Does the Association of Tacrolimus and Mycophenolate Mofetil Change the Healing of the Abdominal Wall? Study in Rats Submitted to Ischemia and Kidney Reperfusion

Abstract

Background: Tacrolimus and mycophenolate mofetil are immunosuppressive agents widely used on the postoperative period of the transplants. Aim: To evaluate the influence of the association of them on the abdominal wall healing in rats. Methods: Thirty-six Wistar rats were randomly assigned in three groups of 12. On the early postoperative period, four of the control group and three of the experimental groups died. The three groups were nominated as follow: control group (GC, n=8); group I (GI, n=11, standard operation, mycophenolate mofetil and tacrolimus); group II (GII, n=10, standard operation, mycophenolate mofetil and tacrolimus). The standard operation consisted of right total nephrectomy and 20 min ischemia of the left kidney followed by reperfusion. Both NaCl 0.9% and the immunosuppressive agents were administered starting on the first postoperative day and continuing daily until the day of death on the 14th day. On the day of their deaths, two strips of the anterior abdominal wall were collected and submitted to breaking strength measurement and histological examination. Results: There were no significant differences in wound infection rates (p=0,175), in the breaking strength measurement and in the histological examination among the three groups. Conclusion: The combination of the immunosuppressive agents used in the study associated with renal ischemia and reperfusion does not interfere in the abdominal wall healing of rats.

Drogas Imunossupressoras Afetam Os Núcleos Hipotalâmicos Envolvidos Na Ingestão Alimentar ? Estudo Experimental

“…Além disso, existem poucos relatos sobre o caráter neurotóxico dos imunossupressores no hipotálamo. Assim, a abordagem quanto ao TAC e MMF como fatores etiológicos relacionados à obesidade é mínima 27 . As correlações diretas entre imunossupressores, núcleos hipotalâmicos e obesidade ainda não são claras, conforme descrito na literatura 1 , as quais referem que o uso de imunossupressores como fator de risco para excesso de peso pós-transplante ainda é controverso.…”

“…Furthermore, there are only few reports about the immunosuppressant neurotoxic character in the hypothalamus. Thus, the approach regarding TAC and MMF as etiological factors related to obesity is minimal 27 . The direct correlations between immunosuppressants, hypothalamic nuclei, and obesity are still unclear, as described in the literature 1 , referring to the use of immunosuppressive drugs as a risk factor for excess weight post-transplant which is still controversial.…”