Challenges and perspectives to enhance cattle production via in vitro techniques: focus on epigenetics and cell-secreted vesicles

Bressan, Fabiana Fernandes; Neto, Paulo Fantinato; Andrade, Gabriella Mamede; Sangalli, Juliano Rodrigues; Sampaio, Rafael Vilar; Silveira, Juliano Coelho da; Perecin, Felipe; Meirelles, Flávio Vieira

doi:10.1590/0103-8478cr20141294

Cited by 3 publications

(2 citation statements)

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“…However, further studies are needed to unravel the mechanisms in which the UH bearing the DF present lower levels of PGF2α when stimulated and focusing on blood flow and cell-to-cell communication features through extracellular vesicles might explain such modulation. Indeed, extracellular vesicles such as exosomes or microvesicles were already described in body fluid such as seminal plasma, uterine fluid and even follicular fluid (BURNS et al, 2014;TANNETTA et al, 2014;BRESSAN et al, 2015). These vesicles contain bioactive molecules such as RNAs, miRNAs and proteins that are able to mediate physiological processes.…”

Section: Discussionmentioning

confidence: 99%

Endometrial prostaglandin F2α in vitro production and its modulation regarding dominant follicle position in cattle

Bressan

Membrive

Goissis

et al. 2018

Braz. J. Vet. Res. Anim. Sci.

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Prostaglandin F2α (PGF2α) determines luteolysis in cattle, and the ability to manipulate its endogenous synthesis is indispensible for large-scale animal breeding. Estradiol (E 2 ) and progesterone (P 4 ) modulate several molecular pathways in endometrial cells, including the synthesis of PGF2α; however, its specific mechanisms are still not totally known. This study investigated the production in vitro and possible modulation of endometrial PGF2α due to a local effect of endogenous E 2 in the ipsilateral uterine horn (UH) containing the dominant follicle (DF) or from P 4 in ipsilateral horn containing the corpus luteum (CL). The PGF2α stimulators oxytocin (OT) and phorbol 12,13-dibutyrate (PDBu) were incubated with endometrial explants, and PGF2α content was measured. For that, cycling cows were synchronized, the development of DF and CL was examined by ultrasonography and on the seventh day of the estrous cycle, endometrial explants were collected and cultured in medium supplemented with 10 -6 M PDBu or 10 -6 M OT or non-supplemented. Media samples were collected immediately after treatment and 60 min later. Radioimmunoassay showed that the PGF2α content of the UH ipsilateral to the DF was 49% less than that of the contralateral UH (8.22 ± 0.95 vs. 12.24 ± 0.95 pg/mL/mg tissue, respectively; P < 0.01). However, the PGF2α levels did not differ between the UHs as a function of the CL position (9.46 ± 0.95 vs. 11 ± 0.95 pg/mL/mg; P > 0.05). The cellular stimulators promoted an increase in PGF2α synthesis (P < 0.02), and the effects differed among the animals (P < 0.04). The PGF2a production was higher in the explants treated with PDBu rather than OT (13.68 ± 1.16 vs. 10.01 ± 1.16 pg/mL/mg tissue, respectively; P < 0.05). In conclusion, PGF2α synthesis is modulated by the presence of the DF (local E 2 ) but not the CL (local P 4 ), and both PDBu and OT stimulated PGF2a synthesis.A prostaglandina F2α (PGF2α) determina a luteólise em bovinos. A capacidade de manipular sua síntese endógena é indispensável para a produção animal em grande escala. O estradiol (E 2 ) e a progesterona (P 4 ) modulam diversas vias moleculares das células endometriais, incluindo a síntese de PGF2α; no entanto, pouco se sabe sobre seus mecanismos específicos. Este trabalho investigou a produção in vitro e a possível modulação da PGF2α endometrial devido a um efeito local do E 2 endógeno no corno uterino ipsilateral ao folículo dominante (FD) ou da P 4 no corno ipsilateral ao corpo lúteo (CL). Os estimuladores de PGF2α oxitocina (OT) e 12,23-dibutirato de forbol (PDBu) foram incubados com explantes endometriais, e o conteúdo de PGF2α foi mensurado. Para tal, vacas cíclicas foram sincronizadas, o desenvolvimento de FD e CL foi examinado por ultrassonografia, e no 17º dia do ciclo estral os explantes endometriais foram coletados e cultivados em meio ou suplementados com PDBu 10 -6 M ou 10 -6 M OT. As amostras de meio foram coletadas imediatamente após o tratamento e sessenta minutos depois. O radioimunoensaio mostrou que o conteúdo de PGF2α do ...

