) is the main target pest of Bt corn technologies, such as YieldGard VT PRO™ (Cry1A.105/Cry2Ab2) and PowerCore™ (Cry1A.105/Cry2Ab2/ Cry1F)

Cited by 9 publications

References 14 publications

The Use of Golden Snail Meal to Enrich Bacillus Thuringiensis Culture Media and Its Effect on the Bacterial Toxicity Against Spodoptera Litura

The Use of Golden Snail Meal to Enrich Bacillus Thuringiensis Culture Media and Its Effect on the Bacterial Toxicity Against Spodoptera Litura

How can policymakers and researchers develop effective insect resistance management guidelines? A quantitative and qualitative study of Brazilian farmers' perspectives and attitudes

Application technology of SfMNPV-6nd in the control of fall armyworm

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