Synergistic antibacterial action of β-sitosterol-d-glucopyranoside isolated from Desmostachya bipinnata leaves with antibiotics against common human pathogens

Subramaniam, Shankar; Keerthiraja, Manikandaraja; Sivasubramanian, Aravind

doi:10.1590/0102-695x20142413348

Cited by 35 publications

(16 citation statements)

References 13 publications

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“…To determine the effect of the combinatorial treatment to be synergistic, indifferent or antagonistic, FICI (a nonparametric method) was calculated (Subramaniam et al, 2014 ). The following formulas were used to calculate the FICI of a combination.…”

Section: Methodsmentioning

confidence: 99%

Combinatorial Effects of Aromatic 1,3-Disubstituted Ureas and Fluoride on In vitro Inhibition of Streptococcus mutans Biofilm Formation

et al. 2016

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Dental caries occur as a result of disequilibrium between acid producing pathogenic bacteria and alkali generating commensal bacteria within a dental biofilm (dental plaque). Streptococcus mutans has been reported as a primary cariogenic pathogen associated with dental caries. Emergence of multidrug resistant as well as fluoride resistant strains of S. mutans due to over use of various antibiotics are a rising problem and prompted the researchers worldwide to search for alternative therapies. In this perspective, the present study was aimed to screen selective inhibitors against ComA, a bacteriocin associated ABC transporter, involved in the quorum sensing of S. mutans. In light of our present in silico findings, 1,3-disubstituted urea derivatives which had better affinity to ComA were chemically synthesized in the present study for in vitro evaluation of S. mutans biofilm inhibition. The results revealed that 1,3-disubstituted urea derivatives showed good biofilm inhibition. In addition, synthesized compounds exhibited potent synergy with a very low concentration of fluoride (31.25–62.5 ppm) in inhibiting the biofilm formation of S. mutans without affecting the bacterial growth. Further, the results were supported by confocal laser scanning microscopy. On the whole, from our experimental results we conclude that the combinatorial application of fluoride and disubstituted ureas has a potential synergistic effect which has a promising approach in combating multidrug resistant and fluoride resistant S. mutans in dental caries management.

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Section: Methodsmentioning

confidence: 99%

Combinatorial Effects of Aromatic 1,3-Disubstituted Ureas and Fluoride on In vitro Inhibition of Streptococcus mutans Biofilm Formation

et al. 2016

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“…The obtained data clarifying the enhancement of H. pylori inhibition using Mix2 compared with that for the highest effective individual extract. The concept of combination between different medicinal plants or phyto-constituents and antibiotics have been reported (Aqil et al 2005;Ahmad and Aqil 2007;Subramaniam et al 2014). In addition, the aim of the current study was extend to involve dietary supplement concept, and that is why, both myrrh and licorice were chosen in mix 2 preparation in spite of their undetected antibacterial activity.…”

Section: Determination Of Phenolic Compounds and Flavonoids Of Mix2 Bmentioning

confidence: 99%

Advanced trends in controlling Helicobacter pylori infections using functional and therapeutically supplements in baby milk

et al. 2015

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Helicobacter pylori is a common human pathogen infecting about 30 % of children and 60 % of adults worldwide. It is responsible for diseases such as gastritis, peptic ulcer and gastric cancer. H. pylori treatment based on antibiotics with proton pump inhibitor, but therapy failure is shown to be higher than 20 % and is essentially due to an increasing in prevalence of antibiotic-resistant bacteria, which has led to the search for alternative therapies. In this study, we discuss the usage of natural extracts mixture as alternative or complementary agents in controlling H. pylori infection so here, we focused on the plant extracts of (Cloves, Pepper, Cumin, Sage, Pomegranate peel, Ginger, Myrrh and Licorice). To that end, Phytochemical constituents detection like Tannins, Glycosides, Alkaloids, Flavonoids, Terpenoids, Saponins, Phenolic compounds, Reducing sugars, Volatile oils, Amino acids and Proteins was demonstrated. Each plant extract was examined individually or in combination for its antimicrobial activity against H. pylori. Out of the used extracts, four mixes were prepared and tested against H. pylori. The antibacterial activities of the four mixes, represented by the diameter of inhibition clear zone, recorded 21, 39, 23 and 28 mm. The most potent mix (mix2) was chosen and mixed with baby milk as a new combination for H. pylori infections treatment in babies.

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“…Many studies have shown the isolation of antineoplastic, anti-inflammatory and antioxidant compounds in the plant [56][57][58][59][60][61][62][63] . A study conducted on the chemical composition of unripe fruits has shown the presence of compounds that are known to exhibit antioxidant, prooxidant, antiinflammatory, antibacterial, hypocholesterolemic, anti-diabetic, cytotoxic, and immunomodulatory effects 64,65,66,67,68 . This antioxidant and prooxidant nature of F. nota is supported by several studies on related species F. fiskei and F. ulmifolia and F. odorata 69 .…”

Section: Discussionmentioning

confidence: 99%

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2018

IJPSR

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Chemotherapy and radiation are the most prescribed methods of cancer treatments but these are highly toxic and also are carcinogenic thus alternative cures are being explored especially on the use of cytotoxic substances isolated from natural sources like plants known for their ethnomedicinal properties. Natural chemopreventive agents such as antioxidants isolated from plants are argued to be important in suppressing or reversing carcinogenesis and to prevent the development of invasive cancers thus research studies are now currently geared in the evaluation and determination of the cytotoxicity and antioxidant activity of plant extracts to serve as ideal cure. Since the acquisition and processing of highly cytotoxic and selective effects of plant-based compounds to cancer cells are still limited, this study was conducted on eight plant species known to have folkloric medicinal properties. The leaf ethanolic extracts were examined for their antioxidant properties and cytotoxicity against Human Non-Small Cell Lung adenocarcinoma (A549), Human Colon Carcinoma Cells (HCT116) and Chinese Hamster Normal Ovary Cells (AA8). Antioxidant properties were observed to be high in seven species. Only one species P. edule to have low antioxidant property but high toxicity to HCT116 cancer cell lines. Three other species C. ovatum, F. nota and P. odorata were both having high antioxidant properties and cytotoxicity to cancer cell lines HCT116 while four species S. contorta, T. copelandii, C. ramiflora and P. arborea have high antioxidant but relative very low toxicity to HCT116 cancer cell lines. While two species namely, C. ovatum and P. edule were cytotoxic to HCT116 cancer cell lines, only C. ovatum was found to be cytotoxic for both A549 cancer cell lines thus could be a good candidate as source of chemotherapeutic and chemopreventive compounds. The results that showed seven out of the eight species have high percentage of antioxidants can be argued that these species are candidate species as sources of chemopreventive compounds. Further studies are still needed to determine the compounds from these species that confer selective cytotoxicity to cancer cell lines.

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Synergistic antibacterial action of β-sitosterol-d-glucopyranoside isolated from Desmostachya bipinnata leaves with antibiotics against common human pathogens

Cited by 35 publications

References 13 publications

Combinatorial Effects of Aromatic 1,3-Disubstituted Ureas and Fluoride on In vitro Inhibition of Streptococcus mutans Biofilm Formation

Combinatorial Effects of Aromatic 1,3-Disubstituted Ureas and Fluoride on In vitro Inhibition of Streptococcus mutans Biofilm Formation

Advanced trends in controlling Helicobacter pylori infections using functional and therapeutically supplements in baby milk

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