Concentrações de oleandrina nas folhas de Nerium oleander de diferentes cores da floração

Pedroza, Heloísa de Paula; Ferreira, Milenna Azevedo Minhaqui; Carvalho, Júlia G.; Melo, Karla Dias Antunes; Keller, Kelly Moura; Melo, Marília Martins; Soto-Blanco, Benito

doi:10.1590/0103-8478cr20140885

Cited by 9 publications

(5 citation statements)

References 20 publications

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“…The extract also contains flavonoids, tannin and other steroids not accessed in this evaluation. Oleandrin concentrations in plant extracts were previously studied using HPLC, obtaining median values of oleandrin of 4.89mgg -1 , very similar to those found in the present study of 4.2mgg -1 , confirming the cytotoxic potential of the extract (Pedroza et al 2014). No difference has been reported in oleandrin content between plant varieties, pink, red and white flowers (Pedroza et al 2014), justifying present use of the white variety.…”

Section: Discussionsupporting

confidence: 89%

“…Oleandrin concentrations in plant extracts were previously studied using HPLC, obtaining median values of oleandrin of 4.89mgg -1 , very similar to those found in the present study of 4.2mgg -1 , confirming the cytotoxic potential of the extract (Pedroza et al 2014). No difference has been reported in oleandrin content between plant varieties, pink, red and white flowers (Pedroza et al 2014), justifying present use of the white variety. Leaves were used in the production of the extract due to its accessibility, quantity and harvesting potential (Barbosa et al 2008).…”

Section: Discussionsupporting

confidence: 89%

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Improved method for diagnosis of Nerium oleander poisoning in necropsy tissues

et al. 2018

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Nerium oleander is an ornamental cardiotoxic plant found in tropical and subtropical areas of the World. Its toxicity is related to the content of cardioactive glycosides, mainly oleandrin, found throughout the plant. The present study aimed to describe a new and improved method for oleandrin detection in tissue samples. The determination of oleandrin was made after extraction with a modified QuEChERS technique and measurement by UFLC-MS/MS. A total of 36 guinea pigs (Cavia porcellus) were distributed into 3 groups (n=12): control group that received only water orally (CON), and two treated groups that received hydroalcoholic oleander extract at doses of 150mg.kg-1 (OLE 150) and 300mg.kg-1 (OLE 300) in single oral dose. After three hours, fragments of heart, kidneys, liver and brain were collected for determination of oleandrin levels. The extraction and chromatographic procedures were effective for oleandrin detection and quantification in tissues, with retention time of 1.2 min and detection limit of 0.001μg g-1. The chromatographic analysis of treated guinea pigs indicated that oleandrin is distributed equally among the analyzed tissues. The developed methodology is a reliable, effective and rapid form of diagnosis of N. oleander poisoning based on necropsy tissue samples.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionsupporting

confidence: 89%

Improved method for diagnosis of Nerium oleander poisoning in necropsy tissues

et al. 2018

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“…The aqueous extract was again concentrated in the rotary evaportator at 68°C at 100rpm, yielding 1.545g of dry brown residue. The temperature used in the laboratory processing to obtain the extract was based on previous published studies (18,19,20) . A total of 27mg of the plant extract was diluted in 1mL Synthetic Oviduct Fluid Medium (SOFaa) for in vitro testing.…”

Section: Methodsmentioning

confidence: 99%

In vitro toxicity of Niedenzuella (Tetrapterys) multiglandulosa on bovine embryos

et al. 2022

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Niedenzuella (Tetrapterys) multiglandulosa, a vine plant found in Brazil, has been correlated to outbreaks of poisoning in cattle and buffaloes, generating economic losses related to the death due to heart failure, miscarriage, abortion, stillbirth, and neonatal mortality. The aim of this study was to examine the embryotoxic potential of the aqueous plant extract on in vitro bovine embryos. In vitro study was performed in five replicates of bovine embryo culture assigned in two groups: control, in vitro embryo culture medium without the aqueous plant extract; treated group, with addition of 2.7mg/mL of aqueous plant extract (10%) to the embryo culture on the sixth day of culture. Cleavage rate was evaluated at day 2 of the cell culture. Viability, hatchability and underdevelopment of blastocysts on the seventh, eighth, and ninth days (D7, D8, and D9, respectively) of culture were assessed under stereoscopic microscope. On day 7, blastocysts were submitted to TUNEL assay to determine apoptotic index. In vitro exposure of bovine embryos to of N. multiglandulosa resulted in reduced embryo development and survival, evaluated by dark cytoplasm indicating poor morphology and poor quality with marked reduction of hatchability. We observed a significant reduction of blastocyst production/number of cleaved embryos (60.6% vs 41.5%); reduction of blastocysts production/total number of matured bovine oocytes (35.1% vs 21.3%); and embryonic hatching rates (38.0% vs 10.0%). However, no effects were observed on the apoptotic rate. In conclusion, aqueous extract of N. multiglandulosa leaves reduces bovine embryo viability in vitro, suggesting possible detrimental effects on embryo development.

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“…The African arrow poison ouabain was found in species belonging to the genera Acokanthera and Strophanthus. The toxic oleandrin was obtained from the Apocynaceae plant species called Nerium oleander [ 28 ]. The milkweed species belong to Asclepias, and the family Asclepiadaceae, which is reported to produce the toxic cardenolide calotropin.…”

Section: Cardiac Glycosidesmentioning

confidence: 99%

Emergence of Cardiac Glycosides as Potential Drugs: Current and Future Scope for Cancer Therapeutics

et al. 2021

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Cardiac glycosides are natural sterols and constitute a group of secondary metabolites isolated from plants and animals. These cardiotonic agents are well recognized and accepted in the treatment of various cardiac diseases as they can increase the rate of cardiac contractions by acting on the cellular sodium potassium ATPase pump. However, a growing number of recent efforts were focused on exploring the antitumor and antiviral potential of these compounds. Several reports suggest their antitumor properties and hence, today cardiac glycosides (CG) represent the most diversified naturally derived compounds strongly recommended for the treatment of various cancers. Mutated or dysregulated transcription factors have also gained prominence as potential therapeutic targets that can be selectively targeted. Thus, we have explored the recent advances in CGs mediated cancer scope and have considered various signaling pathways, molecular aberration, transcription factors (TFs), and oncogenic genes to highlight potential therapeutic targets in cancer management.

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Concentrações de oleandrina nas folhas de Nerium oleander de diferentes cores da floração

Abstract: RESUMO Nerium oleander é uma planta ornamental responsável por intoxicações em animais e humanos. Todas as

Cited by 9 publications

References 20 publications

Improved method for diagnosis of Nerium oleander poisoning in necropsy tissues

Improved method for diagnosis of Nerium oleander poisoning in necropsy tissues

In vitro toxicity of Niedenzuella (Tetrapterys) multiglandulosa on bovine embryos

Emergence of Cardiac Glycosides as Potential Drugs: Current and Future Scope for Cancer Therapeutics

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