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Section: Discussionmentioning

confidence: 99%

Endometrial prostaglandin F2α in vitro production and its modulation regarding dominant follicle position in cattle

Bressan

Membrive

Goissis

et al. 2018

Braz. J. Vet. Res. Anim. Sci.

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“…Despite advances in the field of somatic cell nuclear transfer (SCNT), especially in cattle, market growth continues to stumble due to the low efficiency of the cloning technique in producing viable offspring. Only 0.5% to 5% of reconstructed embryos produce term animals; this low success rate results in losses at the embryonic, fetal, and perinatal stages (Palmieri et al 2008, Bressan et al 2015, Ibtisham et al 2017. It has been estimated that up to 80% of losses occur in the second month of pregnancies in SCNT animals; in contrast, such losses occur in only one-quarter of pregnancies from in vitro fertilization (Wiltbank et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Pathological features of cloned calves that died in the neonatal period

et al. 2020

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Somatic-cell nuclear transfer is a cloning technique that enables the creation of a viable embryo from a donor adult to produce a genetically identical individual. This technique opens numerous potential possibilities for medicine and animal reproduction. However, several reports have documented cloning-related issues. Embryo and fetal losses remain significantly higher than in other techniques, and there is a high incidence of dystocia and hydrops, which decreases efficiency and increases costs. Animals delivered at term often exhibit a syndrome known as macrosomia and experience difficulties in adapting to life outside the uterus, and death is a common outcome. In the present study, 41 cloned calves that died in the neonatal period were subjected to gross and histopathological examination. Most important gross lesions were found in the liver (enlargement, congestion, yellowish color), kidneys (brownish color at surface and cut, and cysts), lungs (atelectasis, parenchymal consolidation, and secretions in bronchi and bronchioles), and heart (concentric and eccentric hypertrophy, hematic cysts, persistence of ductus arteriosus). Primary microscopic findings were seen in the liver, kidneys, and lungs from neonatal calves. In the liver, 85% of the animals exhibited hepatic degeneration. The presence of a brownish pigment within the cortical tubules of the kidneys was found in approximately 90% of the samples; the presence of this pigment has not been previously reported in cloned calves. In the lungs, a large number of animals exhibiting lesions characteristic of pneumonia (55%). These changes were the pivotal causes of death, mainly due to problems in adapting to life outside the uterus and opportunistic infections in the neonatal period. Further investigation focusing on pathological anatomical changes is necessary to map these abnormalities in cloned animals.

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Spent culture medium analysis from individually cultured bovine embryos demonstrates metabolomic differences

Perkel

Madan

2017

Zygote

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SummarySpent culture medium can provide valuable information regarding the physiological state of a bovine preimplantation embryos through non-invasive analysis of the sum/depleted metabolite constituents. Metabolomics has become of great interest as an adjunct technique to morphological and cleavage-rate assessment, but more importantly, in improving our understanding of metabolism. In this study, in vitro produced bovine embryos developing at different rates were evaluated using proton nuclear magnetic resonance (1H NMR). Spent culture medium from individually cultured embryos (2-cell to blastocyst stage) were divided into two groups based on their cleavage rate fast growing (FG) and slow growing (SG; developmentally delayed by 12–24 h), then analyzed by a 600 MHz NMR spectrometer. Sixteen metabolites were detected and investigated for sum/depletion throughout development. Data indicate distinct differences between the 4-cell SG and FG embryos for pyruvate (P < 0.05, n = 9) and at the 16-cell stage for acetate, tryptophan, leucine/isoleucine, valine and histidine. Overall sum/depletion levels of metabolites demonstrated that embryos produced glutamate, but consumed histidine, tyrosine, glycine, methionine, tryptophan, phenylalanine, lysine, arginine, acetate, threonine, alanine, pyruvate, valine, isoleucine/leucine, and lactate with an overall trend of higher consumption of these metabolites by FG groups. Principal component analysis revealed distinct clustering of the plain medium, SG, and FG group, signifying the uniqueness of the metabolomic signatures of each of these groups. This study is the first of its kind to characterize the metabolomic profiles of SG and FG bovine embryos produced in vitro using 1H NMR. Elucidating differences between embryos of varying developmental rates could contribute to a better understanding of embryonic health and physiology.

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Challenges and perspectives to enhance cattle production via in vitro techniques: focus on epigenetics and cell-secreted vesicles

Abstract: This review aim to present some clinical problems found in IVP-derived animals focusing on NT procedures and to

Cited by 3 publications

References 54 publications

Endometrial prostaglandin F2α in vitro production and its modulation regarding dominant follicle position in cattle

Endometrial prostaglandin F2α in vitro production and its modulation regarding dominant follicle position in cattle

Pathological features of cloned calves that died in the neonatal period

Spent culture medium analysis from individually cultured bovine embryos demonstrates metabolomic differences

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