Biocontrol of Sclerotinia sclerotiorum and white mold of soybean using saprobic fungi from semi-arid areas of Northeastern Brazil

Barros, Daiane Cristina Martins; Fonseca, Inês Cristina de Batista; Balbi-Peña, María Isabel; Pascholati, Sérgio Florentino; Peitl, Douglas Casaroto

doi:10.1590/0100-5405/2086

Cited by 13 publications

(24 citation statements)

References 13 publications

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“…Those authors found that T. stromaticum had the highest antagonistic effect. Balbi-Peña et al (2012) and Barros et al (2015) studied in vitro antagonistic effect of saprobe fungi S. globosa and M. levispora on Sclerotinia sclerotiorum. Also, C. virescens var.…”

Section: P Hispidula Z Echinosporum C Virescensmentioning

confidence: 99%

“…Saprobe fungi have desirable characteristics for use in biological control (BARROS et al, 2015) and a resistance-inducing potential YEDIDIA et al, 2003). They can secrete pectinases, induce oligogalacturonides release; and activate the plant defense responses (BARROS et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

“…They can secrete pectinases, induce oligogalacturonides release; and activate the plant defense responses (BARROS et al, 2015). The use of saprobe fungi from semi-arid Brazilian caatinga ecoregion was favored due to their potential as biocontrol agents, including their ability to remove nutrients from dead tissue, resistance to water restriction, and competitiveness with other microorganisms (PINTO, 2013;RESENDE et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Biological control of bacterial spot of tomato by saprobe fungi from semi-arid areas of northeastern Brazil

Peitl

Araújo

Gonçalves

et al. 2017

SCA

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Tomato bacterial spot caused by Xanthomonas spp., is a common disease in tomato fields that causes significant economic losses. Due to the difficulty with control of bacterial spot by conventional methods, new techniques such as biological control and induction of resistance are gaining prominence. This study aimed to select saprobe fungi from semi-arid regions of the Brazilian Northeast for the biological control of bacterial spot of tomato. To select the best isolates to control bacterial spot, a greenhouse experiment was initially conducted. Tomato plants ('Santa Cruz Kada') were treated with filtrates of 25 saprobe fungi and inoculated three days later with Xanthomonas euvesicatoria. Filtrates of Memnoniella levispora, Periconia hispidula, Zygosporium echinosporum, and Chloridium virescens var. virescens were selected as the most effective. Filtrates and volatile compounds from these four isolates were tested for their antibacterial activity in cultures of X. euvesicatoria and in tomato plants ('Santa Cruz Kada') inoculated with X. euvesicatoria. In vitro, the addition of nonvolatile fungal metabolites into the culture medium at 5% and 50% (v/v) inhibited bacterial growth by 28.9% and 53.8%, respectively. The volatile compounds produced by C. virescens var. virescens reduced the number of colony-forming units of X. euvesicatoria by 25.9%. In vivo, all treatments reduced from 62.4 to 71.3% the area under bacterial spot progress curve, showing the same control efficacy as the commercial resistance inducer used as a positive control (acibenzolar-S-methyl). Systemicity of the fungal filtrates was confirmed in a separate experiment, where application of the treatments exclusively to the third leaf decreased the severity of the disease on the fourth leaf (except for C. virescens var. virescens). These results show that M. levispora, P. hispidula, Z. echinosporum, and C. virescens var. virescens are potential biocontrol agents against tomato bacterial spot. Further studies are necessary to elucidate the disease control mechanisms of these saprobe fungi. Key words: Acibenzolar-S-methyl. Antibiosis. Fungal metabolite. Xanthomonas euvesicatoria. ResumoA mancha bacteriana do tomateiro causada por Xanthomonas spp. é uma doença frequente nos campos de cultivo, provocando importantes perdas econômicas. Devido à dificuldade de controle com os métodos tradicionais, novas ferramentas de manejo para esta doença, como o controle biológico e a indução de resistência vêm ganhando destaque. O objetivo desse trabalho foi a seleção de fungos sapróbios do semiárido nordestino brasileiro para o controle biológico da mancha bacteriana do tomateiro. Inicialmente, com o objetivo de selecionar os isolados que apresentassem melhor controle da mancha bacteriana, foi montado um ensaio em casa de vegetação com tomateiros ('Santa Cruz Kada') tratados com os filtrados de 25 fungos sapróbios e inoculados com a bactéria Xanthomonas euvesicatoria três dias depois. Foram selecionados os isolados de Memnoniella levispora, Periconia hispidul...

