Antimicrobial Effect and Surface Tension of Some Chelating Solutions with Added Surfactants

Abstract

This study assessed the antimicrobial efficacy and surface tension of established irrigating solutions with a new experimental chelating solution in infected dentin tubes. Twenty-five specimens were randomly assigned to each of the irrigating solutions. Twenty specimens were used as negative and positive controls. After 21 days of contamination with E. faecalis, the irrigating solutions MTAD, QMiX and Tetraclean NA were delivered into each infected root canal. The solutions were removed and dentin samples were withdrawn from the root canals with sterile low-speed round burs with increasing ISO diameters. The dentin powder samples obtained with each bur were immediately collected in separate test tubes containing 3 mL of BHI broth. After that, 100 μL from each test tube was cultured on blood agar. The grown colonies were counted and recorded as colony-forming units (CFU). The surface tension of the irrigants was measured using a Cahn DCA-322 Dynamic Contact Angle Analyzer. A Kruskal Wallis nonparametric ANOVA and a Friedman test were used (p<0.05). Tetraclean NA showed lower surface tension and CFU values than MTAD and QMiX. Better antibacterial action and low surface tension were observed for Tetraclean NA, probably due to the improved penetration into the root canal and dentinal tubes

The effect of final irrigation with MTAD, QMix, and EDTA on smear layer removal and mineral content of root canal dentin

“…Namely, detergents decrease surface tension of solution and facilitate the contact of irrigant with the dentinal walls, enabling better smear layer removal. This could partially explain why EDTA, with higher surface tension compared to other investigated chelating solutions (Giardino, Ambu, Becce, Rimondini, & Morra, ; Giardino, Andrade, & Beltrami, ), was the least effective in smear layer removal from apical third. Lower smear layer capability of EDTA in the apical third can also be attributed to the fact that the chelate activity of the neutral EDTA solution is based not only on the removal of calcium ions from inorganic dentine but also on the elimination of calcium from the organic components of dentine, such as water‐soluble noncollagen proteins.…”

Evaluation of Cytotoxicity and Antibacterial Activity of a New Class of Silver Citrate-Based Compounds as Endodontic Irrigants

“…01.2023). Conversely, Tetraclean NA [64], a patented citric acid-based chelator for smear layer removal, was later reported as an irritant because of its low pH, and is thus not recommended for clinical use (Analytical Report: AAA86523, Eurofins Number: STULV18AA0037-1) by Eurofins Biolab Srl (Vimodrone, Milano, Italy).…”

Effect of QMix irrigant in removal of smear layer in root canal system: a systematic review of in vitro studies

“…After excluding studies based on title and abstract screening, 16 articles were available for full-text assessment. On careful reading, further 3 studies were excluded due to the following reasons: study done on bovine teeth [ 36 ], study that evaluated the effect of smear layer in infected dentinal tubules [ 8 ] and study that tested the bond strength of glass fiber post [ 37 ]. Finally, 13 papers were included in this systematic review.…”