2015
DOI: 10.1590/01000683rbcs20140689
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Abstract: The use of leaf total nitrogen concentration as an indicator for nutritional diagnosis has some limitations. The objective of this study was to determine the reliability of total N concentration as an indicator of N status for eucalyptus clones, and to compare it with alternative indicators. a greenhouse experiment was carried out in a randomized complete block design in a 2 × 6 factorial arrangement with plantlets of two eucalyptus clones (140 days old) and six levels of N in the nutrient solution. In additio… Show more

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Cited by 10 publications
(6 citation statements)
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References 29 publications
(47 reference statements)
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“…Forde and Lea (2007) suggested that at different stages of growth there may be great variation in the level of soluble amino acids, but glutamate varies less than other amino acids, particularly glutamine. For clone VM-01, the greater glutamine (Table 1) and glutamate ( Fig 3b) concentrations in plants grown at a higher N rate are in accordance with greater NH 4 + concentrations (Ferreira et al, 2015a). High NH 4 + concentration can be toxic to plants (Marschner, 2012) and thus it is incorporated into glutamine and glutamate (Ireland and Lea, 1999).…”
Section: Root Amino Acidsmentioning
confidence: 64%
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“…Forde and Lea (2007) suggested that at different stages of growth there may be great variation in the level of soluble amino acids, but glutamate varies less than other amino acids, particularly glutamine. For clone VM-01, the greater glutamine (Table 1) and glutamate ( Fig 3b) concentrations in plants grown at a higher N rate are in accordance with greater NH 4 + concentrations (Ferreira et al, 2015a). High NH 4 + concentration can be toxic to plants (Marschner, 2012) and thus it is incorporated into glutamine and glutamate (Ireland and Lea, 1999).…”
Section: Root Amino Acidsmentioning
confidence: 64%
“…This fact was associated with a higher total N concentration (Fig 1b) and greater protein synthesis (Ferreira et al, 2015b) and photosynthetic rate (Ferreira et al, 2015c), which resulted in an increase in the total dry matter production of the clones (Fig 1c) since the amino acids taken up are sources of C skeletons for plant metabolism (Majerowicz and Kerbauy, 2002). The smaller concentrations in leaf amino acids of the plants grown at lower N rates (0 and 0.74 mmol L -1 of NH 4 NO 3 ) were also observed through visual symptoms of generalized chlorosis, since total N concentration (Fig 1b) and chlorophyll levels were both lower (Ferreira et al, 2015a). Chlorosis is developed following the removal of the sink, causing depletion in total N, proline, arginine, and serine (Schaffer et al, 1986).…”
Section: Shoot Amino Acidsmentioning
confidence: 93%
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“…Immediately following photosynthesis measurements on 3 June 2014 and 10 June, 9 July, 21 July and 22 August 2015, three leaves were excised from each sapling, dried at 60°C for 72 hr, weighed and homogenized for nitrogen concentration analysis via flash combustion (Thermoquest NC 2500 autoanalyser). We use foliar nitrogen as an indicator of plant nitrogen status and proxy for plant nitrogen uptake (de Ferreira, Novais, Médice, de Barros, & Silva, ). Litterfall production and stem growth were assessed as measures of above‐ground productivity in 2013 (to determine baseline values before treatment was initiated) and in 2014 only, as herbivory damage in 2015 precluded comparable measurements due to stem regrowth and early season leaf flush failure.…”
Section: Methodsmentioning
confidence: 99%
“…Tree leaves were collected biweekly beginning in May 2012 and continuing to the end of September 2014 to determine effects of the irrigation system and N treatment on leaf N concentration as an indicator of tree N status [32]. Data for 2015 were reported in Ayars et al [17].…”
Section: Plant Measurementsmentioning
confidence: 99%