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Section: P Hispidula Z Echinosporum C Virescensmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Biological control of bacterial spot of tomato by saprobe fungi from semi-arid areas of northeastern Brazil

Peitl

Araújo

Gonçalves

et al. 2017

SCA

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“…A. C. Almeida, Izabel, & Gusmão, 2011;Leão-Ferreira, Pascholati, Gusmão, & Castañeda Ruiz, 2013;Santa Izabel & Gusmão, 2018). Some of these saprobes were being used as potential biological control agents and resistance inducers (Resende, Milagres, Rezende, Aucique-Perez, & Rodrigues, 2015;Barros, Fonseca, Balbi-Peña, Pascholati, & Peitl, 2015;Rodríguez et al, 2016;Peitl et al, 2017;Ribeiro et al, 2018;Botrel et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Antagonism of saprobe fungi from semiarid areas of the Northeast of Brazil against Sclerotinia sclerotiorum and biocontrol of soybean white mold

Peitl

Sumida

Gonçalves

et al. 2020

Semina: Ciênc. Agrár.

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The antagonistic activity of 25 saprobe fungi from semiarid areas of Northeast Brazil was evaluated against Sclerotinia sclerotiorum (Lib.) de Bary (Helotiales: Sclerotiniaceae). Four fungi [Myrothecium sp. Tode (Hypocreales: Stachybotryaceae) isolate 2, Volutella minima Höhn. (Hypocreales: Nectriaceae), Phialomyces macrosporus P.C. Misra & P.H.B. Talbot (Pezizomycotina) and Dictyosporium tetraseriale Goh, Yanna & K.D. Hyde (Pleosporales: Dictyosporiaceae)] were selected and further tested their ability to inhibit mycelial growth, sclerotia formation and ascospore germination of S. sclerotiorum and to control white mold on soybean plants. V. minima and P. macrosporus filtrates at 50% effectively suppressed mycelial growth and Myrothecium sp. isolate 2 completely suppressed sclerotia formation and inhibited ascospore germination by over 95%, the same result as commercial fungicide fluazinam. Soybean plants pre-treated with Myrothecium sp. isolate 2, P. macrosporus, and V. minima and inoculated with S. sclerotiorum showed a reduction of 55.8%, 79.7%, and 83.2% of area under disease progress curve (AUDPC) of white mold, respectively, in relation to water. Collectively, these results underline the antagonistic activity of V. minima, P. macrosporus, and Myrothecium sp. isolate 2 against S. sclerotiorum and their potential as biocontrol agents of soybean white mold.

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“…A collection of saprophytic fungi isolated from leaf litter of an arid region of Brazil has proven to be efficient to control plant disease. Some of these isolates is a potential agent of biocontrol in coffee (RODRÍGUEZ et al, 2016), white mold in soybean (Barros et al, 2015) and brown eye spot (Laborde et al, 2018). However, the saprobe fungi potential for bacterial blight control has not been attempted for many species.…”

Section: Introductionmentioning

confidence: 99%

SAPROBIC FUNGI AS BIOCONTROL AGENTS OF HALO BLIGHT (Pseudomonas syringae pv. garcae) IN COFFEE CLONES

Botrel

Laborde²,

Medeiros

et al. 2018

C.Sci.

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Halo blight caused by Pseudomonas syringae pv. garcae is a limiting disease in coffee production. There are few efficient commercial products on the market to control this disease, and therefore, the prospection of different biocontrol agents is a promising alternative. The objectives in this study were (i) to select saprobic fungi with the potential to control halo blight in coffee clones, and (ii) to evaluate the contributions of induced resistance as control mechanisms. Plants were sprayed with Gonytrichum chlamydosporium, Phialomyces macrosporus, and Moorella speciosa 7 d before inoculation with Pseudomonas. syringae pv. garcae. The area under the halo blight progress curve (AUDPC) and plant growth parameters were evaluated. M. speciose and G. clamydosporium did not reduce the AUDPC and even reduced plant growth in none of the trails compared to the water control. P. macrosporus consistently reduced AUDPC by 42-72% and increased plant height by 40%. Thereafter, the contributions of induced resistance was evaluated for the P. macrosporus, selected as the most promising biocontrol agent.. In order to determine induced resistance, phenylalanine ammonia lyase (PAL), peroxidase (POX), and ascorbate peroxidase (APX) activity of plant leaves were measured at two time points after stress challenge. Enzyme activity evaluation demonstrated high activity of POX and PAL at seven days after treatment with the saprobe, and high APX activity after 14 days. The results of this study indicate that P. macrosporus has the potential to be used in the management of coffee halo blight in seedling production, and one mechanism likely involved is induced resistance.

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Biocontrol of Sclerotinia sclerotiorum and white mold of soybean using saprobic fungi from semi-arid areas of Northeastern Brazil

Cited by 13 publications

References 13 publications

Biological control of bacterial spot of tomato by saprobe fungi from semi-arid areas of northeastern Brazil

Biological control of bacterial spot of tomato by saprobe fungi from semi-arid areas of northeastern Brazil

Antagonism of saprobe fungi from semiarid areas of the Northeast of Brazil against Sclerotinia sclerotiorum and biocontrol of soybean white mold

SAPROBIC FUNGI AS BIOCONTROL AGENTS OF HALO BLIGHT (Pseudomonas syringae pv. garcae) IN COFFEE CLONES

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