Immunomodulating Effects of the Purified Hev B 13 Fraction on Septic Rats

Abstract

Background: Sepsis is a potentially life-threatening complication of an infection that occurs when chemicals released into the bloodstream to fight the infection trigger inflammatory responses throughout the body, especially in the acute phase of the disease, producing excessive pro-inflammatory cytokines, leading to multiple organ injury and death. The Hev b 13 fraction has demonstrated biological activity capable of inducing IL-10 production and shrinking inflammatory disease lesions. Aim: To investigate the immunomodulating effects of the Hev b 13 fraction on septic rats. Methods: Acinetobacter baumannii was injected into the peritoneal cavity of the animals after sustaining a lesion in the pancreas, with the stomach as an entry point. After 10 h of infection, they were euthanized for blood and lung collection, followed by total and differential leukocyte count, determination of cytokine level and histopathological analysis. Results: Administering a single dose of the Hev b 13 fraction 2 h after sepsis induction significantly decreased total leukocyte count. Higher IL-10 and IL-4 and lower IL-6 production shrank the lung tissue lesions compared to the control groups. Conclusion: The Hev b 13 fraction exhibits an anti-inflammatory tendency, with potential for sepsis treatment.

Effects of Intraperitoneal Glutamine in the Treatment of Experimental Sepsis

“…With the progression of sepsis, three types of changes occur: infection, inflammatory response and hemodynamic change. However, even today the treatment performed is not directly related to the inflammatory response, since it is done only with antibiotics and vasoactive drugs 1 , 3 . In this sense, the action of the amino acid glutamine (Gln) can be very relevant in sepsis, since it would act in the current inflammatory reaction 12 .…”